A kind of sludge aerobic composting compound bacterial agent and its preparation method and application
A technology of aerobic composting and compound bacterial agents, applied in the direction of sewage/sludge fertilizer, biochemical equipment and methods, applications, etc., can solve the problems of slow degradation rate of organic matter, short maintenance time of high temperature period, long time to reach high temperature period, etc. problems, to achieve the effects of reducing the content of toxic substances, high seed germination index, and increasing the rate
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Embodiment 1
[0034] A sludge aerobic composting compound bacterial agent, comprising Paenibacillus barrenii ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitritidis ( Geobacillus thermodenitrificans ) HX-4;
[0035] The Paenibacillus barrenii ( Paenibacillus barengoltzii ) The preservation number of TZ-1 is CGMCC No. 23696, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee on November 1, 2021, and the deposit address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing; the said Bacillus licheniformis ( Bacillus licheniformis ) The preservation number of TD-4 is CGMCC NO.24154, and it was deposited in the General Microbiology Center of the China Microorganism Culture Collection Management Committee on December 21, 2021. The deposit address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; Geobacillus th...
Embodiment 2
[0047] A composite bacterial agent for aerobic composting of sludge, comprising Paenibacillus barrenii in Example 1 ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitritidis ( Geobacillus thermodenitrificans ) HX-4;
[0048] The preparation method of the above-mentioned sludge aerobic composting compound bacterial agent is:
[0049] 1) Preparation of medium: LB liquid medium: sodium chloride 8g, peptone 8g, yeast extract 4g, distilled water to make up to 1000mL;
[0050] Lipase fermentation medium: glucose 4g, peptone 1.8g, urea 5g, dipotassium hydrogen phosphate trihydrate 0.8g, ammonium sulfate 0.8g, magnesium sulfate heptahydrate 0.4g, tributyrin 8mL, distilled water to make up to 1000mL;
[0051] Protease fermentation medium: glucose 1.8g, peptone 1.8g, calcium carbonate 1.8g, ferrous sulfate 0.4g, ammonium sulfate 0.8g, magnesium sulfate heptahydrate 1.8g, dipotassium hydrogen phosphate 1.8g, potass...
Embodiment 3
[0059] A composite bacterial agent for aerobic composting of sludge, comprising Paenibacillus barrenii in Example 1 ( Paenibacillus barengoltzii ) TZ-1, Bacillus licheniformis ( Bacillus licheniformis ) TD-4 and Geobacillus thermodenitritidis ( Geobacillus thermodenitrificans ) HX-4;
[0060] The preparation method of the above-mentioned sludge aerobic composting compound bacterial agent is:
[0061] 1) Preparation of medium: LB liquid medium: sodium chloride 12g, peptone 12g, yeast extract 6g, distilled water to make up to 1000mL;
[0062] Lipase fermentation medium: glucose 6g, peptone 2.2g, urea 7g, dipotassium hydrogen phosphate trihydrate 1.2g, ammonium sulfate 1.2g, magnesium sulfate heptahydrate 0.6g, tributyrin 12mL, distilled water to make up to 1000mL;
[0063] Protease fermentation medium: glucose 2.2g, peptone 2.2g, calcium carbonate 2.2g, ferrous sulfate 0.6g, ammonium sulfate 1.2g, magnesium sulfate heptahydrate 2.2g, dipotassium hydrogen phosphate 2.2g, pot...
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