Method for determining scheme for biomineralization treatment of copper-containing wastewater and wastewater treatment method
A technology of biomineralization and determination method, applied in the field of environmental engineering, can solve the problems of inability to decompose urea, release into the surrounding soil, and high toxicity, and achieve the effect of avoiding poisoning, promoting rapid formation, and being easy to popularize and apply.
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Embodiment 1
[0034] This embodiment provides a method for determining the scheme of biomineralization treatment of copper-containing wastewater, including:
[0035] Step 1. Bacteria activation: put 100mL activation medium in a 250mL Erlenmeyer flask, place the lyophilized powder of bacteria in the activation medium, seal it with a biological sealing film and put it in a constant temperature shaking incubator, at 30°C And the shaker speed is 190rpm and cultivated for 28h; the pH of the activation medium is 9; the activation medium includes: yeast extract 20g / L, urea 20g / L, ammonium chloride 10g / L, manganese sulfate monohydrate 10mg / L and nickel chloride hexahydrate 24mg / L; The yeast extract, ammonium chloride, manganese sulfate monohydrate and nickel chloride hexahydrate are all sterilized, and the urea is filtered through a 0.22 micron filter head; The bacterium is Bacillus sarcina pasteurii; the bacillus sarcina pasteurii is purchased from the China General Microorganism Culture Collectio...
Embodiment 2
[0048] This embodiment provides a method for determining the scheme of biomineralization treatment of copper-containing wastewater, including:
[0049] Step 1. Bacteria activation: put 100mL activation medium in a 250mL Erlenmeyer flask, place the lyophilized powder of bacteria in the activation medium, seal it with a biological sealing film and put it in a constant temperature shaking incubator, at 25°C And the shaker speed is 200rpm under the condition of cultivating 24h; the pH of the activation medium is 8; the activation medium includes: yeast extract 18g / L, urea 18g / L, ammonium chloride 8g / L, manganese sulfate monohydrate 8mg / L and nickel chloride hexahydrate 20mg / L; The yeast extract, ammonium chloride, manganese sulfate monohydrate and nickel chloride hexahydrate are all sterilized, and the urea is filtered through a 0.22 micron filter head; The bacterium is Bacillus sarcina pasteurii; the bacillus sarcina pasteurii is purchased from the China General Microorganism Cul...
Embodiment 3
[0055] This embodiment provides a method for determining the scheme of biomineralization treatment of copper-containing wastewater, including:
[0056] Step 1. Bacteria activation: put 100mL activation medium in a 250mL Erlenmeyer flask, place the lyophilized powder of bacteria in the activation medium, seal it with a biological sealing film and put it in a constant temperature shaking incubator, at 35°C And the shaker speed is 180rpm and cultured for 30h; the pH of the activation medium is 10; the activation medium includes: yeast extract 22g / L, urea 22g / L, ammonium chloride 12g / L, manganese sulfate monohydrate 12mg / L and nickel chloride hexahydrate 28mg / L; The yeast extract, ammonium chloride, manganese sulfate monohydrate and nickel chloride hexahydrate are all sterilized, and the urea is filtered through a 0.22 micron filter head; The bacterium is Bacillus sarcina pasteurii; the bacillus sarcina pasteurii is purchased from the China General Microorganism Culture Collection...
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Abstract
Description
Claims
Application Information
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