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Method for extracting rapeseed protein by reverse micelle

A rapeseed protein and reverse micelle technology, which is applied to the preparation methods of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of extracting rapeseed protein without reverse micelles, etc., and achieves low production cost and protein. The effect of high extraction rate and mild operating conditions

Inactive Publication Date: 2010-01-06
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no research on extracting rapeseed protein from reverse micelles

Method used

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  • Method for extracting rapeseed protein by reverse micelle
  • Method for extracting rapeseed protein by reverse micelle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of rapeseed meal powder:

[0024] The high-quality double-low rapeseed produced in 2008 is selected, hulled on the hulling machine, and then subjected to winnowing. The dehulled rapeseed is crushed on a hammer cyclone mill, and then passed through a 100 mesh sieve. Use petroleum ether (30-60°C boiling range) to deoil the crushed rapeseed on a Soxhlet extractor at 50°C. After treatment, place it in a ventilated place for desolvation to make the petroleum ether volatilize completely. Then ventilate and dry, and the obtained rapeseed meal powder is required for the test, and its protein (N×6.25 dry basis) content is determined to be 50.1%.

[0025] (2) Configuration of reverse micelle solution

[0026] KCl buffer solution preparation: Weigh 110.8g disodium hydrogen phosphate dodecahydrate, 9.07g potassium dihydrogen phosphate, make 1L solution, take 190mL and 10mL respectively and mix well, then add 0.596g potassium chloride to the above mixture To completely d...

Embodiment 2

[0032] (1) Preparation of rapeseed meal powder:

[0033] The high-quality double-low rapeseed produced in 2008 is selected, hulled on the hulling machine, and then subjected to winnowing. The dehulled rapeseed is crushed on a hammer cyclone mill, and then passed through an 80 mesh sieve. Use petroleum ether (30-60°C boiling range) to deoil the crushed rapeseed on a Soxhlet extractor at 50°C. After treatment, place it in a ventilated place for desolvation to make the petroleum ether volatilize completely. Then ventilate and dry, and the obtained rapeseed meal powder is required for the test, and the protein content (N×6.25, dry basis) is measured to be 45.6%.

[0034] (2) Preparation of reverse micelle solution

[0035] KCl buffer solution preparation: prepare 0.2mol / L disodium hydrogen phosphate solution and 0.1mol / L citric acid solution 1L each, take 10.30mL and 9.70mL respectively and mix well. Then add 0.1193g potassium chloride to the above mixed solution to completely dissolv...

Embodiment 3

[0042] (1) Preparation of rapeseed meal powder:

[0043] The high-quality common rapeseed produced in 2008 was selected, hulled on the hulling machine, and then air-selected. The dehulled rapeseed is crushed on a hammer cyclone mill, and then passed through an 80 mesh sieve. Use petroleum ether (30-60°C boiling range) to deoil the crushed rapeseed on a Soxhlet extractor at 50°C. After treatment, place it in a ventilated place for desolvation to make the petroleum ether volatilize completely. Then ventilate and dry, and the obtained rapeseed meal powder is required for the test, and its protein (N×6.25, dry basis) content is measured to be 43.21%.

[0044] (2) Preparation of reverse micelle solution:

[0045] KCl buffer solution preparation: prepare 50mL 0.05mol / L disodium hydrogen phosphate solution and 4.1mL 0.1mol / L sodium hydroxide, mix well, add water to dilute to 100mL, and add 1.118g potassium chloride to the above mixture , Make it completely dissolved. A KCl buffer soluti...

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Abstract

The invention relates to a method for extracting rapeseed protein by reverse micelle, comprising the following steps: adding rapeseed powders into a reverse micelle solution, extracting, and then obtaining a rapeseed protein solution by solid-liquid separation. As a preferable scheme, the reverse micelle solution is prepared by mixing Sodium di-n-octyl sulfosuccinat, isooctane and a buffer solution containing K<+>. The addition of the rapeseed powders is 0.001-0.20 g / mL. As an improvement of the invention, the extraction is carried out under the action of an ultrasonic wave. The frequency of a preferable ultrasonic wave is 20 KHz, and the power is 100 KW. The invention has simple process, low production cost, mild operating conditions, high product purity as well as high protein extraction efficiency, and does not need to add a great amount of acid and alkali.

Description

Technical field [0001] The invention relates to a method for extracting rapeseed protein from reverse micelles. Background technique [0002] Rape is an oil crop produced in a large area in my country, and its output ranks first in the world. The amount of by-products produced in the processing of rapeseed oil is huge. The output of rapeseed meal alone exceeds 10 million tons per year. Rapeseed meal contains 35% to 42% of crude protein, and the content of lysine, cystine and methionine in rapeseed protein is higher, and the amino acid balance is better than that of soybean protein. It is a source of plant protein. [0003] At present, using rapeseed or rapeseed meal as raw materials, the methods for producing rapeseed protein mainly include aqueous phase method, aqueous phase enzymatic hydrolysis, organic solvent extraction, ultrafiltration, percolation and other methods. In industrial production, the aqueous phase method often has a low protein extraction rate, and waste water i...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/14
Inventor 鞠兴荣王立峰朱均旺何荣袁建
Owner NANJING UNIV OF FINANCE & ECONOMICS
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