Ocean source Bacillus barbaricus SCSIO 02429 and method for preparing squid small peptide by using same

A technology for squid and squid viscera is applied in the field of preparing squid small peptides, which can solve the problems of excessive hydrolysis, difficult absorption and utilization of crude protein, low hydrolysis rate of crude protein, etc. The effect of reducing seedling mortality

CN101974460AActive Publication Date: 2011-02-16SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI

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Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date: 2011-02-16

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Abstract

The invention relates to ocean source Bacillus barbaricus SCSIO 02429 CCTCC No: M2010213. The invention also relates to a method for preparing squid small peptide, which is characterized by comprising the following steps of: adding the Bacillus barbaricus SCSIO 02429 into an inducing enzyme-production fermentation medium to ferment to obtain a crude enzyme solution for hydrolysis; then, after crashing squid abdominal organs into pulp, and adding the crude enzyme solution to carry out zymolysis to obtain a crude protein zymolyte with a destroyed glycosyl side chain; adding bromelin to carry out zymolysis; standing the obtained zymolyte; separating a crude adipose layer and a water layer; and drying after removing the crude adipose layer to obtain the squid small peptide. In the invention, the squid small peptide yield can reach 40-46 percent, and the amino acid yield can reach 16-22 percent. The squid small peptide has the actions of reducing the mortality rate of young seaculture animal and increasing the percentage of liveweight growth, can replace the traditional protein sources of fish meal, and the like in feed and is used for functional protein sources, additives, and the like of seaculture compound feed.

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Description

technical field

[0001] The present invention relates to a bacillus, in particular to a marine bacillus barbaricus SCSIO 02429 strain, and also relates to a method for preparing squid small peptides by using the strain. Background technique

[0002] With the rapid development of my country's marine fishing industry, the annual output of squid has reached about 300,000 tons, and it has become one of the important raw materials for aquatic products processing in my country. During the processing of squid, about 15% of visceral waste is produced. These wastes are rich in protein, but they are extremely perishable and difficult to store. The usual treatment method is to bury the waste after the squid oil is extracted. Recently, there have also been reports that the squid viscera were processed into squid slurries and used directly as fish feed. The rate is not high, and it will also pollute the water environment and increase the chance of disease outbreaks in farmed animals. Th...

Claims

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