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Method for preparing microbial oil by fermenting cassava starch and preparing biomouldsel from microbial oil

A microbial oil and cassava starch technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low reducing sugar yield and cumbersome pretreatment steps, and achieve low price, environmental friendliness, and reduced The effect of production costs

Inactive Publication Date: 2011-02-16
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above inventions use agricultural and sideline product wastes as fermentation raw materials, the preliminary pretreatment steps of raw materials are cumbersome and the yield of available reducing sugars is low, and there are certain defects in large-scale industrial production.

Method used

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  • Method for preparing microbial oil by fermenting cassava starch and preparing biomouldsel from microbial oil
  • Method for preparing microbial oil by fermenting cassava starch and preparing biomouldsel from microbial oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Tapioca starch pretreatment

[0033] Add the cassava starch raw material into tap water and mix it into a starch slurry with the ratio of water and starch raw material mass ratio of 3:1; adjust the pH value of the starch slurry at 5.5, add α-amylase to the starch slurry, and add α-amylase The amount of enzyme is 2U / g dry weight of starch, liquefied at 90°C for 2 hours to obtain starch liquefaction solution; adjust the pH value of starch liquefaction solution to 4.0 and then add glucoamylase, the amount of glucoamylase added is 400U / g dry starch Heavy, saccharified at 60°C for 2 hours to obtain a starch saccharification solution, the concentration of reducing sugar was determined to be 215g / L, and it was used as a carbon source for the fermentation medium for subsequent use;

[0034] 2. Fermentation

[0035] (1) Strain: Trichosporium dermatosa B3

[0036] (2) Culture medium: seed medium: glucose 70g / L, NH 4 NO 3 0.5g / L, yeast extract powder 1g / L, MgSO 4 ·7H 2 O...

Embodiment 2

[0042] 1. cassava starch pretreatment is the same as embodiment 1

[0043] 2. Fermentation

[0044] (1) Strain: Trichosporium dermatosa B3

[0045] (2) culture medium: seed culture medium is the same as embodiment 1

[0046] Fermentation medium: the carbon source is cassava starch saccharification solution, and its dosage is 80g / L based on its reducing sugar content, NH 4 NO 3 0.5g / L, yeast extract powder 3g / L, MgSO 4 ·7H 2 O 0.4g / L, KH 2 PO 4 0.75g / L, CaCl 2 2H 2 O 0.4g / L, pH is 6.0;

[0047] (3) fermentation mode is the same as embodiment 1, and the fermentation culture time is 60h;

[0048] (4) Fermentation result

[0049] After 60 hours of fermentation, its biomass reached 28.2g / L. Petroleum ether was used as an organic solvent combined with a Soxhlet extractor to heat and reflux in a water bath at 80° C. for 6 hours to extract oil from the bacteria, and the oil content reached 36.5%.

Embodiment 3

[0051] 1. Tapioca starch pretreatment

[0052] Add tapioca starch raw material into tap water and mix into starch slurry with the ratio of water and starch raw material mass ratio of 4:1; adjust the pH value of starch slurry at 5.5, add α-amylase to starch slurry, α-amylase adds The amount of enzyme is 6U / g starch dry weight, liquefied at 90°C for 2 hours to obtain starch liquefaction solution; adjust the pH value of starch liquefaction solution to 4.0 and then add glucoamylase, the amount of glucoamylase added is 300U / g starch dry Heavy, saccharified at 60°C for 2 hours to obtain a starch saccharification liquid, the concentration of reducing sugar was determined to be 240g / L, which was used as a carbon source for the fermentation medium for later use;

[0053] 2. Fermentation

[0054] (1) Strain: Trichosporium dermatosa B3

[0055] (2) Culture medium: seed medium: glucose 70g / L, NH 4 NO 3 0.5g / L, yeast extract powder 1g / L, MgSO 4 ·7H 2 O0.4g / L, KH 2 PO 4 0.75g / L, C...

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Abstract

The invention relates to a method for preparing microbial oil by fermenting cassava starch and preparing biodiesel from the microbial oil and belongs to the field of biological energy sources. The method comprises the method for preparing the microbial oil and the method for preparing the biodiesel from the microbial oil and comprises the following steps of: fermenting dermoid Trichosporon B3 serving as an oil producing strain and cassava starch saccharified liquid serving as a carbon source in batches under a certain culturing condition to culture microbes, and drying and extracting to obtain the microbial oil; and performing transesterification reaction on the microbial oil serving as a raw material under the action of a catalyst to obtain the biodiesel, wherein the by-product is glycerine. The method has the advantages that: the cassava starch serving as the carbon source is fermented to prepare the microbial oil, so the cassava starch resource is fully used and the raw material cost for preparing the microbial oil is reduced; a process for transferring the microbial oil into the biodiesel is built; and the method is easy and convenient to operate and control, is environment-friendly, and has good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for producing microbial oil by fermenting tapioca starch and preparing biodiesel. Background technique [0002] Biodiesel is a renewable new energy developed in recent years based on environmental degradation and energy crisis that can replace fossil fuels. It is a renewable clean energy with great development potential. At present, the research on biodiesel preparation at home and abroad mainly focuses on vegetable oil or waste catering oil, but the cost of using vegetable oil as raw material is high, and its cost accounts for 70-85% of the total production cost. With waste catering oil as raw material, there are also raw materials Difficulties in collection and transportation have seriously restricted the industrialization process of biodiesel, so finding a cheap raw material has become the key to the industrialization of biodiesel. [0003] Microbial oi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C10L1/02C12P7/64C11C3/10C12R1/645
CPCY02E50/13Y02E50/10
Inventor 朱笃张志斌颜日明曾庆桂
Owner JIANGXI NORMAL UNIV
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