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Method for fermenting food industry leftovers by fermenting agent containing bacillus velezensis

A technology for bacillus and fermented food, which is applied in the field of fermented food industry scraps containing bacillus velesi as a starter, can solve the problems of many residual toxins, high cost of raw materials, and large water pollution, and can inhibit or kill other The effect of miscellaneous bacteria, simple fermentation process and high economic value

Active Publication Date: 2022-05-13
HESHAN SEWETE BIOLOGICAL PROD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Artificial cultivation of living water spiders requires special bait. At present, common chicken manure and other manure fertilizers are mainly used to cultivate water spiders, and some feed materials are used to prepare such as water spiders. The cost of raw materials is relatively high, so using leftovers from the food industry as raw materials for the production of special bait for water spiders has become a better choice for the development of special bait for water spiders
[0007] To ferment protein-containing waste and sugar-containing waste into special bait for cultivating water spiders, it is necessary to screen out special strains suitable for fermenting and decomposing these wastes. Currently, the commonly used strains of fermented feed are Bacillus subtilis, Saccharomyces cerevisiae, etc. , using these strains to ferment these leftovers is often over-fermented, and most of the crude protein is fermented into water-soluble amino acids or small peptides, which are not suitable for feeding water spiders, so a strain with a proper fermentation degree must be selected. to ferment the above leftovers to convert them into bait materials for water spiders

Method used

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  • Method for fermenting food industry leftovers by fermenting agent containing bacillus velezensis
  • Method for fermenting food industry leftovers by fermenting agent containing bacillus velezensis
  • Method for fermenting food industry leftovers by fermenting agent containing bacillus velezensis

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Experimental program
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Effect test

Embodiment 1

[0030] Screening of Bacillus Velez XDY021: 4 strains of Bacillus (XD05B-XD08B), 1 strain of yeast (XD05J) were purchased from the Culture Collection Center of Guangdong Institute of Microbiology, and 2 strains of yeast (XD06J) were purchased from Guangzhou Baijia Supermarket. -XD07J), and then isolated and screened 4 strains of bacillus (XDY020-XDY023) and 3 strains of yeast (XD05J-XD07J) from nature, and then they were subjected to fermentation substrates composed of 3 generations of protein-containing waste and sugar-containing waste. Finally, the strains that can ferment the above-mentioned fermentation substrates into raw materials for cultivating water spiders are selected.

[0031] The above-mentioned protein-containing scraps include: yuba slag from Hecheng South Yuba Factory in Heshan City, Jiangmen City, Guangdong Province, soy sauce residue from Donggu Soy Sauce Factory in Heshan City, Jiangmen City, Guangdong Province, peanut bran from Taoyuan Peanut Oil Factory in H...

Embodiment 2

[0055] 1. The preparation of the starter containing Bacillus Veles: get the XDY021 bacterial classification and inoculate it into the culture medium sterilized by high temperature, and carry out fermentation and cultivation in a fermenter to get the starter containing Bacillus Veles, and also It is the fermented liquid of water spider bait raw material prepared by Bacillus Veles, and it is ready for use.

[0056] Specifically, the preparation steps are as follows:

[0057] (1) Preparation of XDY021 bacterial liquid: Inoculate the fresh culture of Bacillus velezensis XDY021 into 16 500ml Erlenmeyer flasks equipped with culture medium, cultivate in a water bath shaker at 35°C for 48h, and the stirring speed is 20-50rpm. The medium in this step is the same as that in step (2).

[0058] (2) Preparation of medium: use glucose, peptone, yeast extract, KH 2 PO 4 , sodium chloride, manganese sulfate and water are the main raw material preparation medium, and their mass percentages...

Embodiment 3

[0070] 1. The preparation of the starter containing Bacillus Veles: inoculate the XDY021 strain into the high-temperature sterilized medium, and carry out fermentation and cultivation in a fermenter to obtain the starter containing Bacillus Velez, which is ready for use .

[0071] Specifically, the preparation steps are as follows:

[0072] (1) Preparation of XDY021 bacterial liquid: inoculate fresh culture of Bacillus velezensis XDY021 into 16 500ml Erlenmeyer flasks filled with culture medium, and incubate in a biochemical incubator at 38°C for 48 hours. The medium in this step is the same as that in step (2).

[0073] (2) Preparation of medium: use glucose, peptone, yeast extract, KH 2 PO 4 , sodium chloride, manganese sulfate and water are the main raw material preparation medium, and their mass percentages are:

[0074] Peptone 3%, yeast extract 2.5%, glucose 2%, KH 2 PO 4 0.5%, sodium chloride 0.4%, manganese sulfate 0.04%, water balance.

[0075] (3) Inoculate t...

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Abstract

The invention discloses a method for fermenting food industry leftovers by using a fermentation agent containing bacillus velezensis, which comprises the following steps: (1) inoculating bacillus velezensis XDY021 bacterial liquid into a sterilized culture medium, carrying out shake-flask culture, and then transferring into a seed tank for culture to obtain the fermentation agent containing bacillus velezensis; (2) taking a fermentation substrate composed of protein-containing leftovers and sugar-containing leftovers, adding water, and grinding into slurry; and (3) putting the pasty fermentation substrate into a fermentation tank, adjusting the pH value, adding a fermentation agent containing bacillus velezensis, and fermenting for 24-120 hours, thereby obtaining the product. According to the method disclosed by the invention, the protein-containing leftovers and the sugar-containing leftovers are fermented by the fermentation agent containing bacillus velezensis, the particle size, the water insoluble content and the like of the obtained fermentation material just meet the requirements for culturing the spider, and the fermentation material is beneficial to ingestion of the spider and is particularly suitable for being used as a bait for culturing the spider. In addition, the bacillus velezensis XDY021 still exists, so that the bacillus velezensis XDY021 is resistant to storage, other infectious microbes cannot be bred in the storage process, and the phenomena of flatulence, expansion and the like cannot occur.

Description

technical field [0001] The invention relates to the application of Bacillus Velez, in particular to a method for fermenting food industry waste with a starter containing Bacillus Velez. Background technique [0002] The yuba factory and soy sauce factory use soybeans to produce yuba and soy sauce, which will produce a large amount of waste yuba residue and waste soy sauce residue (hereinafter collectively referred to as protein-containing scraps). These protein-containing scraps have always been used to feed fish ponds as a breeding Auxiliary bait for fish, because these leftovers have not been decomposed and can only be used in small quantities, their nutrient utilization rate is very low, and their added value is very low; on the other hand, because these leftovers are easy to contaminate bacteria , directly used as feed without sterilization and fermentation treatment, it is easy to cause disease in fish; there are many such protein-containing wastes, such as cakes and br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K10/38A23K50/90A23L5/20
CPCA23K10/12A23K10/37A23K10/38A23K50/90A23L5/28Y02P60/87
Inventor 杨雄易旻李少珍张丛光陈永红杨伊帆张其辉邓林英宋建亮
Owner HESHAN SEWETE BIOLOGICAL PROD
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