Method for improving fermentation yield and conversion rate of threonine
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A technology for increasing threonine and conversion rate, which is applied in the field of threonine fermentation, can solve problems such as impact, and achieve the effects of high efficiency, simple process operation, high stability and high degree of automation
Pending Publication Date: 2022-04-22
江苏元易邦生物科技有限公司
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[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a method for increasing the fermentation yield and conversion rate of threonine, which effectively solves the problem that the direct fermentation method of Escherichia coli is the industrial production of L-threonine at present. The preferred technology, during the fermentation process, excessive accumulation of metabolic by-products such as acetic acid and NH4+ will have a serious impact on bacterial growth, bacterial viability and acid production
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Embodiment 1
[0034] A method for increasing threonine fermentation yield and conversion rate, comprising the following steps:
[0036] S2, slant culture: take 0.2% glucose, 1.0% peptone, 0.5% yeast powder, 1.0% beef extract, 0.5% NaCl, 1.8% agar, put it into the slant medium, adjust the pH value to 7.2, and Put the slant medium into the heating box, adjust the temperature to 36°C, and cultivate for 20 hours. After passing the inspection, put it in the refrigerator for future use;
[0037] S3. Seed culture: use glucose 3.0%, KH2PO40.15%, MgSO40.05%, FeSO410mg / L, MnSO410mg / L, corn steep liquor 2.5%, yeast powder 0.30%, VB12mg / L, amino acid powder 0.5%, bubble enemy 0.002%, put it into the seed medium, adjus...
Embodiment 2
[0042] When L-threonine is fermented to 16 hours, use the scraper concentration and evaporation system to carry out vacuum evaporation to remove acetic acid and NH4+ in the fermentation broth. The concentrated fermentation broth is pressed into the fermentation tank by a diaphragm pump to continue fermentation, and the evaporation is replenished with sterile water. After 16 hours of evaporation, the acetic acid in the coupling fermentation has been maintained at a low level in the middle and late stages of the fermentation. At the end of the fermentation, the acetic acid was 2.7g / L, while the fermentation acetic acid in the control group was 9.2g / L , the concentration of acetic acid decreased by 70.65%, and the concentration of ammonium ion decreased from 132.0mmol / L in the control fermentation to 52.9mmol / L in the coupled fermentation, a decrease of 59.92%. The amount of bacteria in the coupled fermentation maintained a rapid growth trend. At 36 hours of fermentation, the OD60...
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Abstract
The invention relates to the technical field of threonine fermentation, and discloses a method for improving the fermentation yield and conversion rate of threonine. The method for improving the fermentation yield and conversion rate of threonine comprises the following steps: S1, preparing materials; s2, slant culture; s3, seed culture; s4, fermentation culture; according to the method, fermentation liquid containing high-concentration acetic acid and ammonium ions in the middle and later periods of threonine fermentation enters a feed liquid inlet of the scraper evaporator from the fermentation tank under the dual action of the positive pressure of the fermentation tank and the negative pressure of the scraper evaporator by utilizing a fermentation coupling scraper concentration evaporation technology; acetic acid and ammonium ions enter the condenser through evaporation, concentrated fermentation liquor enters the fermentation tank through the concentrated liquor temporary storage tank for continuous fermentation, the process operation is simple, energy consumption is low, continuous feeding can be achieved, volatile substances such as acetic acid and ammonium ions can be continuously removed, continuous discharging can be achieved, the acetic acid and ammonium ion removal efficiency is high, and the production cost is low. The stability and the automation degree are high.
Description
technical field [0001] The invention belongs to the technical field of threonine fermentation, and specifically relates to a method for increasing the fermentation yield and conversion rate of threonine. Background technique [0002] The chemical name of threonine is α-amino-β-hydroxybutyric acid, which was isolated and identified by W.C. Rose in fibrin hydrolyzate in 1935. Among the eight essential amino acids required by humans and animals, threonine is the fourth amino acid after methionine, lysine, and threonine. Threonine plays an increasingly important role in human growth and development, and is widely used in food industry, breeding industry, and medical treatment. [0003] At present, Escherichia coli direct fermentation is the preferred technology for the industrial production of L-threonine. During the fermentation process, excessive accumulation of metabolic by-products such as acetic acid and NH4+ will have a serious impact on bacterial growth, bacterial viabil...
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