Low-temperature preservation diluent for semen of plectropomus leopardus, preservation method and application

Abstract

The invention discloses a low-temperature preservation diluent for semen of plectropomus leopardus, a preservation method and application, and belongs to the field of artificial breeding of plectropomus leopardus. The diluent comprises the following components in parts by weight: 3.5 parts of sodium chloride, 1.68 parts of sodium bicarbonate, 2 parts of potassium chloride, 2 parts of reduced glutathione, 0.44 part of glucose and 10 parts of fetal calf serum. And diluting the semen of the plectropomus leopardus and the diluent according to a dilution ratio of (1: 9)-(1: 99), and storing at 0-4 DEG C. According to the invention, a novel formula for preserving the semen of the plectropomus leopardus at the low temperature of 0-4 DEG C is confirmed and put forward for the first time, the formula has no toxic effect on sperms, the longest sperm preservation time can reach 72 hours, the fertilization rate and the hatching rate are still 71.54% and 61.84% after the sperms are preserved for 24 hours, and compared with fresh sperms, the sperm preservation time is effectively prolonged, the sperm motility rate is ensured, and the survival rate of the sperms is improved. The requirement of low-temperature short-term storage of sperms during artificial improved variety breeding of plectropomus leopardus can be met.

Description

technical field [0001] The invention relates to the field of artificial breeding of leopard echinacea, in particular to a low-temperature preservation diluent, preservation method and application of leopard echinacea semen. Background technique [0002] The reproductive method of fish is in vitro fertilization. The sperm are inactive in the testis or seminal plasma. Once they are discharged into the water, they will be immediately activated by capacitation, and then start to move, which has the ability to fertilize. Leopard spiny perch, commonly known as East Star Spot, is an important and valuable mariculture species in the southern coast. In order to maximize profits, many aquaculture factories gradually increase their breeding density, which leads to the deepening of inbreeding and further exposes problems such as germplasm degradation and diseases. . Effective preservation of isolated sperm can create conditions for artificial insemination or even artificial breeding. ...

AI Technical Summary

Problems solved by technology

The appropriate dilution factor also affects the storage time. When the dilution factor is too small, the concentration of semen will be too high and viscous, resulting in low viability;

[0003] So far, there have been no reports or studies on the storage of leopard echinacea sperm at low temperature (0-4°C), only found in Sun Yat-Sen University Meng Zining's team on "Leopard echinacea Cryopreservation Solution and Its Preservation Method" (patent No.: ZL201410473950.6), but it is suitable for cryopreservation and the operation is relatively complicated. Generally, farms do not always have liquid nitrogen and liquid nitrogen tanks, and there are differences in the operation of different personnel. Affect the effect of sperm cryopreservation. Even if some sperm are viable after thawing, their internal and external physiological and biochemical functions are damaged by freezing, and they do not have the ability to fertilize

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