Preparation method of marine microorganism antifouling coating

A technology of marine microorganisms and antifouling coatings, applied in biochemical equipment and methods, antifouling/underwater coatings, microorganisms, etc., can solve problems such as genetic variation of marine organisms, death of seaweed and other organisms, and destruction of ecological balance, and achieve production Low cost, good anti-fouling effect, no environmental pollution effect

Inactive Publication Date: 2008-10-15
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because organic tin will cause serious pollution to the marine environment and cause genetic variation in marine organisms, the International Maritime Organization has passed a resolution requiring that organic tin be completely banned by 2008.
Polished cuprous oxide antifouling paint has become the mainstream of the e

Method used

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  • Preparation method of marine microorganism antifouling coating

Examples

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Example Embodiment

[0011] Example 1: Preparation of antifouling microorganisms

[0012] Pick a loop of the preserved antifouling microbial strain with an inoculation needle, and inoculate it into the seed medium (3g beef extract, 10g peptone, 5g sodium chloride, 1L sea water, pH 7.0-7.2). After 24 hours of incubation, press Inoculate 5% of the inoculation rate into a 250ml Erlenmeyer flask containing 100ml fermentation medium, culture at 150 rpm at 20°C for 48 hours; coarsely filter the fermentation broth with gauze; filter the mother liquor by centrifugation at 8000 rpm / min for 30 minutes, and the sediment is antifouling microorganism.

Example Embodiment

[0013] Example 2: Preparation of antifouling protease

[0014] The anti-fouling microbial fermentation broth prepared in Example 1 was centrifuged at 6000 rpm for 15 minutes, and the supernatant was taken out with ammonium sulfate with a saturation of 60% for salting out. After standing overnight, 8000 rpm / min for 30 minutes After centrifugation, the precipitate was collected, and then chromatographed with a Sephadex G-75 chromatography column. After collecting the same components, it was freeze-dried. The sample was subjected to SDS-polyacrylamide gel electrophoresis, and the molecular weight of the antifouling protease was approximately 65Kd.

Example Embodiment

[0015] Example 3: Preparation of microbial antifouling paint

[0016] Weigh 3.5 g of antifouling microorganisms in Example 1, add 15 g of rosin to 80 g of chlorinated rubber in xylene (40 / 100, W / W) solution and add 1.2 g of bentonite, and stir with an electric mixer for 10 minutes. Add 3.5g of filtered and centrifuged sediment (target active microorganism) and stir evenly to obtain microbial antifouling paint; apply the above-prepared paint on A3 steel, and hang the board in the sea for 40 days after the coating is completely dry , No sea creatures were seen on the surface of the hanging board.

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Abstract

The invention relates to a method for making a marine microorganism antifouling paint by a marine microorganism fermentation product. The method realizes that bio-material is utilized to prepare the antifouling paint which prevents halobios from being attached; the invention belonging to the biological fouling protection technical field comprises three processing steps of preparing antifouling microorganism, antifouling protease and microorganism antifouling paint; firstly, barnacle and mussel are treated to a homogenate; the homogenate is taken out and put on a liquid substrate agar plate; the antifouling microorganism is obtained by lineation, separation and cultivation; secondly, the microorganism is conducted to a liquid seed substrate by a bevel substrate and cultivated in a fermentation substrate; a target active matter antifouling protease is obtained through the treatments of decompression, refrigeration and drying; finally, rosin is added to an alloprene solution and evenly stirred, then swell soil is added to the mixed solution and evenly stirred by electromotion; the active matter antifouling protease is added and evenly mixed, thereby obtaining the microorganism antifouling paint. The method for making the marine microorganism antifouling paint by the marine microorganism fermentation product has simple process course, low production cost, good antifouling effect and no environmental pollution.

Description

Technical field: [0001] The invention relates to a method for preparing a marine microbial antifouling paint by applying a novel marine microbial fermentation product to the field of marine antifouling. The method can realize the preparation of an antifouling coating for preventing marine organisms from adhering by using biological raw materials, and belongs to the technical field of biological fouling protection. Background technique: [0002] There are a large number of fouling organisms in seawater, such as mussels, barnacles, and seaweeds. These marine organisms will attach to the ship's hull, seawater cooling piping, and even sonar. This phenomenon is collectively called marine biofouling. Marine biofouling will increase the navigation resistance of ships, reduce the heat transfer efficiency of seawater cooling systems, and cause sonar performance failures, which seriously affect the maneuverability and safety of ships. Therefore, it is of great significance to take me...

Claims

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Application Information

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IPC IPC(8): C09D5/16C12N1/00C12N9/50
Inventor 段东霞蔺存国郑纪勇
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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