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Method for fermentation production of 2,3-butanediol with Chinese medicinal material rich in starch as raw material

A technology of butanediol and traditional Chinese medicinal materials, which is applied in the fields of fermentation production and microbial fermentation production, which can solve the problems that resources have not been developed and utilized, and achieve the effects of improving high-value utilization, reducing pollution, and being easy to obtain materials

Inactive Publication Date: 2011-10-19
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the total area of ​​pueraria in my country is about 6 million mu, and the annual output is more than 1.5 million tons, of which a small part is used to prepare Chinese medicinal materials and starch processing. It is also reported that Pueraria is used to produce fuel ethanol (Chinese patent: CN101191134; Patent: CN101191134), there are quite a lot of resources that have not been developed and utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Example 1: Carry out shake flask culture with the saccharification liquid of Dioscorea baicalensis as raw material

[0018] (1) Strain: Klebsiella pneumoniae, preserved by China Center for Industrial Microorganism Culture Collection (CICC), with the preservation number CICC No. 10011.

[0019] (2) Liquefaction and saccharification of Dioscorea scutellariae: Grind the dried medicinal materials of Dioscorea scutellaria scutellariae, prepare slurry according to the ratio of material to water 1:4, and pregelatinize at 80-90°C for 30 minutes. Add 12 U / g of high-temperature resistant α-amylase, control the temperature at 90-95°C, liquefy for 30 minutes, then cool down, adjust the pH value to 4.2 with 8% (w / w) hydrochloric acid, add 120 U / g of glucoamylase, The temperature was controlled at 58-60° C., saccharified for 12 hours, and the saccharified liquid was centrifuged at 8000 rpm to obtain yam saccharified liquid with a reducing sugar concentration of 134.4 g / L.

[0020] (...

example 2

[0022] Example 2: Shake flask culture with yam saccharification liquid as raw material

[0023] (1) bacterial classification: with example 1

[0024](2) Liquefaction and saccharification of yam: wash fresh yam, dry and pulverize. According to the ratio of material to water 1:5, pregelatinize at 80-90°C for 30 minutes. Add 12 U / g of high-temperature resistant α-amylase, control the temperature at 90-95°C, liquefy for 30 minutes, then cool down, adjust the pH value to 4.2 with 8% (w / w) hydrochloric acid, add 120 U / g of glucoamylase, The temperature was controlled at 58-60° C., and the saccharification was carried out for 12 hours to obtain a yam saccharification solution with a reducing sugar concentration of 320 g / L.

[0025] (3) Medium: Separately sterilize the yam saccharification solution at 115°C for 15 minutes, and mix it with the inorganic salt nutrients in the sterilized medium after cooling; the inorganic salt nutrients in the medium include: (NH 4 ) 2 HPO 4 6.0g / L...

example 3

[0027] Example 3: Batch fermentation with the saccharification liquid of Dioscorea baicalensis as raw material

[0028] (1) bacterial classification: with example 1

[0029] (2) liquefaction and saccharification of Dioscorea scutellariae: same as example 1

[0030] (3) Medium: Separately sterilize the yam saccharification solution at 115°C for 15 minutes, and mix it with the inorganic salt nutrients in the sterilized fermentation medium after cooling; the inorganic salt nutrients in the fermentation medium include: KH 2 PO 4 1.36g / L; (NH 4 ) 2 SO 4 6.61g / L; MgCl 2 ·6H 2 O0.26g / L; yeast powder 1g / L; citric acid 0.42g / L.

[0031] (4) Culture method and result: use 5L fermenter for fermentation culture, liquid volume is 1.5L, inoculum size is 5% (v / v), fermentation temperature is 37 ℃, rotating speed is 300rpm, air flow rate is 0.1vvm, uses 5mol / L NaOH controls the pH value to 6.0. Batch fermentation was adopted for 19.5 hours of fermentation, the final concentration of ...

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Abstract

The invention discloses a method for producing 2,3-butanediol by using Chinese herbal medicines which are rich in starch as raw materials for fermentation, belonging to the bio-chemical technology field. The method is characterized in that the Chinese herbal medicines which are rich in the starch are taken as the raw materials, saccharified liquid is obtained by liquefaction and saccharification,the saccharified liquid is taken as a carbon source and mixed with inorganic salt nutrition ingredients after sterilization, a strain for producing the 2,3-butanediol is inoculated for carrying out the flask-shaking fermentation, the batch fermentation or the fed-batch fermentation through the supplement of solid glucose, thereby obtaining the 2,3-butanediol with higher concentration. The method has the effects and the advantages that, the raw materials are easy to take, the problems of competing grain with people, competing land with grain, and the like do not exist, the method can effectively reduce the production cost of the 2, 3-butanediol by using the biotransformation method and simultaneously obtain the 2, 3-butanediol with the higher concentration, thereby having broad prospect for high-value utilization of the Chinese herbal medicines.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for fermenting and producing 2,3-butanediol, in particular to a method for producing 2,3-butanediol through microbial fermentation by taking Chinese medicinal materials rich in starch as raw materials. Background technique [0002] 2,3-Butanediol (English name: 2,3-Butanediol, hereinafter referred to as 2,3-BD), as an extremely important chemical raw material, is widely used in chemical, food, aerospace and other fields. Its calorific value is close to that of ethanol, and it is a valuable liquid fuel. It is dehydrated into methyl ethyl ketone, which can be used as a solvent for resins and paints or as a fuel additive; dehydrated into butadiene, which can be used in synthetic rubber; dehydrogenated into acetoin and diacetyl compounds, which can be used to make high-value spices and food additives; It can also replace 1,4-butanediol for the synthesis of polyes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/01C12R1/12C12R1/22
Inventor 修志龙刘国兴董悦生戴建英
Owner DALIAN UNIV OF TECH
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