Brucella diagnostic kit and use method thereof

Abstract

The invention discloses a microbiological detection technology, in particular to a kit for diagnostic detection of Brucella and a use method of the kit. The Brucella detection kit of the invention at least comprises two pairs of specific amplification primers. The invention has the principle that by utilizing the loop-mediated isothermal amplification reaction (LAMP reaction for short), Bst DNA polymerase and two pairs of specific inner and outer primers (inner primers FIP / BIP and outer primers F3 / B3) designed according to a target gene sequence specifically recognize six independent regions on the target sequence, start cyclic chain displacement reaction and start complementary chain synthesis in the target DNA region, so that on the same chain, a complementary sequence cyclically forms a stem-loop DNA mixture with a plurality of loops and a cauliflower structure.

Description

technical field

[0001] The invention relates to a microorganism detection technology, in particular to a kit for diagnosing Brucella and a method for using the kit. Background technique

[0002] Brucellosis is a zoonotic infectious disease caused by Gram-negative, facultative intracellular bacteria of the genus Brucella. Many countries have reported outbreaks of brucellosis in humans and animals. Brucella has a wide host range and can infect more than 60 species of domestic animals, wild animals and marine mammals. After infection, it mainly causes damage to the reproductive system of animals, causes inflammation of the fetal membranes of pregnant female animals, leads to infertility, miscarriage, stillbirth, etc., and seriously endangers the development of animal husbandry and aquaculture. Humans often get infected due to contact with infected animals' milk, meat, fur and animal offal products, causing damage to bones and joints, nervous system, reproductive system, circu...

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