Affinity vinyl cyanide base co-polymer superfine fibre film preparation method and application

An ultra-fine fiber, acrylonitrile-based technology is applied in the field of preparation of an affinity acrylonitrile-based copolymer ultra-fine fiber membrane, and achieves the effects of simple preparation, simple and feasible immobilization method, and improved immobilization rate

Inactive Publication Date: 2009-10-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Publication No. CN 1358783 discloses the technology of using 4-azidobenzoic acid bifunctional cross-linking agent to chemically bond chitosan on the surface of the substrate under the action of ultraviolet light. Whether the method is active to the substrate The group is not required, but the disadvantage is that it can cause the bulk crosslinking of chitosan itself

Method used

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  • Affinity vinyl cyanide base co-polymer superfine fibre film preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The acrylonitrile / allylamine copolymer (viscosity-average molecular weight is 80,000) that mass fraction is 4% is dissolved in the dimethylacetamide solvent (being the mass percent of acrylonitrile / allylamine copolymer in dimethylacetamide Concentration is 4%), under the condition that the spinning voltage is 20 kilovolts, the spinneret solution flow rate is 0.5 ml / hour, and the receiving distance is 15 centimeters, electrospinning is carried out to prepare acrylonitrile with a diameter of 98 ± 14 nanometers Base copolymer microfiber membrane.

[0024] Dissolve chitosan with a viscosity-average molecular weight of 7000 in an acetic acid-sodium acetate buffer solution of pH 5.0, stir at room temperature for 24 hours, and prepare a homogeneous transparent solution with a mass fraction of 5 mg / ml; at 25°C, add 0.1 10 milliliters of acrylonitrile-based copolymer microfiber membranes were immersed in 10 milliliters of pH in 6.0 volume fractions of 1% glutaraldehyde aqueous s...

Embodiment 2

[0026] The acrylonitrile / allylamine copolymer (viscosity-average molecular weight is 80,000) that mass fraction is 4% is dissolved in the dimethyl sulfoxide solvent, is 20 kilovolts at spinning voltage, spinneret solution flow rate is 0.5 ml / Electrospinning is carried out under the conditions of 1 hour and a receiving distance of 15 cm, and an acrylonitrile-based copolymer ultrafine fiber membrane with a diameter of 98 ± 14 nm is prepared.

[0027] Dissolve chitosan with a viscosity-average molecular weight of 7000 in acetic acid-sodium acetate buffer solution of pH 5.0, stir at room temperature for 24 hours, and form a homogeneous transparent solution with a mass fraction of 10 mg / ml; 0.1 gram of acrylonitrile-based copolymer microfiber membrane is immersed in 10 milliliters of pH and is 6.0 in the glutaraldehyde aqueous solution of 5% by volume fraction, activates 12 hours, and the fiber membrane after the activation uses a large amount of buffer solution (buffer with dissol...

Embodiment 3

[0029] The acrylonitrile / methallylamine copolymer (viscosity-average molecular weight is 240,000) that mass fraction is 5% is dissolved in dimethylformamide solvent, is 12 kilovolts at spinning voltage, spinneret solution flow rate is Electrospinning was carried out under the conditions of 1.0 ml / hour and a receiving distance of 15 cm to prepare an acrylonitrile-based copolymer ultrafine fiber membrane with a diameter of 190±20 nm.

[0030]Dissolve chitosan with a viscosity-average molecular weight of 7000 in an acetic acid-sodium acetate buffer solution of pH 5.0, stir at room temperature for 24 hours, and prepare a homogeneous transparent solution with a mass fraction of 5 mg / ml; at 25°C, add 0.1 10 milliliters of acrylonitrile-based copolymer microfiber membranes were immersed in 10 milliliters of pH in 6.0 volume fractions of 1% glutaraldehyde aqueous solution, and activated for 12 hours. Consistent) rinse, filter dry, then put into chitosan solution and react for 10 hours...

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Abstract

The invention discloses a preparation method of an affinity acrylonitrile-based copolymer superfine fiber membrane. The acrylonitrile-based copolymer is dissolved in a solvent to form a solution, and then electrospun to prepare a fibrous film. The mass percentage of the acrylonitrile-based copolymer in the solution is 2 to 8%, and the spinning voltage of the electrospinning is 6-25 kV, the flow rate of the spinneret solution is 0.5-2.0 ml / hour, and the receiving distance is 8-20 cm; the acrylonitrile-based copolymer is acrylonitrile / allylamine copolymer, acrylonitrile / methallylamine copolymer , acrylonitrile / acrylamide copolymer or acrylonitrile / methacrylamide copolymer, with a viscosity average molecular weight of 80,000 to 300,000; the solvent is dimethylsulfoxide, dimethylformamide or dimethylacetamide One type or multiple types mixed in any ratio. After the chitosan is fixed by chemical bonds on the surface of the membrane, it is used for separating and purifying proteins, and has simple operation, good chemical stability and low cost.

Description

technical field [0001] The invention relates to a preparation method and application of a fiber membrane, in particular to a preparation method and application of an affinity acrylonitrile-based copolymer superfine fiber membrane. Background technique [0002] Chitosan is the deacetylation product of chitin. Due to its good biocompatibility, biodegradability and antibacterial properties, it has a wide range of applications in the fields of biomedicine, pharmacy, food, agriculture, and wastewater treatment. A large number of amino groups that exist on chitosan make it easy to combine with ligands. It is a good affinity material and can be applied to affinity separation (such as US Patent 4970001), separation and purification of proteins (such as US Patent US 5993661), immobilization of cells and enzymes (such as the Chinese patent literature with publication number CN 1285403 and US Patent US 4094743) and the like. However, chitosan is a kind of alkaline polysaccharide, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D71/40B01D69/08B01D71/82
Inventor 徐志康车爱馥黄小军
Owner ZHEJIANG UNIV
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