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Tissue engineering bone based on placenta source filling dry cell and production thereof

A stromal stem cell and tissue engineering technology, which is applied in the field of tissue engineered bone based on placenta-derived mesenchymal stem cells and its manufacture, can solve the problems of lack of detailed methods for large-scale separation, purification and expansion of placenta-derived mesenchymal stem cells. , to achieve the effect of convenient clinical application, no immune rejection, and large harvest

Inactive Publication Date: 2006-03-15
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only sporadic literatures at home and abroad about the small amount of placenta-derived adherent cells, which are mainly used for hematopoietic support research, but there is a lack of detailed technical information on the large-scale separation, purification and expansion of placenta-derived mesenchymal stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 The acquisition of human placenta-derived mesenchymal stem cells

[0021] Immediately after delivery, the placenta is placed in a sterile tray or container, injected with balanced salt solution containing anticoagulants and antibiotics via the umbilical artery, exsanguinated, and perfused. The anticoagulant can be 1~100u / mL heparin, and the antibiotic can be penicillin and streptomycin. Collect the perfusate and centrifuge to obtain cell components; or aseptically cut the placental leaflets after the initial perfusion, digest the placental tissue with trypsin or collagenase to obtain a cell suspension, and then pass the cell suspension through a cell sieve to remove tissue debris and blood cells, The obtained cells were resuspended, centrifuged at 500g for 30 minutes with percoll with a density of 1.073g / mL, harvested the interfacial cell layer, washed with PBS and inoculated into culture bottles or culture plates, or directly used for other purposes. It is a...

Embodiment 2

[0022] Example 2 A method for manufacturing tissue-engineered bone based on placenta-derived mesenchymal stem cells

[0023] Freshly isolated or cryopreserved human placenta-derived mesenchymal stem cells were prepared into 1.5-2×10 7 / ml of cell suspension, inoculated onto the scaffold material, trying to make the cells evenly distributed. In a preferred solution, the scaffold material adopts cylindrical β-TCP. The β-TCP porous bioceramic material can be produced by the patented method and process of Shanghai Biolu Company, and the material is passed through 60gray 60 Co irradiated and sterilized for tissue engineered bone construction. In a preferred solution, its microstructure is: pore diameter -100-300 μm, porosity -52%, inter-pore connection diameter -30-100 μm. After standing still for 6 hours, the complexes were transferred into RCCS (rotary bioreactor, Synthecom Company), and cultured in DMEM containing 10% FCS for 48 hours at a rotation speed of 30 rpm. The cultur...

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Abstract

A tissue engineering cartilage based on placenta original filled stem cell and its production are disclosed. The process is carried out by constructing bony tissue from composite biological brace material of placenta original filled stem cell. It can be used to repair bony injure and bony tissue deficit.

Description

field of invention [0001] The invention belongs to the field of tissue engineering, in particular to a tissue-engineered bone based on placenta-derived mesenchymal stem cells and a manufacturing method thereof. Background of the invention [0002] Clinically, a variety of reasons often cause bone tissue lacunar defects, such as femoral head necrosis caused by aseptic inflammation, lacunae left after maxillary sinus tumor surgery, and alveolar sockets produced after extraction of teeth. There is no ideal method for the treatment of these defects. For femoral head necrosis, artificial joint replacement is usually performed after the cortical bone or articular surface collapses. The latter cannot achieve the physiological recovery of joint function and will cause many complications; Whether the flap is repaired or not, both will cause changes in facial shape, which is unacceptable to the patient, and even makes life worse than life; most of the alveolar sockets left after toot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N11/14A61F2/28C12N5/0735C12N5/0775
Inventor 郭希民王常勇周晓东段翠密江红董灵芝李晶
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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