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Bacillus velezensis and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of microorganisms, can solve the problems of less research and achieve the effects of wide acid-base and salt tolerance, good development and application prospects, and good ability to dissolve organic phosphorus

Active Publication Date: 2022-07-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research and development in the field of pesticide application mainly focus on species such as Bacillus subtilis, Bacillus thuringiensis, and Bacillus megaterium. Therefore, the industrialized development of Bacillus Velez and its application in promoting growth and disease resistance in the agricultural field need to be further promoted and strengthened.

Method used

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  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1. Screening and identification of Bacillus velesi LPL-410.7

[0071] 1. Screening of Bacillus velesi LPL-410.7

[0072] Using the plate confrontation method, the screened excellent test strains were investigated for their antagonistic effects on various plant pathogenic fungi. (5 mm in diameter) placed in the center of the PDA medium, and 5 μL of the bacterial suspension to be tested was inoculated on the four ends of the crossed lines at equal distances from the fungal cake, and the pathogenic bacteria were inoculated alone as a control, and cultivated at a constant temperature of 28°C.

[0073] The screening results are shown in Table 1:

[0074] Table 1 Inhibitory effect of tested strains on pathogenic fungi

[0075]

[0076] From the results of the antibacterial effects of multiple strains on different pathogenic fungi, the results are shown in Table 1. It can be seen from Table 1 that LPL-410.7 has a good inhibitory effect on Rhizoctonia solani, Fusa...

Embodiment 2

[0092] Example 2. Performance determination of Bacillus velesi LPL-410.7

[0093] Preparation of the test bacterial suspension: the strain to be cultured was placed in the LB liquid medium with an inoculum of 3% (v / w), and then centrifuged at 3000r / min for 5min after culturing on a shaker for 20h (180r / min, 37°C). , get the bacterial body, wash it twice with sterile water, adjust the concentration of bacterial liquid to 10 8 CFU / mL for use.

[0094] 1. Determination of Phosphorus Dissolving Ability of Bacillus velesi LPL-410.7

[0095] The bacterial suspension of the tested strain was inoculated on the inorganic phosphorus medium (PKO inorganic medium) and the organic phosphorus medium (Monkina organic medium), cultivated at 37 ° C for 3 days, and observed whether there was a transparent circle, and according to the transparent circle. The ratio of diameter (D) to colony diameter (d) (D / d) preliminarily determined the ability of the strain to dissolve phosphorus. The experi...

Embodiment 3

[0139] Example 3. Antibacterial mechanism of strain LPL-410.7 against Botrytis cinerea

[0140] 1) Mycelium formation

[0141] The Botrytis cinerea cake (diameter 5mm) cultivated at 28°C for 5 days was placed in the center of the PDA medium, and 5 μL of the bacterial suspension to be tested was inoculated on the four ends of the crossed lines equidistant from the fungal cake to inoculate the pathogenic bacteria alone. As a control, the cells were incubated at a constant temperature of 28°C. The mycelia of the experimental group and the control group were collected for use. Drop 1 drop of lactic acid carbolic acid cotton blue staining solution on the glass slide, pick a small amount of sporulated mold mycelium from the edge of mold colony with a sterile dissection needle (or small tweezers), and place it in 50% ethanol to wash The exfoliated spores were removed, placed in the stain solution on a glass slide, and the hyphae were carefully dispersed with a dissecting needle. C...

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Abstract

The invention discloses a bacillus velezensis strain and application thereof. The invention specifically discloses a bacillus velezensis LPL-410.7 CGMCC (China General Microbiological Culture Collection Center) No.24303 strain and an application of the bacillus velezensis LPL-410.7 strain in prevention and treatment of plant diseases caused by phytopathogens and / or promotion of plant growth of the bacillus velezensis LPL-410.7 strain and the application of the bacillus velezensis LPL-410.7 strain. The bacillus velezensis LPL-410.7 disclosed by the invention has good organic phosphorus dissolving capacity and certain potassium dissolving capacity, and has relatively wide acid-base and salt tolerance; the strain can inhibit the growth of various pathogenic bacteria, generate hydrolase (protease and cellulase) related to pathogenic bacteria inhibition, generate siderophores and plant hormones (heteroauxin, gibberellin and cytokinin) for promoting plant growth, and is a biocontrol bacterium with excellent comprehensive performance. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to a strain of Bacillus velesi and its application, in particular to a strain of Bacillus velesi with disease prevention and life-promoting activity and its application. Background technique [0002] In recent years, the deterioration of soil properties, ecological environment pollution, biodiversity destruction, and product quality decline caused by the use of chemical fertilizers have become more and more serious. Increased emissions of gases and loss of biodiversity, serious threats to livestock and human health, such as nitrogen (N) and phosphorus oxides (P 2 O 5 ) excess can lead to the formation of toxic nitrates in groundwater and eutrophication of surface water, seriously polluting the air, water and soil. High levels of phosphorus hinder plant uptake of micronutrients such as copper and zinc and lead to their spread to human food and animal feed. Therefore, it is of gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14A01G13/00A01G7/06A01B79/00A01N63/22A01P1/00A01P3/00A01P21/00C09K17/14C05F11/08C05G3/00C05G3/60C12R1/07
CPCC12N1/20C12N11/14A01G13/00A01G7/06A01B79/00A01N63/22C09K17/14C05F11/08C05G3/00C05G3/60
Inventor 李平兰康慎敏穆文强武瑞赟张世奇韩飞尚楠
Owner CHINA AGRI UNIV
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