Composition containing cistanche tubulosa and its extract for treating osteoporosis

A technology for Cistanche deserticola and osteoporosis, which can be used in drug combinations, medical preparations containing active ingredients, bone diseases, etc., and can solve problems such as large side effects, long medication time, and high drug prices

Inactive Publication Date: 2008-03-19
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of a clear understanding of the etiology of OP in Western medicine, the use of medication is not strong, and there are problems such as long medication time, large side effects, many contraindications, and high drug prices. Now people are turning their attention to natural Chinese medicines. Effective drugs for treating osteoporosis, monomer components and their effective parts

Method used

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  • Composition containing cistanche tubulosa and its extract for treating osteoporosis
  • Composition containing cistanche tubulosa and its extract for treating osteoporosis
  • Composition containing cistanche tubulosa and its extract for treating osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of extract or powder of Cistanche cistanche

[0020] 1. Take 10,000g of Cistanche tuberosum medicinal material (through a 10-mesh sieve), add 10 times the amount of water (according to the weight of the medicinal material), soak first, then decoct for 2 hours, and filter to obtain the water extract; the medicinal residue is extracted according to the above conditions Extract two more times. The three medicinal solutions were combined, concentrated and then dried under reduced pressure to obtain 5600 g of dark brown powdery aqueous extract of Cistanche cistanche.

[0021] 2. Take 10000g of Cistanche tubulosa medicinal material (through a 10-mesh sieve), add 10 times the amount (by weight of the medicinal material) of 60% ethanol, after soaking, reflux extraction for 2 hours, and filter to obtain the extract; the dregs are extracted according to the above conditions Extract twice again, combine three medicinal liquids, recover ethanol under reduced pressure, ...

Embodiment 2

[0028] Preparation of refined Cistanche tubulosa extract (n-butanol extraction method)

[0029] 1. Get 100 grams of the dark brown powdery Cistanche cistanche water extract obtained in Example 1 (1), dissolve it in 200 milliliters of hot water, let it cool, and dissolve it with 100 milliliters of petroleum ether (b.p.60-90° C.) Extraction and degreasing, repeated extraction and degreasing 5 times, extracted the degreased aqueous solution with n-butanol, each time with 100 ml of n-butanol, repeated extraction 6 times, after the extracted n-butanol liquids were combined, steamed under reduced pressure in a water bath Dry to obtain 26.8 grams of refined Cistanche tubulosa extract (refined Cistanche tubulosa aqueous extract).

[0030] Determination of the content of echinacoside in the refined Cistanche tubulosa extract: HPLC analysis Determination conditions: Shimadzu LC-20AT liquid chromatograph, reversed phase column (Kromasil, KR100-5C18; 150 × 4.6mm); mobile phase is 0.1% Fo...

Embodiment 3

[0036] Preparation of refined Cistanche tubulosa extract (macroporous resin adsorption method)

[0037] 1. Get 10 grams of the brown powdery Cistanche cistanche ethanol extract obtained by extracting in Example 1 (2), and refine it with a D101 macroporous resin column (macroporous resin can also be DA201, DM301 or DS401,) according to conventional procedures To operate, first elute with 500 milliliters of water (the amount of water is equivalent to 4-7 times the column volume; the weight ratio of medicinal material to macroporous resin is approximately equal to 1:2). Use thin-layer chromatography to monitor column chromatography elution, thin-layer chromatography conditions: polyamide membrane is used as the adsorption material, 36% acetic acid aqueous solution is used as the developing agent, the spread distance is 6cm, the Rf value is about 0.6-0.7, and the detection component is pine Fruit glucoside. During water elution, the degree of echinacoside not appearing in the wat...

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Abstract

A drug combination for preventing and remedying the osteoporosis contains one of or a mixture of an extract of coarse cistanche tubulosa water and / or alcohol, the extract of fine cistanche tubulosa water and / or alcohol, the mixture of the cistanche tubulosa with an aurantiamarin part, the cistanche tubulosa powder or coneflower glycoside, and the experiment indicates that all of the components has the effects of prompting the osteoblast proliferation and obviously increasing the bone weight coefficient, the bone ash content, the width of bone trabecula and the content of bone phosphorus, wherein the effect of the mixture of the cistanche tubulosa with the aurantiamarin part is strongest.

Description

technical field [0001] The invention relates to a pharmaceutical composition for treating osteoporosis containing traditional Chinese medicine extracts, in particular to a pharmaceutical composition for treating osteoporosis containing Cistanche tubulosa or active ingredients or effective parts derived from Cistanche tubulosum. Background technique [0002] Osteoporosis (Osteoporasis, OP) refers to a systemic skeletal disease that reduces bone mass and damages the microstructure of bone tissue, resulting in increased bone fragility and increased risk of fracture. It is a common disease in the elderly and postmenopausal women. and frequently-occurring diseases. The treatment of Western medicine mainly uses bone resorption inhibitors (such as estrogen, progesterone, calcitonin, isopropoxyflavone, bisphosphonates and vitamins and their derivatives, etc.) and bone formation stimulators (such as fluorine preparations, androgen and anabolic hormone, parathyroid hormone and its fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/64A61P19/10A61K31/7048
Inventor 杨中林马志国
Owner CHINA PHARM UNIV
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