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Method for hierarchical barrier separation fine purification medicinal dextran based on repeated washing treatment

A technology for dextran and water washing treatment, which is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of difficulty in concurrently preparing dextran products, error in molecular weight distribution range, and difficulty in industrial application, etc. The effect of reducing the phenomenon of concentration polarization, good uniformity and reducing waste liquid discharge

Inactive Publication Date: 2009-09-30
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the membrane technology used in the production of dextran mainly includes graded membrane separation technology, that is, filter membranes with a certain molecular weight cut-off (the characterization of the membrane pore size) are selected from large to small to filter the fermentation broth step by step, and then undergo decolorization, desalination and The finished product is obtained by drying treatment. Due to the interference of various macromolecular substances such as protein, enzyme preparation or bacteria in the fermentation liquid, the membrane performance is obviously restricted, and it is difficult to prepare multiple dextran products with different molecular weight distributions in parallel at the same time. Therefore, it is necessary to choose A large-scale membrane device with a large effective membrane area leads to a large investment and is difficult for industrial application
[0004] The study also found that dextran molecules spread out linearly in the solution, and larger dextran molecules can penetrate the filter membrane with a smaller molecular weight cut-off. Therefore, there is a large error in simply using the molecular weight cut-off range of the filter membrane to calibrate the molecular weight distribution range of the product.

Method used

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  • Method for hierarchical barrier separation fine purification medicinal dextran based on repeated washing treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of medicinal dextran 40 and dextran 70 by using crude dextran 70

[0033] The preparation of the first step dextran raw material solution:

[0034] Use the crude product of dextran 70 to prepare a raw material solution with a concentration of 3% (W / V), and dissolve the water at a temperature of 50°C; the mechanical stirring speed is 50r / min, and stir for 1.0h; when the temperature of the raw material liquid drops to 40°C, use a 400 mesh screen Filter to remove granular insoluble matter, pump into the raw material tank of the membrane system, and set aside.

[0035] Step 2: Fractional membrane separation based on repeated water washing

[0036] The total volume of the internal feed-liquid pipeline of the first three-stage membrane separation system composed of microfiltration and ultrafiltration membrane devices is 15L, and the total volume of the internal feed-liquid pipeline of the last-stage reverse osmosis device is 40L.

[0037] Microfiltrati...

Embodiment 2

[0043] Example 2 Preparation of medicinal dextran 30 and dextran 40 by using crude dextran 40

[0044] The preparation of the first step dextran raw material solution:

[0045] Use the crude product of dextran 40 to prepare a raw material solution with a concentration of 6% (W / V), and dissolve the water at a temperature of 70°C; the mechanical stirring speed is 100r / min, and stir for 0.5h; when the temperature of the raw material solution drops to 40°C, use a 300-mesh Filtrate to remove granular insoluble matter; pump into the raw material barrel of the membrane system for standby.

[0046] Step 2: Fractional membrane separation based on repeated water washing

[0047] The total volume of the internal feed-liquid pipelines of the first three-stage membrane separation system and the last-stage reverse osmosis device is the same as in Example 1.

[0048] Microfiltration membrane separation is the first stage of membrane separation. Batch feeding is used for continuous filtrati...

Embodiment 3

[0053] Example 3 Parallel preparation of medicinal dextran 20, 40, 70 by using the crude product of dextran 40

[0054] The preparation of the first step dextran raw material solution:

[0055] Use crude dextran 40 to prepare a raw material solution with a concentration of 10% (W / V), dissolve in water at a temperature of 80°C; mechanically stir at a speed of 200r / min, and stir for 2.0h; when the temperature of the raw material solution drops to 40°C, filter it with four layers of gauze To remove granular insoluble matter, pump it into the raw material barrel of the membrane system for standby.

[0056] Step 2: Fractional membrane separation based on repeated water washing

[0057] The total volume of the internal feed-liquid pipelines of the first three-stage membrane separation system and the last-stage reverse osmosis device is the same as in Example 1.

[0058] The feeding volume of microfiltration membrane separation is 300L each time. When the volume of the retentate is...

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Abstract

A method for hierarchical barrier separation fine purification medicinal dextran based on repeated washing treatment, which confects dextran raw charge liquor using dextran crude product, reclaims the process water after filtration with last stage reverse osmosis membrane by using the hierarchical film in the use of water expulsion and cutoff liquor repeated washing, and then the medicinal dextran product is done after concentrated and dried. The invention employs fine filtration, hyperfiltration and reverse osmosis, repeatedly washes trapped fluid of high concentration, sufficiently alleviates the concentrated difference polarization phenomena of filter membrance, thoroughly exerts the choosing effect of filter membrance, detracts dextran molecule more efficaciously, makes the molecular weight distribution of the product more centralization and better uniformity, achieves at paralleling preparing of the family series dextran preparation, improves productivity effect, reduces cost off production, and diminishes discharge of devil liquor.

Description

technical field [0001] The invention belongs to a method for separating and refining dextran used in pharmaceuticals based on repeated water washing treatment by graded membranes. Background technique [0002] Dextran is a high-molecular glucose polymer obtained by dehydrating and polymerizing sucrose through the fermentation of Leuconostoc enterococcus. It is a typical functional microbial polysaccharide and has become a carbohydrate drug widely used in clinical practice. my country included dextran in the national pharmacopoeia in 2000, mainly including dextran 20 (average molecular weight about 20,000), dextran 40 (average molecular weight about 40,000) and dextran 70 (average molecular weight about 70,000). Since the dextran production process adopts the traditional process of acid hydrolysis and ethanol graded precipitation, a large amount of ethanol is consumed, the solubility of the product is affected, and the problem that the molecular weight distribution range of t...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/721
Inventor 陈山李凯邹青松刘丽娅陈玮王军韦艳君李素霞
Owner GUANGXI UNIV