Microorganism for hydrolyzing 7-xylose group and 13-side chain of taxane

A technology of taxanes and taxanes, which is applied in the field of enzymes and taxanes, can solve the problems of unstable production capacity of yew cells, complicated preparation process of taxanes, and inability to industrialize production. The effect of large-scale industrial production, strong biological activity, and simplified preparation process
CN101684451AInactive Publication Date: 2010-03-31INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
Publication Date
2010-03-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to enterobacter cloacae which are found in soil and have the collection number of CGMCC No.2487 or resting cell with equivalent function, variant and mutant as well as enzyme produced thereby. The invention also provides a preparation method of taxane, namely comprising the following steps: the enterobacter cloacae are used for hydrolyzing 7-xylose group in 7-xylose taxane molecule in wheat bran culture medium, and 13-side chain in taxane molecule with 13-side chain can be hydrolyzed in peptone beef extract culture medium; and the produced taxane can be used for preparing antitumor drugs, such as intermediates of taxane drugs, docetaxel drugs or taxane drugs.
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Description

Technical field:

[0001] The present invention relates to a kind of preparation method of Enterobacter cloacae (Enterobactercloacae) or its functionally equivalent quiescent cells, variants and mutants whose strain preservation number is CGMCC No.2487 and the enzyme and taxane produced thereof, specifically , involving a 7-xylose group that can hydrolyze the 7-xylose taxane in the wheat bran medium, and can also hydrolyze the 13- Enterobacter cloacae with side chains, and a method for preparing taxanes using Enterobacter cloacae. Background technique:

[0002] Paclitaxel is a diterpene alkaloid with unique anticancer activity isolated from the bark of Taxus brevifolia by Wani et al. in 1971. Its chemical structural formula is:

[0003]

[0004] Since paclitaxel was approved by the FDA in 1992 for the treatment of ovarian cancer and metastatic breast cancer, it has been approved for marketing in more than 40 countries. In addition, it has been found that they also have g...

Claims

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