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Method for determining scheme for biomineralization treatment of copper-containing wastewater and wastewater treatment method

A technology of biomineralization and determination method, applied in the field of environmental engineering, can solve the problems of inability to decompose urea, release into the surrounding soil, and high toxicity, and achieve the effect of avoiding poisoning, promoting rapid formation, and being easy to popularize and apply.

Active Publication Date: 2022-04-22
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment options for heavy metal polluted water bodies are mainly physical and chemical processes, such as chemical precipitation, adsorption, membrane filtration, coagulation and flocculation, and electrochemical treatment. These processes generally have problems such as high energy consumption and possible secondary pollution.
Although bioremediation based on plant extraction, fixation, volatilization and filtration is mild to soil modification, it is easy to cause the accumulation of heavy metals in plants, and may be released into the surrounding soil under certain conditions.
[0004] However, traditional restoration methods have encountered relatively large problems in the restoration of copper ions, which are mainly manifested in the following two aspects: On the one hand, the high urea requirement for bacterial copper resistance will make the solution more alkaline, and ammonium ions A large amount of cupro-ammonia complexes are generated to affect its repair efficiency; on the other hand, copper ions have a great toxicity to bacteria and their product urease, and it is difficult to keep them active in high-concentration copper ions. Bacteria and If the product urease loses its activity, it will not be able to decompose urea, so as to achieve the purpose of copper ion repair

Method used

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  • Method for determining scheme for biomineralization treatment of copper-containing wastewater and wastewater treatment method
  • Method for determining scheme for biomineralization treatment of copper-containing wastewater and wastewater treatment method
  • Method for determining scheme for biomineralization treatment of copper-containing wastewater and wastewater treatment method

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Embodiment 1

[0034] This embodiment provides a method for determining the scheme of biomineralization treatment of copper-containing wastewater, including:

[0035] Step 1. Bacteria activation: put 100mL activation medium in a 250mL Erlenmeyer flask, place the lyophilized powder of bacteria in the activation medium, seal it with a biological sealing film and put it in a constant temperature shaking incubator, at 30°C And the shaker speed is 190rpm and cultivated for 28h; the pH of the activation medium is 9; the activation medium includes: yeast extract 20g / L, urea 20g / L, ammonium chloride 10g / L, manganese sulfate monohydrate 10mg / L and nickel chloride hexahydrate 24mg / L; The yeast extract, ammonium chloride, manganese sulfate monohydrate and nickel chloride hexahydrate are all sterilized, and the urea is filtered through a 0.22 micron filter head; The bacterium is Bacillus sarcina pasteurii; the bacillus sarcina pasteurii is purchased from the China General Microorganism Culture Collectio...

Embodiment 2

[0048] This embodiment provides a method for determining the scheme of biomineralization treatment of copper-containing wastewater, including:

[0049] Step 1. Bacteria activation: put 100mL activation medium in a 250mL Erlenmeyer flask, place the lyophilized powder of bacteria in the activation medium, seal it with a biological sealing film and put it in a constant temperature shaking incubator, at 25°C And the shaker speed is 200rpm under the condition of cultivating 24h; the pH of the activation medium is 8; the activation medium includes: yeast extract 18g / L, urea 18g / L, ammonium chloride 8g / L, manganese sulfate monohydrate 8mg / L and nickel chloride hexahydrate 20mg / L; The yeast extract, ammonium chloride, manganese sulfate monohydrate and nickel chloride hexahydrate are all sterilized, and the urea is filtered through a 0.22 micron filter head; The bacterium is Bacillus sarcina pasteurii; the bacillus sarcina pasteurii is purchased from the China General Microorganism Cul...

Embodiment 3

[0055] This embodiment provides a method for determining the scheme of biomineralization treatment of copper-containing wastewater, including:

[0056] Step 1. Bacteria activation: put 100mL activation medium in a 250mL Erlenmeyer flask, place the lyophilized powder of bacteria in the activation medium, seal it with a biological sealing film and put it in a constant temperature shaking incubator, at 35°C And the shaker speed is 180rpm and cultured for 30h; the pH of the activation medium is 10; the activation medium includes: yeast extract 22g / L, urea 22g / L, ammonium chloride 12g / L, manganese sulfate monohydrate 12mg / L and nickel chloride hexahydrate 28mg / L; The yeast extract, ammonium chloride, manganese sulfate monohydrate and nickel chloride hexahydrate are all sterilized, and the urea is filtered through a 0.22 micron filter head; The bacterium is Bacillus sarcina pasteurii; the bacillus sarcina pasteurii is purchased from the China General Microorganism Culture Collection...

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Abstract

The invention discloses a determination method of a biomineralization copper-containing wastewater treatment scheme and a wastewater treatment method. The determination method comprises the following steps: step 1, activating bacteria; step 2, cryopreservation; step 3, carrying out subculture; step 4, determining treatment conditions, specifically, according to a preset group, adding the subcultured bacterial liquid into the copper-containing wastewater, treating for 48 hours, measuring the urease activity, pH and copper ion concentration of the system according to a preset time point in the treatment process, and determining a treatment scheme; the urease is produced by the sporosarcina pasteurii; the setting method of the preset group comprises the following steps: the copper-containing wastewater is divided into multiple groups according to the copper ion concentration, each group of copper-containing wastewater comprises multiple samples, and the bacterial liquid proportion comprises multiple levels by taking the bacterial liquid proportion as a factor. The method can effectively and accurately obtain a treatment scheme for efficiently treating the copper-polluted wastewater, and can be effectively applied to guidance of a heavy metal-containing wastewater treatment process.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering, and in particular relates to a method for determining a scheme for biomineralization treatment of copper-containing wastewater and the wastewater treatment method. Background technique [0002] The process of urbanization, industrialization, mining and agricultural intensification has intensified the release of heavy metals to the environment, and more and more heavy metals directly or indirectly endanger human health through soil, water, atmosphere and other media. In recent years, the problem of heavy metal pollution has become increasingly serious, which has aroused widespread concern from the whole society. Heavy metal pollution mainly comes from mineral mining and smelting, metal processing, machinery manufacturing, and steel production. For example, the copper content in the wastewater discharged from the electroplating industry and metal processing is relatively high, ra...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C02F101/20C12R1/01
CPCC02F3/34C02F3/342C12N1/20C02F2101/20C02F2305/06Y02W10/10
Inventor 谢毅鑫郑文杰薛中飞
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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