A method of selectively producing male or female sterile plants

A plant and male technology, applied in the field of seeds, can solve the problem of non-routine production of hybrid seeds

Inactive Publication Date: 2009-11-11
SYNGENTA BIO TECH CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Despite these methods of producing conditionally sterile male and female parental lines, hybrid seed production is far from routine in crops such as wheat

Method used

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  • A method of selectively producing male or female sterile plants
  • A method of selectively producing male or female sterile plants
  • A method of selectively producing male or female sterile plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0155] Dependent on exogenous administration of D-phosphinothricin or D-alanine or D-leucine or D-methionine or D-asparagine D-aspartate or D-glutamate Conditionally female sterile tobacco plants

[0156] The DNA sequence encoding the D-amino acid oxidase protein sequence Q99042 (Swissprot) in the EMBL sequence Z50019 was obtained or synthesized from the mRNA of the variant Trigonopsis variabilis by RT-PCR. As an alternative, obtain from yeast-like Rhodosporidium tolruloides (Rhodotorula gracilis) mRNA by RT-PCR or obtain it synthetically (this makes it easier to control the presence of internal restriction enzyme sites and produce Flanking sites to facilitate cloning) the DNA sequence encoding the D-amino acid oxidase protein sequence P80324 (Swissprot) in the EMBL sequence A56901, which can be synthesized, for example, as SEQ ID #7, designed for use in plants (here is wheat) codon usage and used to minimize DNA features potentially detrimental to expression. Alternatively,...

Embodiment 2

[0165] Dependent on exogenous administration of D-phosphinothricin or D-alanine or D-leucine or D-methionine or D-asparagine or D-aspartic acid or D-glutamic acid Conditionally female sterile tobacco plants

[0166] The DNA sequence encoding the D-amino acid oxidase protein sequence Q9HGY3 (Sptrembl) in EMBL sequence AB042032 was obtained by RT-PCR from Candida boidini mRNA or by synthesis. As an alternative, the DNA sequence encoding the D-amino acid oxidase protein sequence P24552 (Swissprot) in EMBL sequence D00809 was obtained by RT-PCR from Fusarium solani mRNA or by synthesis. Design flanking PCR primers or synthesize DNA sequences to place useful single restriction sites. Preferably and in cases where the oxidase coding sequence does not contain confounding internal sites, Nco1 and Nde1 sites are placed at the 5' end to facilitate cloning of in-frame fusions added to the 5' sequence of the ORF. Alternatively, when placing a restriction site upstream of the ATG transla...

Embodiment 3

[0176] A chimera that preferentially expresses and encodes enzymes capable of hydrolyzing imazamox or flamprop M methyl or flamprop M isopropyl into their respective carboxylic acids in male reproductive structures Gene

[0177] The DNA sequence encoding the carboxylesterase protein sequence Q01470 (Swissprot) in EMBL sequence M94965 was obtained by PCR from Arthrobacter oxydans genomic DNA or by synthesis. As an alternative, the DNA sequence encoding the carboxylesterase protein sequence P37967 (Swissprot) in the EMBL sequence BS06089 was obtained by PCR from Bacillus subtilis genomic DNA or synthesized. Alternatively, the DNA sequence encoding the carboxylesterase protein sequence P40363 (Swissprot) in EMBL sequence Z34288 was obtained from Saccharomyces cervisiae mRNA by RT-PCR or synthesized. Flanking PCR primers or synthetic DNA sequences are designed to place useful single restriction sites for cloning. Preferably and in cases where the carboxylesterase coding sequence...

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Abstract

A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and / or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is selected from the group consisting of ester derivatives of non-phosphonate herbicides which herbicides are directly phytotoxic to non-green tissue, D-alpha amino acids, peptide derivatives of non-protein D-alpha amino acids, S-enantiomers of aryloxyphenoxypropionates and S-enantiomers of ester derivatives of aryloxyphcnoxypropionatcs and (ii) the enzyme is selected from the group consisting of carboxylesterases, D-amino acid oxidases, D-amino acid dehydrogenases, D-amino acid racemases, 2-arylpropionyl-CoA epimerases, alpha-methylacyl-CoA racemases, thioesterases and acyl-CoA synthetases.

Description

Background technique [0001] Heterosis in crop plants has a significant effect on yield enhancement. Generally, hybrids show increased yield compared to non-hybrid varieties. Hybrids generally give more return units to growth factors such as water and fertilizers. Hybrids often offer superior stress tolerance, yield and consistency of maturity, and also provide simple breeding opportunities to combine traits or traits that would otherwise be difficult to combine. Often, heterosis in plants is important enough to warrant commercial exploitation. Commercial hybrids are widely used in many crops including corn, sorghum, sugar beet, sunflower and canola. However, wheat, barley and rice have mainly been bred by inbreeding mainly due to the lack of methods for the production of economical hybrid seeds. [0002] Traditionally, hybrid seed production involves planting spaced apart plots of female and male parental lines, with only the female parent's seed harvested. To ensure that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N9/18C12N9/06C07F9/38C12P13/04C12P17/12A01H1/00A01N37/34A01N37/44A01N39/02A01N43/40A01N43/60A01N43/76A01N57/20A01N63/00C12N15/09C12P9/00C12P41/00
CPCC12N15/8287C12N9/0024C12N15/829C12P41/007C12P13/04C12N9/18C12N15/8289
Inventor T·R·豪克斯G·米歇尔S·T·哈德菲尔德P·A·汤普森R·维尼尔张雁
Owner SYNGENTA BIO TECH CHINA
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