Method for preparing fructooligosaccharide from hydrolyzing jerusalem artichoke by column type ion exchanging process

An ion exchange method and fructooligosaccharide technology, applied in the field of fructooligosaccharide preparation, can solve the problems of FOS quality affected, high production and purification costs, and low fermentation enzyme activity, so as to reduce management costs, equipment investment, and production costs. The effect of low cost and short process flow

Inactive Publication Date: 2007-09-19
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product FOS has low purity, the highest content is 55-60% (DW) (Wang Jianhua, 2003, Review of microbial fructo-oligosaccharide synthase and its application research, natural product research and development, Vol.15, No.6, 544-551.) , so that the FOS quality is affected
Considering the production of high-purity FOS, people have found another way to use polyfructose-rich plant materials such as Jerusalem artichoke as raw materials, and use microorganisms to produce polyfructose-degrading enzymes to produce fructo-oligosaccharides. Fermentation enzyme activity is not high, production and purification costs are high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Fresh Jerusalem artichoke, treated with 5% NaOH at 95°C for 30s, rinsed and peeled with water, added double the volume of water, boiled at 90°C for 1 hour, treated with Ca(OH) 2 processing, and pass into the CO 2 To neutrality, the precipitate is centrifugally filtered, and the filtrate is 3 / h passed through DOOLSS macroporous strongly acidic styrene-based cation exchange resin (Nankai University), the liquid was placed at room temperature for 2 hours, and the super strong anion exchange resin D280 (Nankai University), the juice was spray-dried.

Embodiment 2

[0032] Embodiment 2: the determination of material-water ratio

[0033] Weigh 4 portions of 100g fresh Jerusalem artichoke, wash, slice, add 0.5 times, 1 times, 1.5 times, 2 times the volume of water and boil at 80°C for 1 hour, take four samples of the boiled extract and wash them with 0.2M HCLO 4 Hydrolyze for 2 hours, use the DNS method to measure the content of reducing sugar in the acid solution to calculate the content of total sugar in the boiled extract, add 0.5 times of water to 100g Jerusalem artichoke to extract the total sugar extraction rate of 65%, 1 times the total sugar extraction rate of boiled extract 80.0%, 1.5 times boiled extract total sugar extraction rate 80.2%, 2 times boiled extract total sugar extraction rate 80.8%. Adding 100g of Jerusalem artichoke to 2 times the volume of water and boiling for 1 hour at 80°C resulted in a higher extraction rate of total sugar. However, adding one volume of water or continuing to increase the volume of water had lit...

Embodiment 3

[0034] Embodiment 3: Determination of boiling temperature

[0035] Weigh 4 portions of 100g fresh Jerusalem artichoke, wash, slice, add to them respectively, boil and extract with 1 volume of water at 80°C, 85°C, 90°C, 95°C for 1 hour. Take four samples of the boiled extract and pass through 0.2M HCLO 4 Hydrolysis 2h. The content of reducing sugar in the acid hydrolyzate was determined by DNS method to calculate the content of total sugar in the cooking extract. Add 1 volume of water to 100g Jerusalem artichoke and cook at 80°C to extract 80.0% of the total sugar, 82.8% at 85°C, 91.0% at 90°C, and 91.0% at 95°C The rate is 91.2%. It was found that extraction at 90°C for 1 hour resulted in a higher extraction rate of total sugar, and the increase of extraction temperature would not significantly increase the content of total sugar in the extraction solution. However, the boiled extract is brown in color.

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Abstract

The invention relates to a preparation method for fructooligosaccharid, specifically of hydrolyzing American artichoke to prepare fructooligosaccharid by means of column ion-exchange treatment, including: 1) preparing the American artichoke liquid; 2) treating the prepared extract with calcium hydroxide, airing the CO2 gas, precipitation centrifugal filtering to get transparent American artichoke liquid; 3) treating the American artichoke extract with cation exchange resin controlling the velocity of flow at 800-1200cm3 / h, discharge liquid pH value between 1.0-2.2; 4) dispositing the discharge liquid in room temperature for acidolysis 2-6 hours, passing through strong anion resin, concentrating the liquid, spray drying or drying in vacuum. The invention removes the impurity in the chicory by ion exchange resin, simultaneously reduces its pH value, controls the acid leaching time. Compared to the enzyme method, the present method can get a comparative product in purity, but a lower production cost. The method paves a foundation in the large-scaled for fructooligosaccharid with a simple craft, timesaving and powersaving.

Description

technical field [0001] The invention relates to the preparation of fructo-oligosaccharides, in particular to a method for producing fructo-oligosaccharides by using Jerusalem artichoke as a raw material and using an ion exchange column. Background technique [0002] Fructooligosaccharide (FOS), also known as fructooligosaccharide or sucrose trisaccharide oligosaccharide, is a natural active ingredient present in fruits, vegetables, honey and other substances, and is an excellent water-soluble dietary fiber. At present, fructooligosaccharide has become a widely popular functional food base material in the international food market in the past ten years due to its superior physiological functions. new sugar source. [0003] Physiological functions of fructooligosaccharides: [0004] a.. Two-way regulation of the flora in the body: promote the rapid proliferation of bifidobacteria, inhibit the reproduction of exogenous pathogenic bacteria and intestinal spoilage bacteria, and...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/08
Inventor 杜昱光胡建恩曹海龙赵静玫李曙光白雪芳刘晗
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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