Preparation method of anti-hemorrhoid extract containing rutin and naringin

A technology of naringin and extract is applied in the field of preparation of anti-hemorrhoid extract, can solve the problems of difficult product conformity, poor repeatability, large loss of components, etc., achieves easy molding and use, controllable process parameters, and improved Effects of Intrinsic Quality

Active Publication Date: 2009-04-01
SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rutin and naringin used in the direct mixing method are all pure products. The preparation process and purification of these two pure products through traditional Chinese medicine extraction are very complicated, the purification route is cumbersome, and the cost is very high; the cost of synthesis through western medicine is also very high. Large, the cost of preparing the anti-hemorrhoid composition will be relatively high
The composition prepared by direct water extraction or aqueous ethanol extraction will contain more other water-soluble components, such as protein, sugar, amino acid, etc., and some alkaloid components such as synephrine (can Excite a receptor, make blood pressure rise, and can strengthen the heart, anti-shock) and N-methyltyramine etc., these alkaloid components are non-flavonoids, non-anti-hemorrhoid components, will affect the anti-hemorrhoid composition purity
Also because there are many water-soluble components, the sample is sticky paste, the operation of concentration and drying is extremely troublesom...

Method used

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  • Preparation method of anti-hemorrhoid extract containing rutin and naringin
  • Preparation method of anti-hemorrhoid extract containing rutin and naringin
  • Preparation method of anti-hemorrhoid extract containing rutin and naringin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] [Example 1] The preparation method of the anti-hemorrhoid extract containing rutin and naringin. Extract the total flavonoids extract from the two medicinal materials

[0038] Take 100 kg each of the Chinese herbal medicine Fructus aurantii and Sophora japonica (or Sophora japonica), add 800 kg of 50% ethanol, heat and reflux for extraction for 3 hours, filter, add 700 kg of 50% ethanol for reflux to extract the medicinal residues for 2 hours, filter, and extract the medicinal residues Continue to add 600 kg of 50% ethanol, filter, combine the three extracts, recover the solvent under reduced pressure, and concentrate to 10 times the volume of the original medicinal material at a temperature of 45-80°C, dilute the concentrated solution with 3 times hot water, and add appropriate amount of traditional Chinese medicine to clarify Reagent ZTCA+B, centrifuge, remove the precipitate, take the supernatant, and load the sample on the D101 macroporous resin column (pre-treat th...

Embodiment 2

[0039] [Example 2] The preparation method of the anti-hemorrhoid extract containing rutin and naringin. Extract the total flavonoids extract from the two medicinal materials

[0040]Take 100 kg each of Chinese herbal medicine Fructus Aurantii and Sophora japonica (or Sophora japonica), add 1 ton of 85% ethanol, heat and reflux for 3 hours, filter, add 1 ton of ethanol for reflux and extract for 2 hours, filter, and extract twice liquid combined. Recover the solvent under reduced pressure, concentrate to 8 times the volume of the original medicinal material at a temperature of 45-80°C, dilute the concentrated solution with 5 times hot water, add an appropriate amount of traditional Chinese medicine clarifier ZTCA+B, centrifuge, remove the precipitate, take the supernatant, and put The sample was placed on a D101 macroporous resin column (the resin column was pretreated with acid, alkali, water, and alcohol before loading the sample), and after loading the sample was washed wit...

Embodiment 3

[0041] [Example 3] Quality Analysis of Anti-hemorrhoid Extract

[0042] Samples used in mass analysis include:

[0043] According to the method of Example 1, the anti-hemorrhoid total flavonoid extract prepared has 2 batch numbers in total.

[0044] According to the method of embodiment 2, the antihemorrhoid total flavonoid extract of preparation, totally 3 batch numbers

[0045] 1. Content analysis

[0046] 1.1 Determination of content of rutin and naringin

[0047] Using high performance liquid chromatography (HPLC), using rutin and naringin as reference substances, the content ratio of the two in the total flavonoid extract was determined to be 0.8-4:1.

[0048] 1.2.1 Chromatographic conditions

[0049] High performance liquid chromatography HP1100 series (U.S. Huipu Company), using octadecylsilane combined with silica gel as filler, C18 chromatographic column, using octadecylsilane combined with silica gel as filler, chromatographic column: Elite Kromasil C 18 , 5μm4...

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Abstract

The invention discloses an anti-hemorrhoidal extract and a preparation method thereof. The extract is extracted from bud of Chinese scholartree, flower of Japanese pagoda tree or fruit of citron or trifoliate orange, and contains 55-90% of total flavonoids; wherein, the content ratio of rutin to naringin by percentage is 0.8-4:1. An industrialized process for preparing the extract is preferably achieved by taking medicinal materials such as the bud of Chinese scholartree and the fruit of citron or trifoliate orange as sources according to the process for preparing macroporous resin and water boiling and alcohol precipitation method. The selected process for preparing the macroporous resin has the advantages of being simple and convenient, having good reproductivity, effectively concentrating flavonoid ingredients, getting rid of non-flavonoid ingredients such as triterpenoids, steroids and the like, producing the product with readily controlled quality and high content, extracting at a high efficiency, being usable for industrialized production and the like. The invention also provides a pharmaceutical composition containing the anti-hemorrhoidal total flavonoid extract. The anti-hemorrhoidal total flavonoid extract has good therapeutic effects on diseases such as hemorrhoid, various inflammations and the like.

Description

technical field [0001] The invention relates to a preparation method of an anti-hemorrhoid extract containing rutin and naringin. Background technique [0002] Hemorrhoids are a common and frequently-occurring disease. There has been a saying in China that "nine out of ten people have hemorrhoids" since ancient times. Chinese Patent Application No. 200710124647.5, entitled "An Anti-Hemorrhoid Pharmaceutical Composition", discloses an anti-hemorrhoid composition containing rutin and naringin in a weight percentage of 0.8-4:1, which has a significant anti-hemorrhoid effect. And specifically disclosed two preparation methods, one is directly mixing rutin and naringin; the other is direct water extraction or aqueous ethanol extraction, that is, select the medicinal materials Citrus aurantium and Sophora japonica (or Sophora japonica), add water to decoct, Collect the extract, concentrate the extract, dry, and collect to obtain the Chinese medicine extract; or take the Chinese m...

Claims

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Application Information

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IPC IPC(8): A61K36/752A61P9/14A61K31/7048
Inventor 严启新高媛李勇李姿赵金华冯汉林
Owner SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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