Method for extracting rapeseed protein by reverse micelle
A rapeseed protein and reverse micelle technology, which is applied to the preparation methods of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of extracting rapeseed protein without reverse micelles, etc., and achieves low production cost and protein. The effect of high extraction rate and mild operating conditions
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[0022] Example 1:
[0023] (1) Preparation of rapeseed meal powder:
[0024] The high-quality double-low rapeseed produced in 2008 is selected, hulled on the hulling machine, and then subjected to winnowing. The dehulled rapeseed is crushed on a hammer cyclone mill, and then passed through a 100 mesh sieve. Use petroleum ether (30-60°C boiling range) to deoil the crushed rapeseed on a Soxhlet extractor at 50°C. After treatment, place it in a ventilated place for desolvation to make the petroleum ether volatilize completely. Then ventilate and dry, and the obtained rapeseed meal powder is required for the test, and its protein (N×6.25 dry basis) content is determined to be 50.1%.
[0025] (2) Configuration of reverse micelle solution
[0026] KCl buffer solution preparation: Weigh 110.8g disodium hydrogen phosphate dodecahydrate, 9.07g potassium dihydrogen phosphate, make 1L solution, take 190mL and 10mL respectively and mix well, then add 0.596g potassium chloride to the above mixtu...
Example Embodiment
[0031] Example 2:
[0032] (1) Preparation of rapeseed meal powder:
[0033] The high-quality double-low rapeseed produced in 2008 is selected, hulled on the hulling machine, and then subjected to winnowing. The dehulled rapeseed is crushed on a hammer cyclone mill, and then passed through an 80 mesh sieve. Use petroleum ether (30-60°C boiling range) to deoil the crushed rapeseed on a Soxhlet extractor at 50°C. After treatment, place it in a ventilated place for desolvation to make the petroleum ether volatilize completely. Then ventilate and dry, and the obtained rapeseed meal powder is required for the test, and the protein content (N×6.25, dry basis) is measured to be 45.6%.
[0034] (2) Preparation of reverse micelle solution
[0035] KCl buffer solution preparation: prepare 0.2mol / L disodium hydrogen phosphate solution and 0.1mol / L citric acid solution 1L each, take 10.30mL and 9.70mL respectively and mix well. Then add 0.1193g potassium chloride to the above mixed solution to...
Example Embodiment
[0041] Example 3:
[0042] (1) Preparation of rapeseed meal powder:
[0043] The high-quality common rapeseed produced in 2008 was selected, hulled on the hulling machine, and then air-selected. The dehulled rapeseed is crushed on a hammer cyclone mill, and then passed through an 80 mesh sieve. Use petroleum ether (30-60°C boiling range) to deoil the crushed rapeseed on a Soxhlet extractor at 50°C. After treatment, place it in a ventilated place for desolvation to make the petroleum ether volatilize completely. Then ventilate and dry, and the obtained rapeseed meal powder is required for the test, and its protein (N×6.25, dry basis) content is measured to be 43.21%.
[0044] (2) Preparation of reverse micelle solution:
[0045] KCl buffer solution preparation: prepare 50mL 0.05mol / L disodium hydrogen phosphate solution and 4.1mL 0.1mol / L sodium hydroxide, mix well, add water to dilute to 100mL, and add 1.118g potassium chloride to the above mixture , Make it completely dissolved. ...
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