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Boactivity peptide gene therapy method realized by propeptide recombination

A technology of bioactive peptides and expression methods, which is applied to peptide preparation methods, chemical instruments and methods, peptides, etc., can solve problems such as high cost, failure to meet industrial production requirements, and inability to realize the prevention and treatment of bioactive peptides. Prevent degradation, ensure recognition and effective cutting effect

Inactive Publication Date: 2010-02-17
BEIJING TAIHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention overcomes the problems existing in the prior art that cannot meet the requirements of industrialized production, is expensive, and cannot be used as a drug to enter the clinical simultaneous expression. Most of the products cannot realize the preventive and therapeutic effects of biologically active peptides.

Method used

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  • Boactivity peptide gene therapy method realized by propeptide recombination
  • Boactivity peptide gene therapy method realized by propeptide recombination
  • Boactivity peptide gene therapy method realized by propeptide recombination

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Construction of recombinant propeptide of anti-tumor angiogenesis peptide Anginex and its gene therapy

[0031] (1) Determining the sequence of the biologically active peptide: XXXXXX in the figure represents the amino acid sequence of the selected biologically active peptide, and each X represents an amino acid residue. According to the desired biological effect, users have selected peptides with specific biological activities from published literature or through their own previous research findings, such as promoting apoptosis and inhibiting tumor angiogenesis. Peptides, antiviral infection peptides that antagonize HIV or HCV invading target cell receptors, aptamer peptides that truncate intracellular signal transduction, and nutritional peptides that activate cellular functions to treat senile degenerative diseases. The mechanisms and principles of action of these bioactive peptides vary widely, and the subcellular locations where they act are also diverse...

Embodiment 2

[0106] Example 2, Construction of TAT-VHL β structural propeptide and anti-renal cancer effect:

[0107] Step 1: Determine the sequence of bioactive peptides: Renal cell carcinoma is a common malignant tumor, recurrence and metastasis after surgery are common, and the tumor is not sensitive to radiation and chemical anticancer drug treatment, so the prognosis of the patient Difference. Recent biological studies of renal cancer cells revealed that the proliferation and metastasis of these cells depend on insulin-like growth factor 1 (insulin-like growth factor 1, ILGF1) receptor signaling. Von Hippel-Lindau (VHL) is a tumor suppressor gene. Its expression product inhibits the ILGF1 receptor signaling through binding with protein kinase Cδ to induce apoptosis of renal cancer cells, and has a therapeutic effect on renal cancer. However, there are technical difficulties in the use of VHL protein in the treatment of renal cancer solid tumors. One is that the protein molecules with...

Embodiment 3

[0133] Example 3, Construction of recombinant adeno-associated virus expressing humanin propeptide against senile dementia and its protective effect on neurons.

[0134] Alzheimer's disease (AD) is a common degenerative disease of the central nervous system in the elderly. The etiology is currently unknown, but the pathology is characterized by neuronal loss, senile plaque formation, and neurofibrillary tangles. Using molecular biology screening technology, a 24 amino acid residue polypeptide-Humanin was found to antagonize AD pathological changes caused by various known genetic causes, especially neural apoptosis. We used this patented technology to construct a recombinant propeptide expressing Humanin, express the propeptide using an adeno-associated virus vector, and transduce rat cortical nerve cells, successfully preventing amyloid peptide (the most important pro-neuron apoptosis in AD occurrence). apoptosis induced by apoptosis.

[0135] Step 1: Determine the sequence ...

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Abstract

The invention discloses a gene treating method of biological active peptide through reconstructing front-peptide in the biological technological domain, which comprises the following steps: (1) affirming sequence of biological active peptide, (2) loading identification sequence of signal peptide enzyme at amino end of object peptide, (3) deviating cDNA code according to object peptide sequence, (4) synthesizing cDNA, (5) plugging double-chain cDNA segment with peptide-terminal and interior contact enzyme identification point to prepare expressive box.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a recombinant expression method for realizing biologically active peptides through recombinant propeptides. Background technique: [0002] Bioactive peptides are protein domains and functional region fragments. Compared with the parent protein molecule, it has the characteristics of small molecular weight, simple structure and specific function, and some biological functions of some peptides are greater than that of their parent proteins. It has important application value in diagnosis and treatment of diseases. How to obtain bioactive peptides is an important topic in peptide research and use. From the current scientific and technological level, there are the following methods for obtaining bioactive peptides: [0003] 1. Extracting bioactive peptides from animal and plant tissues rich in bioactive peptides: This is also the earliest method to obtain bioactive peptides. However, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00C07K1/00C07K14/00
Inventor 杨广孝
Owner BEIJING TAIHE BIOTECH CO LTD
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