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Trichoderma strain with wood preservation and biocontrol functions and application thereof

A technology of wood preservation and bacterial strains, which is applied in the field of biocontrol bacteria Trichoderma viridans strains, can solve the problems of high application cost, human and environmental damage, corrosion of metal connectors, etc., and achieve fast growth and reproduction, wide antibacterial spectrum, and environmental friendliness Effect

Pending Publication Date: 2022-05-27
INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The decay of wood is often prevented by chemical methods. In the past, copper chromate arsenide (CCA) was mainly used as a domestic preservative. Since chromium and arsenic are harmful heavy metals, they will cause harm to the human body and the environment. At present, the United States, the European Union, Japan and other countries Prohibit and restrict the use of CCA to treat wood successively
Ammonia-soluble alkylamine copper (ACQ) treated wood has good anti-corrosion and termite-proof performance, and has excellent anti-loss performance, but the high application cost is an important reason that limits its use
Copper azole preservative is an environmentally friendly high-efficiency preservative. It is widely used in wood and bamboo, plywood and recombined wood. It has been produced and applied by many domestic enterprises. With darker color, the treated wood cannot maintain its original color, and it has a corrosive effect on the metal joints of the wooden structure, which limits the scope of use of such preservatives to a certain extent, and seeks a more environmentally friendly and ecological protection method appears to be particularly important

Method used

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  • Trichoderma strain with wood preservation and biocontrol functions and application thereof
  • Trichoderma strain with wood preservation and biocontrol functions and application thereof
  • Trichoderma strain with wood preservation and biocontrol functions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Isolation, purification and identification of Trichoderma virens NY45 strain

[0026] 1) Screening of Trichoderma strains

[0027] Investigate the wooden water tank unearthed from the Nanyue National Palace Site, record and photograph the suspected fungal growth symptoms one by one, and use tweezers to pick up a small amount of wood tissue with suspected tissue; pick a small amount of wood tissue on a sterile operating table and soak it in the concentration The surface was sterilized in 0.1% mercuric chloride for 3 to 5 minutes, rinsed with sterile water for 3 times, and then transferred to a 2% agar medium containing 50 μg·mL-1 ampicillin for culture. After the colony grows on the agar medium, pick a single mycelium along the edge of the colony and cultivate it on the PDA medium to obtain a purified strain.

[0028] A fast-growing fungus was found in the obtained pure strain, which could fill the whole dish in three days, and was named NY45.

[0029] 2), t...

Embodiment 2

[0041] Example 2. Biological characteristics of Trichoderma virens NY45 strain

[0042] Malt juice agar medium (MEA): malt extract powder 20g / L, peptone 1g / L, glucose 20g / L, agar 15g / L; yeast extract agar medium (CYA): K2HPO4 1g, Chardonnay concentrate (NaNO3 300g / L, KCL 50g / L, MgSO4 7H2O 50g / L, FeSO4 7H2O 1g / L, ZnSO4 7H2O 1g / L, CuSO4 5H20 0.5g / L) 10mL / L, yeast extract 5g / L, sucrose 30g / L, agar 15g / L; 25% glycerol nitrate agar medium (G25N): K2HPO 40.75g / L, Char’s concentrate 7.5mL / L, yeast extract powder 3.7g / L, glycerol ( Analytical pure) 250g / L, agar 15g / L; Creatine agar medium (CREA): sarcosine 3g / L, sucrose 30g / L, KCL 0.5g / L, MgSO4 7H2O 0.5g / L, FeSO4 7H2O 0.01g / L, K2HPO4·3H2O or K3PO4·7H2O 1.3g / L, bromocresol violet 0.05g / L, agar 15g / L. The above media were all sterilized at 121 °C for 20 min.

[0043] Trichoderma virens NY45 CGMCC No.40004 was activated in a petri dish containing PDA medium, and after culturing for 3 to 7 days, the bacteria cake was punched along the...

Embodiment 3

[0048] Example 3. Test of the antagonistic effect of Trichoderma virens NY45 CGMCC No.40004 on various pathogenic bacteria

[0049] 1), the confrontation test of Trichoderma virens NY45 CGMCC No.40004 to wood decay fungus

[0050] The white rot fungus Trametes versicolor (L.) Lloyd and the brown rot fungus Gloeophylum trabeum (Pers.) Murrill) were activated in PDA medium, and after culturing for 3 to 7 days, they were treated with a diameter of For a 4mm hole puncher, punch the bacteria cake along the outer edge of the colony, and inoculate the Trichoderma virens NY45 CGMCC No.40004YN4-5 bacteria cake together with other bacteria cakes in a 90mm diameter PDA medium plate, and two. The strains were separated by about 40mm and about 20mm from the edge of the medium. The control group was inoculated with the bacterial cake alone in the center of another PDA medium plate, repeated 3 plates each, and placed in an incubator for cultivation. From the second day, the growth situation ...

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Abstract

The invention discloses a trichoderma strain for inhibiting wood decay fungi and application of the trichoderma strain in wood preservation and biocontrol. The Trichoderma virens strain is Trichoderma virens NY45 CGMCC (China General Microbiological Culture Collection Center) No.40004, and the Trichoderma virens strain is a Trichoderma virens strain The strain can inhibit growth of wood white rot fungi and brown rot fungi, and wood can be prevented from being infected by decay fungi and degraded after being treated by the trichoderma strain spore liquid.

Description

technical field [0001] The invention relates to a biocontrol bacteria Trichoderma viridans strain capable of inhibiting wood decay and degradation and its application. Background technique [0002] Wood is a renewable, energy efficient, biodegradable and environmentally friendly product. With the continuous enhancement of people's awareness of environmental protection and sustainable development, the advantages of renewability and environmental friendliness of biomass materials such as wood have attracted increasing attention. [0003] However, wood is easily spoiled under the action of various biological factors. Wood degradation caused by white rot fungi and brown rot fungi is the main type of spoilage in wood utilization. Rot fungi secrete enzymes directly to digest wood, and brown rot is rot caused by brown rot fungi first attacking the carbohydrates of the cell wall. White rot is formed by the simultaneous attack of carbohydrates and lignin in the cell wall by white r...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00B27K5/00C12R1/885
CPCC12N1/14A01N63/38B27K5/00B27K2240/20Y02A50/30
Inventor 马星霞方旋熊怡心蒋明亮
Owner INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY
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