L-arginine producing strain corynebacterium glutamicum and preparation method and application

Abstract

The invention discloses L-arginine producing strain corynebacterium glutamicum ZD2009, CCTCC NO:M209260 and a preparation method and application. The preparation method comprises the following steps of: A, performing ultraviolet mutagenesis, namely culturing the corynebacterium glutamicum, centrifuging, removing supernatant, washing, and scattering the strain by using glass beads to obtain cell suspension; B, performing 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis, namely culturing the corynebacterium glutamicum, centrifuging, removing the supernatant, washing, scattering the strain by using the glass beads, washing with phosphate buffer after washing, adding 1-methyl-3-nitro-1-nitrosoguanidine for mutagenesis, washing with the phosphate buffer to remove the 1-methyl-3-nitro-1-nitrosoguanidine and stopping the mutagenesis; C, determining shake flask fermentation conditions; D, determining a fermentation medium and inoculation amount; E, determining pH value; and F, determining liquid loading amount. The acid producing amount of the L-arginine producing strain corynebacterium glutamicum reaches 44g / L; and the strain produces the acid stably, has high genetic stability, reduces production cost and improves efficiency.

Description

Technical field [0001] The present invention relates to the field of biotechnology, more specifically to a L-arginine-producing bacterium Corynebacterium glutamicum, and a preparation method of L-arginine-producing bacterium Corynebacterium glutamicum, and also to L-arginine Use of the producing strain Corynebacterium glutamicum in animal feed. Background technique [0002] L-arginine is the final product of the arginine biosynthetic pathway, a semi-essential basic amino acid for humans and animals, and an essential amino acid for young animals. L-arginine has a wide range of uses. In the pharmaceutical industry, L-arginine is an important intermediate metabolite in the urea cycle of organisms. It is also one of the main components of compound amino acid infusion, and it is also used to formulate nutrition or special treatment products. It is widely used as an antidote for ammonia-toxic hepatic coma and a promoter of liver function. It has a significant effect on viral hepatitis...

AI Technical Summary

Problems solved by technology

At present, the domestic research on the production of L-arginine by fermentation is limited to the laboratory level, which is far behind the international technology. Therefore, it is of great significance to breed high-yielding strains of L-arginine and optimize its fermentation process.

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