Mesoporous carbon immobilized enzyme biological sensing material doped with nitrogen and preparing method thereof

A nitrogen-doped mesoporous carbon, immobilized enzyme technology, applied in the fields of biocatalysis and biosensing, can solve the problem of poor biocompatibility of mesoporous carbon materials, unsuitable for large biomolecular loads, and small pore size of mesoporous carbons. and other problems, to achieve the effect of environmental friendliness, strong practicability and high thermal stability
CN101435792AInactive Publication Date: 2009-05-20SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
Publication Date
2009-05-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a nitrogen-doped mesoporous carbon immobilized enzyme biosensing material and a preparation method thereof, which belongs to the field of biocatalysis and biosensing. Mesoporous carbon is prepared by taking mesoporous silica as a template and ethylenediamine, acrylonitrile or tripolycyanamide as a carbon-nitrogen source, and then nitric acid is used to modify the surface of the mesoporous carbon to obtain hydrophilically modified nitrogen-doped mesoporous carbon. The material has high specific surface area, large pore volume, large aperture and good biocompatibility, and also has good water and thermal stability and electric conductivity at the same time. The activity of enzyme molecules mobilized by taking the material as a carrier is well maintained. The biosensing based on nitrogen-doped mesoporous carbon immobilized enzyme has quick response time, wide linear range, high sensitivity, and low detection limit. In addition, a preparation process for the immobilized enzyme biosensing material is simple and controllable, and has the advantages of mild conditions, easily-obtained raw materials, and conventional equipment.
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Description

technical field

[0001] The invention relates to a nitrogen-doped mesoporous carbon-immobilized enzyme biosensing material and a preparation method thereof. It belongs to the field of biocatalysis and biosensing. Background technique

[0002] Biosensors are sensors composed of immobilized cells, enzymes, antibodies or other biologically active substances and signal converters. It is a new type of information technology developed by the interpenetration of biomedicine, electronics and engineering in recent years. It has the advantages of strong specificity, high sensitivity, fast response, low cost and long service life. , Biosensors have a wide range of application values ​​in medicine, medicine, agriculture, animal husbandry and other disciplines, food, fermentation, pharmaceutical and other industrial sectors, and environmental monitoring.

[0003] The selection of appropriate carrier materials to immobilize biomolecules is the key technology to determine the characterist...

Claims

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