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Multifunctional core-shell structure fluorescent coding magnetic microspheres and preparation method thereof

A fluorescent coding, magnetic microsphere technology, applied in the fields of nanomaterials and biomedicine, can solve the problems of organic molecules occupying surface functional groups and influence, and achieve the effect of good fluorescence performance and magnetic performance

Inactive Publication Date: 2011-07-13
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The synthesis method reported so far mainly includes magnetic nanoparticles as the core, outer silicon layer, and then amination, and then through the reaction of the isothiocyanate of the organic dye molecule with the amino group of the outer layer of the composite material, and the coupling of the organic dye molecule, (Jang J H, Lim H B. Microchem. J. 2010, 94(2): 148-158. Zhang Y, Gong S W Y, Jin L, et al. Chinese Chem. Lett. 2009, 20(8): 969-972.) However, this coupling method will cause organic molecules to occupy the surface functional groups of the composite material, which will affect further reactions. The in situ preparation method we proposed solves this problem very well.

Method used

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  • Multifunctional core-shell structure fluorescent coding magnetic microspheres and preparation method thereof
  • Multifunctional core-shell structure fluorescent coding magnetic microspheres and preparation method thereof
  • Multifunctional core-shell structure fluorescent coding magnetic microspheres and preparation method thereof

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Embodiment 1

[0048] Fluorescence-encoded magnetic microspheres with core-shell structure with a fluorescence ratio (mass ratio) of FITC:RBITC=1:1 were prepared.

[0049] a. Preparation of superparamagnetic iron tetroxide nanoparticles.

[0050] Weigh out ferric chloride hexahydrate (FeCl 3 ·6H 2 O) 1.35g, dissolved in 40mL of ethylene glycol, magnetically stirred for 30min, the ferric chloride was completely dissolved. Then, 3.6g of anhydrous sodium acetate and 1.0g of polyethylene glycol were added in sequence, and the resulting solution was moved to a 50mL Teflon-Lined stainless steel reactor after magnetic stirring for 30min, and reacted at 200°C for 8~16h. After the reaction was completed, the reactor was taken out. Naturally cooled to room temperature, repeatedly washed with ethanol and deionized water for several times, and then dried under vacuum at 60 °C for use.

[0051] b. Preparation of FITC-APTMS and RBITC-APTMS prepolymer.

[0052] Take 1 mL of ethanol and put it in a dry ...

Embodiment 2

[0056] Fluorescence-encoded magnetic microspheres with amino-functionalized core-shell structure with a fluorescence ratio (mass ratio) of FITC:RBITC=4:1 were prepared.

[0057] a. Preparation of superparamagnetic iron tetroxide nanoparticles is the same as step a of Example 1.

[0058] b. Preparation of FITC-APTMS and RBITC-APTMS prepolymer.

[0059] Take 1 mL of ethanol and put it in a dry and clean reaction flask pretreated with 5% hydrofluoric acid, then dissolve 4 mg FITC and 1 mg RBITC in ethanol solution, add 10 μL APTMS under magnetic stirring, dry, seal, avoid light, and stir for 24 h. A mixed prepolymer of FITC-APTMS and RBITC-APTMS was obtained.

[0060] c. Preparation of core-shell structured fluorescently encoded magnetic microspheres.

[0061] In a 50mL round-bottomed flask, add 7.5mL of cyclohexane, 1.77mL of Triton X-100, and 1.8mL of n-hexanol, mechanically vigorously stir for 30min to mix, and then dropwise add 250μL of aqueous solution, continue to stir to...

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Abstract

The invention belongs to the technical field of nano materials and biomedicine, in particular to multifunctional core-shell structure fluorescent coding magnetic microspheres and a preparation method thereof. On the basis of ferroferric oxide nanoparticles synthesized by a hydrothermal process, pre-prepared coupled product of fluoresceins and amino propyl trimethoxysilane and ethyl orthosilicate are subjected to cohydrolysis in ammonia water to form a multifunctional fluorescent magnetic nano composite material with fluorescent and magnetic properties and high biological stability and biological adaptability. By regulating the mixing ratio of fluoresceins, namely fluorescein isothiocyanate (FITC) and rhodamine B isothiocyanate (RBITC), various fluorescent coding magnetic microspheres can be prepared, and the particle size of obtained material can be regulated according to the different ratios of the added ethyl orthosilicate to the ferroferric oxide. In addition, the obtained multifunctional composite nano material is subjected to amino silanization modification, so the biological application range of the novel fluorescent coding magnetic microspheres is further expanded and the novel fluorescent coding magnetic microspheres have bright application prospect in fields of biomedicine technology, medicine development, suspension chip and the like.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biomedicine, and in particular relates to a double-fluorescence-labeled nanomagnetic microsphere and a preparation method thereof. Background technique [0002] At present, magnetic nano-silicon sphere composites have been greatly developed in the field of biotechnology research. Various magnetic microspheres are based on their high separation efficiency, simple sample handling process, harmless operating conditions and easy functional modification. It has been widely used in protein separation, cell labeling, cell detection, cell imaging, etc. In addition, based on the good paramagnetism, easy synthesis and functionalization of the magnetic material ferric oxide, it becomes an important research and development object for separation, enrichment, nuclear magnetic imaging, and immunoassay. The biological adaptability, stability, water-solubility and easy re-modification ability of the Ti...

Claims

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Application Information

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IPC IPC(8): B01J13/02C09K9/00H01F1/11
Inventor 张正勇李会香孔继烈
Owner FUDAN UNIV
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