Method for extracting tea-polyphenol and separating monomer EGCG from tea

A technology of tea polyphenols and tea leaves, which is applied in the field of extracting tea polyphenols and separating monomer EGCG, which can solve the problems of complex operation process, difficult control of conditions, and easy pollution of chromatographic columns, and achieve simple process, low cost, and avoid extraction process Effect

Inactive Publication Date: 2008-07-23
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] So far, the extraction of tea polyphenols is mainly through organic solvent extraction, metal ion precipitation, resin adsorption, etc., but a large amount of use of organic solvents and metal ions has brought great safety hazards and environmental disasters to production and human beings. To obtain pure catechin monomers or other monomers, further purification is required on the basis of extracted and separated crude products. At present, various chromatographic separation techniques are most commonly used, including column chromatography separation method, high performance liquid phase preparation process, High-speed countercurrent chromatography has the disadvantages of complex operation process, long cycle, difficult control conditions, huge total column load, low preparation effect, easy pollution of chromatographic column, short service life, etc.; and most of industrial production uses toxic solvents such as dichloromethane or Chloroform removes caffeine, which are unfavorable factors in the extraction process of tea polyphenols

Method used

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  • Method for extracting tea-polyphenol and separating monomer EGCG from tea
  • Method for extracting tea-polyphenol and separating monomer EGCG from tea
  • Method for extracting tea-polyphenol and separating monomer EGCG from tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The mass ratio of feeding is tea powder: solid-phase alkaline reagent is 1:0.1, wherein the solid-phase alkaline reagent is sodium bicarbonate.

[0036] At room temperature, first pulverize the tea leaves with a universal grinder, the particle size is less than 1mm, and add 0.5g (about 2.5mmol) of tea powder calculated according to the average molecular weight of 400 (estimated value) to the mortar, and 0.05g of sodium bicarbonate (0.6mmol), mechanical grinding, the grinding time is 30min, add 20ml of water, stir for 30min, centrifuge, take the supernatant, add phosphoric acid to adjust the pH value to 1~2, produce gray precipitate, filter to obtain gray solid 0.006g, get The yield was 1.2%, and the content was 95%. After the filtrate was dried, 0.085g of tea polyphenol crude product was obtained. The yield of tea polyphenol was 17%, and the content was 75%.

[0037] Qualitative and quantitative analysis of the product: using commercially available tea polyphenols as a ...

Embodiment 2

[0039] The feeding mass ratio is tea powder: solid-phase alkali reagent is 1:0.1, wherein the solid-phase alkali reagent is sodium bicarbonate. The added quality of tea powder is 0.5g.

[0040] Grinding time was 15min, other operations were the same as in Example 1, and 0.005g of gray solid was obtained with a yield of 1.0% and a content of 93%. After the filtrate was dried, 0.0825g of tea polyphenol crude product was obtained with a yield of 16.5%. was 73%. Product identification and content determination are the same as those described in Example 1.

Embodiment 3

[0042] The feeding mass ratio is tea powder: solid-phase alkali reagent is 1:0.1, wherein the solid-phase alkali reagent is sodium bicarbonate. The added quality of tea powder is 0.5g.

[0043] Grinding time is 5min, and other operations are the same as in Example 1 to obtain 0.004g of gray solid with a yield of 0.8% and a content of 95%. After the filtrate is dried, 0.0775g of tea polyphenol crude product is obtained with a yield of 15.5%. 70%. Product identification and content determination are the same as those described in Example 1.

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Abstract

The invention discloses a method of extracting tea polyphenol and separating monomer EGCG from tea, which is characterized in that: tea and solid phase alkali are mixed according to mass ratio of 1:0.1 to 2 and grinded jointly and mixed evenly before solved using water, stirred and centrifuged; inorganic acid is added into the supernatant to regulate pH value into 1.0 to 8.0, thereby getting sedimentation and filtering; filter residue is monomer EGCG; the filtrate is tea polyphenol solution which is then dried to get crude product of tea polyphenol. The extraction and separation method adopts the mechanical and chemical technique to extract tea polyphenol and separate monomer EGCG. The method of extracting tea polyphenol and separating monomer EGCG from tea has the advantages of new extraction and separation technique without solvent, simple technique, low cost, high yield, high product purity, short production period, no pollution basically and wide industrial prospect for extracting and separating.

Description

(1) Technical field [0001] The invention relates to a method for extracting tea polyphenols and separating monomer EGCG from tea leaves. (2) Background technology [0002] Tea polyphenols are a mixture of polyhydric phenol derivatives extracted from tea leaves, accounting for 13% to 30% of the dry weight of tea leaves, with catechins as the main component, accounting for 60% to 80% of the total phenolic content, mainly composed of Composition of epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), epicatechin gallate (EGCG), epicatechin gallate (ECG) (the structure is as follows ). [0003] [0004] R 1 = R 2 = H, catechin (C) [0005] R 1 =OH,R 2 = H, gallocatechin (GC) [0006] R 1 = H, R 2 =X, catechin gallate (CG) [0007] R 1 =OH,R 2 =X, gallocatechin gallate (GCG) [0008] Tea polyphenol is a kind of natural antioxidant, and it has been widely used in oil, food, medicine, daily chemical, light chemical, cosmetics, health care and many other asp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 王平苏为科陶春妙
Owner ZHEJIANG UNIV OF TECH
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