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A kind of sludge aerobic composting compound bacterial agent and its preparation method and application

A technology of aerobic composting and compound bacterial agents, applied in the direction of sewage/sludge fertilizer, biochemical equipment and methods, applications, etc., can solve the problems of slow degradation rate of organic matter, short maintenance time of high temperature period, long time to reach high temperature period, etc. problems, to achieve the effects of reducing the content of toxic substances, high seed germination index, and increasing the rate

Active Publication Date: 2022-06-24
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, anaerobic digestion is the most widely used, but there are potential safety hazards and high requirements for operation and management; composting is a biological treatment method, because it can make full use of the components in sludge, turn waste into treasure, and is conducive to the establishment of a recycling system. Economical, but most of the bacterial agents currently used for sludge composting have the defects of slow decomposing speed, long time to reach high temperature period, short high temperature period maintenance time, slow organic matter degradation rate and poor decomposing effect, and it is difficult to achieve a better decomposing degree

Method used

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  • A kind of sludge aerobic composting compound bacterial agent and its preparation method and application
  • A kind of sludge aerobic composting compound bacterial agent and its preparation method and application
  • A kind of sludge aerobic composting compound bacterial agent and its preparation method and application

Examples

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Effect test

Embodiment 1

[0034] A sludge aerobic composting compound bacterial agent, comprising Paenibacillus barrenii ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitritidis ( Geobacillus thermodenitrificans ) HX-4;

[0035] The Paenibacillus barrenii ( Paenibacillus barengoltzii ) The preservation number of TZ-1 is CGMCC No. 23696, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee on November 1, 2021, and the deposit address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing; the said Bacillus licheniformis ( Bacillus licheniformis ) The preservation number of TD-4 is CGMCC NO.24154, and it was deposited in the General Microbiology Center of the China Microorganism Culture Collection Management Committee on December 21, 2021. The deposit address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; Geobacillus th...

Embodiment 2

[0047] A composite bacterial agent for aerobic composting of sludge, comprising Paenibacillus barrenii in Example 1 ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitritidis ( Geobacillus thermodenitrificans ) HX-4;

[0048] The preparation method of the above-mentioned sludge aerobic composting compound bacterial agent is:

[0049] 1) Preparation of medium: LB liquid medium: sodium chloride 8g, peptone 8g, yeast extract 4g, distilled water to make up to 1000mL;

[0050] Lipase fermentation medium: glucose 4g, peptone 1.8g, urea 5g, dipotassium hydrogen phosphate trihydrate 0.8g, ammonium sulfate 0.8g, magnesium sulfate heptahydrate 0.4g, tributyrin 8mL, distilled water to make up to 1000mL;

[0051] Protease fermentation medium: glucose 1.8g, peptone 1.8g, calcium carbonate 1.8g, ferrous sulfate 0.4g, ammonium sulfate 0.8g, magnesium sulfate heptahydrate 1.8g, dipotassium hydrogen phosphate 1.8g, potass...

Embodiment 3

[0059] A composite bacterial agent for aerobic composting of sludge, comprising Paenibacillus barrenii in Example 1 ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitritidis ( Geobacillus thermodenitrificans ) HX-4;

[0060] The preparation method of the above-mentioned sludge aerobic composting compound bacterial agent is:

[0061] 1) Preparation of medium: LB liquid medium: sodium chloride 12g, peptone 12g, yeast extract 6g, distilled water to make up to 1000mL;

[0062] Lipase fermentation medium: glucose 6g, peptone 2.2g, urea 7g, dipotassium hydrogen phosphate trihydrate 1.2g, ammonium sulfate 1.2g, magnesium sulfate heptahydrate 0.6g, tributyrin 12mL, distilled water to make up to 1000mL;

[0063] Protease fermentation medium: glucose 2.2g, peptone 2.2g, calcium carbonate 2.2g, ferrous sulfate 0.6g, ammonium sulfate 1.2g, magnesium sulfate heptahydrate 2.2g, dipotassium hydrogen phosphate 2.2g, pot...

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Abstract

The invention relates to the technical field of microbial fermentation, and specifically discloses a sludge aerobic composting composite bacterial agent, a preparation method and application thereof. The composite bacterial agent includes Paenibacillus baronii TZ-1, Bacillus licheniformis TD-4 and Geobacillus thermodenitrifying HX-4. The sludge aerobic composting composite bacterial agent provided by the present invention is used for sludge composting and has the advantages of fast heating speed, long maintenance time in high temperature period, high fermentation temperature, fast decomposing speed, high organic matter degradation rate, fast moisture content drop and high seed germination index Advantages can significantly shorten the composting cycle and improve the quality of composting.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a composite bacterial agent for aerobic composting of sludge and a preparation method and application thereof. Background technique [0002] With the continuous improvement of the level of urbanization, the amount of urban sewage treatment is also increasing, and a large amount of sludge is produced along with it. Sludge has the characteristics of large capacity, instability, easy corruption and odor. If it is not treated effectively, it will cause serious secondary environmental pollution to its stacking and discharge areas, and even cause irreversible poisoning to the soil. The growth of surrounding crops and the pollution of crops. [0003] Traditional sludge treatment methods include incineration, landfill and resource utilization. The current treatment methods mostly use incineration method and landfill method, but incineration method has a huge investment a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05F7/00C12R1/10C12R1/01
CPCY02A40/20
Inventor 韩永辉滕志楠崔建升李清扬黄亚丽秦学安鸿雪李再兴周淋友王艳魁刘馨岳杨添琪吕建伟刘峰
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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