Method for preparing galactose chitosan/polyester polymer composite stent

A technology of chitosan and composite scaffolds, which is applied in the field of preparation of scaffolds for liver repair, can solve the problems of difficulty in meeting the application requirements of bioartificial livers, single scaffold components, poor mechanical properties, etc., and achieve good biocompatibility and The effect of biological activity, wide range of material sources and high production efficiency

Inactive Publication Date: 2010-08-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of scaffold has a single component, and has defects such as poor mechanical properties, difficulty in processing, and rapid degradation, and it is difficult to meet the application requirements of bioartificial liver and other treatment methods.

Method used

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  • Method for preparing galactose chitosan/polyester polymer composite stent
  • Method for preparing galactose chitosan/polyester polymer composite stent
  • Method for preparing galactose chitosan/polyester polymer composite stent

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] (1) Dissolve galactosylated chitosan in distilled water, prepare a galactosylated chitosan solution with a concentration of 0.01g / mL, leave it to defoam; dissolve polycaprolactone in dioxane, Prepare a polymer solution with a concentration of 0.1g / ml;

[0027] (2) Pour the 280-450μm gelatin particles sieved with a standard test sieve into the mold, and use a syringe to inject the 85% ethanol solution into the mold to immerse the particles; place the mold in a 70°C oven 5 minutes, make the gelatin particles bond into a template, take out the mold, leave it for a few days until the ethanol is volatilized, freeze-dry; take out the gelatin template, immerse it in the polycaprolactone solution, repeatedly use the method of vacuuming and then returning to normal pressure The polycaprolactone solution is introduced into the template, freeze-dried, and then soaked in 37°C hot water until the gelatin is completely dissolved to obtain the polycaprolactone porous scaffold; figure 1 A...

example 2

[0032] The steps are the same as those in Example 1, but the galactosylated chitosan / polycaprolactone composite scaffold was obtained by reacting in the galactosylated chitosan aqueous solution under stirring at 37°C for 16 hours.

example 3

[0034] The steps are the same as those in Example 1, but the dioxane solution of polylactic acid-glycolic acid copolymer is used, and the solution is treated in 0.5mol / L sodium hydroxide solution for 60 minutes, and then in carbodiimide salt Activate for 12 hours in a 1:1 mixed solution of acid salt and N-hydroxysuccinimide by molar ratio, and finally react for 4 hours in an aqueous solution of galactosyl chitosan at 37°C under stirring to obtain galactosyl chitosan. Sugar / polylactic acid-glycolic acid copolymer composite scaffold.

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Abstract

The invention discloses a method for preparing a galactose chitosan / polyester polymer composite stent, which comprises the following steps: dissolving galactose chitosan in distilled water to prepare solution; preparing a porous polyester polymer stent by using gelatin particles as a porogenic agent; treating the polyester polymer stent in alkaline solution and then activating the polyester polymer stent with mixed solution of carbodiimide hydrochloride and N-hydroxysuccinimide; and immersing the activated polyester polymer stent into the solution of the galactose chitosan and performing freeze drying after reaction to produce the galactose chitosan / polyester polymer composite stent with biological activity. The galactose chitosan / polyester polymer composite stent prepared by the method has high mechanical strength, can provide a growth space for liver cells, has high biocompatibility and biological activity, and can promote the growth of the liver cells and the maintenance of functions. The galactose chitosan / polyester polymer composite stent has the advantages of simple preparation method, controllable size and shape of materials, high production efficiency, and good applicationprospect in the aspects of bioartificial livers and liver tissue repair.

Description

Technical field [0001] The invention relates to a method for preparing a scaffold for liver repair, in particular to a method for preparing a galactosylated chitosan / polyester polymer composite scaffold. Background technique [0002] The liver is one of the important organs of the human body, which is responsible for many functions such as metabolism, detoxification and the release of carbohydrate proteins. However, humans are often plagued by various liver diseases. The number of patients suffering from various liver diseases such as viral hepatitis, fatty liver disease, and alcoholic liver disease is increasing year by year. Acute liver failure caused by various chronic liver diseases has a very high fatality rate. Although liver transplantation is currently the most effective treatment for liver failure, it faces problems such as shortage of liver donors, large surgical wounds, and high cost. With the research and development of tissue engineering and regenerative medicine,...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/18
Inventor 高长有邱媛章继川劳丽红
Owner ZHEJIANG UNIV
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