Method for fermenting food industry leftovers by fermenting agent containing bacillus velezensis
A technology for bacillus and fermented food, which is applied in the field of fermented food industry scraps containing bacillus velesi as a starter, can solve the problems of many residual toxins, high cost of raw materials, and large water pollution, and can inhibit or kill other The effect of miscellaneous bacteria, simple fermentation process and high economic value
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Embodiment 1
[0030] Screening of Bacillus Velez XDY021: 4 strains of Bacillus (XD05B-XD08B), 1 strain of yeast (XD05J) were purchased from the Culture Collection Center of Guangdong Institute of Microbiology, and 2 strains of yeast (XD06J) were purchased from Guangzhou Baijia Supermarket. -XD07J), and then isolated and screened 4 strains of bacillus (XDY020-XDY023) and 3 strains of yeast (XD05J-XD07J) from nature, and then they were subjected to fermentation substrates composed of 3 generations of protein-containing waste and sugar-containing waste. Finally, the strains that can ferment the above-mentioned fermentation substrates into raw materials for cultivating water spiders are selected.
[0031] The above-mentioned protein-containing scraps include: yuba slag from Hecheng South Yuba Factory in Heshan City, Jiangmen City, Guangdong Province, soy sauce residue from Donggu Soy Sauce Factory in Heshan City, Jiangmen City, Guangdong Province, peanut bran from Taoyuan Peanut Oil Factory in H...
Embodiment 2
[0055] 1. The preparation of the starter containing Bacillus Veles: get the XDY021 bacterial classification and inoculate it into the culture medium sterilized by high temperature, and carry out fermentation and cultivation in a fermenter to get the starter containing Bacillus Veles, and also It is the fermented liquid of water spider bait raw material prepared by Bacillus Veles, and it is ready for use.
[0056] Specifically, the preparation steps are as follows:
[0057] (1) Preparation of XDY021 bacterial liquid: Inoculate the fresh culture of Bacillus velezensis XDY021 into 16 500ml Erlenmeyer flasks equipped with culture medium, cultivate in a water bath shaker at 35°C for 48h, and the stirring speed is 20-50rpm. The medium in this step is the same as that in step (2).
[0058] (2) Preparation of medium: use glucose, peptone, yeast extract, KH 2 PO 4 , sodium chloride, manganese sulfate and water are the main raw material preparation medium, and their mass percentages...
Embodiment 3
[0070] 1. The preparation of the starter containing Bacillus Veles: inoculate the XDY021 strain into the high-temperature sterilized medium, and carry out fermentation and cultivation in a fermenter to obtain the starter containing Bacillus Velez, which is ready for use .
[0071] Specifically, the preparation steps are as follows:
[0072] (1) Preparation of XDY021 bacterial liquid: inoculate fresh culture of Bacillus velezensis XDY021 into 16 500ml Erlenmeyer flasks filled with culture medium, and incubate in a biochemical incubator at 38°C for 48 hours. The medium in this step is the same as that in step (2).
[0073] (2) Preparation of medium: use glucose, peptone, yeast extract, KH 2 PO 4 , sodium chloride, manganese sulfate and water are the main raw material preparation medium, and their mass percentages are:
[0074] Peptone 3%, yeast extract 2.5%, glucose 2%, KH 2 PO 4 0.5%, sodium chloride 0.4%, manganese sulfate 0.04%, water balance.
[0075] (3) Inoculate t...
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