Supercharge Your Innovation With Domain-Expert AI Agents!

Biological nano hybrid system modified electrode as well as preparation method and application thereof

A bio-nano and bio-electrochemical technology, applied in the field of bio-nano hybrid system modified electrode and its preparation, can solve the problems of large energy consumption, complicated preparation process, environmental pollution, etc., and achieve the effect of improving the output power index

Pending Publication Date: 2022-05-10
NANJING UNIV OF SCI & TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above modification strategies all require physical / chemical pretreatment of the electrode in advance, the preparation process is complicated, energy consumption is large, it is easily affected by the operating environment, the cost is high, and the process will cause environmental pollution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological nano hybrid system modified electrode as well as preparation method and application thereof
  • Biological nano hybrid system modified electrode as well as preparation method and application thereof
  • Biological nano hybrid system modified electrode as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Prefabricate a solid plate containing 18g / L nutrient broth and 12g / L agar, inoculate the Shewanella species on the plate, cultivate it in a constant temperature incubator at 30°C for 48 hours, and inoculate a single colony on the plate into the LB culture medium (18g / L nutrient broth), shake and culture in a constant temperature shaking incubator at 30°C for 14 hours, and the bacterial solution is centrifuged and washed to harvest the precipitate;

[0036] (2) Prepare PBS buffer solutions with pH=7 and concentrations of 100, 50, 40, 30, 20, and 5 mM (calculated as total phosphorus), in which Na 2 HPO 4 12H 2 O and KH 2 PO 4 The addition amount is 24.811g / L and 4.18g / L, other concentrations are converted according to the gradient, and NaCl0.5g / L, NH 4 Cl1 g / L, MgSO 4 ·7H 2 O0.120 g / L, CaCl 2 0.011 g / L; prepare PIPEs buffer solution with pH=7, including piperazine-1,4-diethanesulfonic acid 30mmol / L, NaOH45 mmol / L, NaCl0.5g / L, NH 4 Cl1g / L, MgSO 4 ·7H 2 O0.120...

Embodiment 2

[0042] The bacterium liquid of harvest in embodiment 1 is diluted to OD with PIPEs buffer solution 600 =0.5, and ferric chloride, sodium thiosulfate and sodium formate shake culture under complete anaerobic conditions, the final concentration of ferric chloride is 0.55, 1.10, 1.65, 2.2, 2.75mM respectively, and the final concentration of sodium thiosulfate is 10mM , the final concentration of sodium formate was 120mM, and the shaking culture conditions were the same as in Example 1. The curves of the concentration of ferrous iron reduced with time in different ferric chloride final concentration systems are as follows: figure 2 As shown, it can be seen that the reduction of ferric iron in each group was completed within 48 hours, and the ferric chloride reduction rate significantly decreased after the final concentration of ferric chloride was greater than 2.2mM. When the concentration of ferric chloride was too high, the biological toxicity increased, so chlorination The fi...

Embodiment 3

[0044] The bacterium liquid of harvesting among the embodiment 1 is with PIPEs buffer solution, ferric chloride, sodium thiosulfate and sodium formate shaking culture under complete anaerobic condition, the final concentration of ferric chloride is 2mM, and sodium formate final concentration is 120mM, shaking culture The conditions are the same as in Example 1. The final concentration of sodium thiosulfate is 4, 10, 20, 40, 60, mM, preferably 10 mM. After 3 days of mixed culture, centrifuge to harvest the biological nano-hybrid product precipitation and perform the same operation as in Example 1 step (5) to obtain a powder sample. The SEM characterization results are as follows: image 3 It can be seen that above 10 mM, Shewanella tends to form more free FeS with poor activity, while the yield of nanoparticles will be affected below this concentration.

[0045] It can be seen that under this optimal condition, a large number of petal-shaped FeS nanoparticles are completely wra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biological nano hybrid system modified electrode as well as a preparation method and application thereof. The method comprises the following steps: firstly, constructing a single-chamber bioelectrochemical system, sequentially adding shewanella bacteria liquid, a PIPEs buffer solution with the pH value of 7.0 and an organic metabolic substrate, culturing and enriching a shewanella bacteria biological membrane at an anode at constant potential to prepare a biological membrane electrode, then transferring the biological membrane electrode into the PIPEs buffer solution, and carrying out electrochemical treatment on the PIPEs to obtain the PIPEs / shewanella bacteria biomembrane. And sequentially adding a ferric chloride solution, a sodium thiosulfate solution and an organic metabolic substrate, and culturing in a constant-temperature shaking table to prepare the biological nano hybrid system modified electrode. The electrode modified by a biological nano hybrid system obviously enhances extracellular electron transfer of microorganisms, and the output power density is increased by 5 times in a typical bioelectrochemical system.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and relates to a bio-nano hybrid system modified electrode, a preparation method and application thereof. Background technique [0002] A bioelectrochemical system (BES) is an electrochemical system using biocatalysts at the anode and / or cathode, which can directly convert the chemical energy stored in a variety of biodegradable organic substances into electrical energy through microbial metabolism, and can also be improved by electrical energy input. Microbial intracellular metabolism produces valuable substances with wide applicability. There are many types of microorganisms that can be involved in the BES system and the types of organic pollutants used for energy supply. Compared with the traditional system, it also has the advantages of small size, low noise, and small environmental impact. However, with the deepening of research, the drawbacks of BES, which are limited by the elect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C02F3/00C02F3/34
CPCC02F3/005C02F3/34Y02E60/50
Inventor 陈丹金超沈锦优江心白刘晓东
Owner NANJING UNIV OF SCI & TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com