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Method for extracting low-acyl gellan gum suitable for tissue culture medium

A low-acyl gellan gum and tissue culture medium technology, applied in the field of microbial extraction, can solve problems such as milky white flocs that are difficult to dissolve, and achieve good dispersion, high gel strength, and improved product quality.

Active Publication Date: 2009-12-02
ZHEJIANG DSM ZHONGKEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 2. Generally, food-grade gellan gum needs to strictly abide by the order of dissolving the colloid first and then adding metal ions in the specific use process. If the gellan gum powder and metal salt are mixed and then dissolved, the dissolution will be very difficult and a large amount of milky white flocs will be produced. shape

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] A. Deacylation of fermentation broth

[0077] In the flocculation tank, 10m 3 The temperature of the gellan gum fermentation broth was raised to 90°C, and ascorbic acid was added under stirring to a concentration of 150ppm; then 10% KOH was added to adjust the pH to 10.0, and slowly stirred at 90°C for 10 minutes; then 10% hydrochloric acid was added , The pH is adjusted back to 7.0, and the resulting solution enters the next step.

[0078] B. Enzyme treatment of fermentation broth

[0079] Add 15kg of cellulase at 45°C and stir slowly for 5.5 hours under stirring; then add 2kg of lysozyme at 35°C and stir slowly for 3 hours; then add 4kg of neutral protease at 33°C and stir slowly for 2.5 hours.

[0080] C. Flocculation of fermentation broth

[0081] Slowly add 30kg of magnesium chloride to the above material liquid, stirring for 20min, then add 20kg of sodium hydroxide, stirring for 10min, the material is pumped into the box plate and frame filter press for filtration, an...

Embodiment 2

[0093] A. Deacylation of fermentation broth

[0094] In the flocculation tank, 10m 3 The gellan gum fermentation broth is heated to 90°C, add iso-VC sodium to a concentration of 200ppm under stirring; then add 10% NaOH to adjust the pH to 10.0, slowly stir at 90°C for 10 minutes; then add 10% concentration The pH of the acetic acid is adjusted back to 7.0, and the resulting solution enters the next step.

[0095] B. Enzyme treatment of fermentation broth

[0096] Add 15kg of cellulase at 43°C and stir slowly for 5 hours while stirring; then add 2kg of lysozyme at 33°C and stir slowly for 3 hours; then add 4kg of alkaline protease at 35°C and stir slowly for 3 hours.

[0097] C. Flocculation of fermentation broth

[0098] Slowly add 35kg of calcium chloride to the above-mentioned material liquid, keep stirring for 20min, then add 20kg of potassium hydroxide, keep stirring for 10min, pump the material into the box plate and frame filter press for filtration, and the filtrate enters ...

Embodiment 3

[0110] A. Deacylation of fermentation broth

[0111] In the flocculation tank, 10m 3 The temperature of the gellan gum fermentation broth was raised to 90°C, potassium metabisulfite was added under stirring to a concentration of 250ppm; then 10% KOH was added to adjust the pH to 10.0, and the temperature was slowly stirred at 90°C for 10 minutes; then 10% was added With the concentration of citric acid, the pH is adjusted back to 7.0, and the resulting solution enters the next step.

[0112] B. Enzyme treatment of fermentation broth

[0113] Add 15kg of cellulase at 43°C and stir slowly for 5 hours while stirring; then add 2kg of lysozyme at 33°C and stir slowly for 3 hours; then add 4kg of alkaline protease at 35°C and stir slowly for 3 hours.

[0114] C. Flocculation of fermentation broth

[0115] Slowly add 35kg of zinc chloride to the above liquid, stirring for 20min, then add 20kg of sodium carbonate, stirring for 10min, pump the material into the box-type filter press for fi...

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Abstract

The invention relates to a method for extracting low-acyl gellan gum suitable for a tissue culture medium, which comprises the following steps of: acyl removal treatment and enzyme treatment of a gellan gum fermenting fluid, flocculation of the gellan gum in a low-acyl state by bivalent or multivalent metal cation, clarification treatment of a gellan gum solution, dehydration treatment of the gellan gum solution, decoloring and ion exchange treatment, addition of a chelator / acid system to chelate bivalent cation possibly added during using the gellan gum and relative stability keeping of pH value, drying, and pulverization. The invention also provides the low-acyl gellan gum prepared by the method; the low-acyl gellan gum has the characteristics of good product appearance, good transmittance and high gelatinization strength which include the following properties: the chrominance is more than 83 percent; the transmittance is over 90 percent; and simultaneously, the gelatinization strength is between 400 and 650 g / cm.

Description

Technical field [0001] The invention relates to the field of microorganism extraction, in particular to a post-extraction method of low acyl gellan gum suitable for tissue culture medium. Background technique [0002] Gellan gum is a hydrophilic colloid produced by fermentation of Sphingomonas paucimobilis, a bacteria isolated from nature through extensive screening. It has a variety of useful properties. [0003] The basic molecular structure of gellan gum is a main chain consisting of repeating tetrasaccharide units. The monosaccharides involved in the formation are glucose, rhamnose and glucuronic acid. In the original form of gellan gum, which is high acyl gellan gum, two kinds of substituents-acetyl substituent and glyceryl substituent are present. Both groups are located on the same glucose residue, each repeating unit has a glyceryl substituent, and every two repeating units has an acetyl substituent. In low acyl gellan gum, acyl groups are completely removed. Acyl groups ...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02
Inventor 吴炳华朱正学王雪刚王佳良包圣强沈孝琴
Owner ZHEJIANG DSM ZHONGKEN BIOTECH
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