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Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant

A compound bacterial agent and biocontrol technology, applied in the directions of fungicides, bacteria, biocides, etc., can solve the problems of soil micro-ecological balance destruction, soil and water pollution, affecting crop yield and quality, etc., to promote plant growth and environmental protection. friendly effect

Active Publication Date: 2022-07-08
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]At present, soil-borne diseases such as botrytis cinerea, blight, powdery mildew, root rot, fusarium wilt, sclerotinia, blight, etc. of melons, etc. Botrytis cinerea, sclerotinia, verticillium wilt, root rot, fusarium wilt, blight, etc. of fruit, gray mold of beans, fusarium wilt, blight, root rot, bacterial blight, etc., and aphids Homoptera pests such as planthoppers, whiteflies, etc., coleopteran underground pests such as grubs have brought serious losses to agricultural production, seriously affecting the yield and quality of crops; with the continuous expansion of the cultivated area of ​​agricultural protection land and the increase of the multiple cropping index of crops increased, soil-borne fungal diseases are serious, and often a variety of diseases occur in combination, which has become a serious obstacle to agricultural production
[0004] However, most of the current seed coating agents are medium and high-toxic chemicals, which remain in the soil for a long time, causing soil and water pollution and soil micro-ecological balance Destruction, pest resistance, etc.

Method used

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  • Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant
  • Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant
  • Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Determination of antibacterial spectrum of Bacillus velesi ZLP-101

[0030] Bacillus velezensis ZLP-101, the preservation number is CGMCC No. 20130, and the preservation unit is the General Microbiology Center of the China Microorganism Culture Collection Management Committee. The address is: No. 1 Beichen West Road, Chaoyang District, Beijing, China No. 3 Institute of Microbiology, Chinese Academy of Sciences, preserved on July 3, 2020.

[0031] Select common plant fungal disease pathogens such as: potato dry rot, potato early blight, potato fusarium wilt, potato ring rot, pear black spot, tomato botrytis, pepper anthracnose, tomato root rot, cucumber botrytis, The antibacterial spectrum of strain Bacillus velesi ZLP-101 was determined by plate confrontation method using various plant pathogens such as cotton fusarium wilt, grape white rot, botrytis cinerea, apple rot, and tobacco scab as targets.

[0032] Table 1 Inhibitory effect of Bacillus velesi ZLP-...

Embodiment 2

[0036] Example 2 Determination of antibacterial spectrum of Bacillus amyloliquefaciens WS-8

[0037] Bacillus amyloliquefaciens WS-8, the preservation number is CGMCCNo.11787, the preservation unit is the General Microbiology Center of the China Microorganism Culture Collection Management Committee, and the address is: 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China No. Institute of Microbiology, Chinese Academy of Sciences.

[0038] Select common plant fungal disease pathogens such as: cucumber Botrytis cinerea, cotton fusarium wilt, pear black spot, cucumber anthracnose, cucumber scab, apple rot, pink single Rui, wheat scab, tomato early blight, Corn blight, Phytophthora capsicum, Tomato leaf mold, Fusarium oxysporum, Staphylococcus aureus, Escherichia coli, Aeromonas viridis, Aeromonas hydrophila, Edwardsiella blunt, Candida albicans , Bacillus subtilis C-3 and other 20 kinds of plant pathogens were used as targets, and the antibacterial spectrum of Bacillus ...

Embodiment 3

[0043] Embodiment 3 Determination of antibacterial spectrum of Bacillus subtilis BSD-2

[0044] Bacillus subtilis BSD-2, the preservation number is CGMCC No. 8440, and the preservation unit is the General Microbiology Center of the China Microorganism Culture Collection Management Committee. The address is: No. 1, Beichen West Road, Chaoyang District, Beijing, China. No. Institute of Microbiology, Chinese Academy of Sciences.

[0045] Select common plant fungal disease pathogens such as: cucumber Botrytis cinerea, cotton fusarium wilt, pear black spot, cucumber anthracnose, apple rot, wheat scab, tomato early blight, corn blight, pepper Phytophthora , Fusarium oxysporum, Staphylococcus aureus, Escherichia coli, Salmonella and other 13 kinds of plant pathogens were targeted, and the antibacterial spectrum of Bacillus subtilis BSD-2 was determined by plate confrontation method.

[0046]Table 3 Determination of bacteriostatic spectrum of Bacillus subtilis BSD-2 sterile filtrate ...

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Abstract

The invention relates to a complex microbial inoculant. The complex microbial inoculant comprises bacillus velezensis ZLP-101, bacillus subtilis BSD-2 and bacillus amyloliquefaciens WS-8, the preservation number of the bacillus velezensis ZLP-101 is CGMCC (China General Microbiological Culture Collection Center) No.20130; the preservation number of the bacillus amyloliquefaciens WS-8 is CGMCC (China General Microbiological Culture Collection Center) No.11787; the preservation number of the bacillus subtilis BSD-2 is CGMCC (China General Microbiological Culture Collection Center) No. 8440. The invention also relates to a biocontrol seed coating agent containing the complex microbial inoculant. The bactericidal composition is safe, efficient, pollution-free, harmless to human and environment-friendly, and can be used for preventing and treating plant diseases and insect pests, increasing the emergence rate of crop seeds and promoting plant growth.

Description

technical field [0001] The invention relates to a composite bacterial agent and a biocontrol seed coating agent comprising the composite bacterial agent. Background technique [0002] Seed coating agent, as the most convenient and cost-effective way to control seed-borne, soil-borne diseases and underground pests, has become one of the indispensable pesticide products in agricultural production. [0003] At present, soil-borne diseases such as gray mold, blight, powdery mildew, root rot, fusarium wilt, sclerotinia, and blight of melons, gray mold, sclerotinia, verticillium wilt, etc. Root rot, fusarium wilt, blight, etc., botrytis cinerea, fusarium wilt, blight, root rot, bacterial blight, etc., as well as aphids, planthoppers, whiteflies and other Homoptera pests, grubs and other elytra Underground pests cause serious losses to agricultural production and seriously affect the yield and quality of crops; with the continuous expansion of the cultivated area of ​​agricultural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P7/04A01P21/00C12R1/07C12R1/125
CPCC12N1/20A01N63/22A01N2300/00Y02A40/10
Inventor 张飞燕张丽萍王瑞文王雅娜刘洪伟王江平董尧坤刘秋玥赵雯雅
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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