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Flavonoid glycoside compounds, method for preparing same and application

A technology of flavonoid glycosides and compounds, which is applied in the field of medicine and can solve the problems of no medicinal records, less cat ginseng, etc.

Active Publication Date: 2011-10-12
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only few research reports on the chemical components and pharmacological effects of cat ginseng [Ding Lili, Wang Shunchun, Wang Zhengtao. Research on the chemical components of cat ginseng. Chinese Journal of Traditional Chinese Medicine, 2007, 32(18): 1893-1895; Zhang Yani , Liu Ling, Ling Changquan. Discussion on the inhibitory effect and mechanism of effective parts of cat ginseng on transplanted tumor H22 in mice. Chinese Journal of Traditional Chinese Medicine, 2006, 36(11): 918-920; Lu Yin, Chen Shaoyuan, Wu Ping, et al. Study on the active components of cat ginseng (I)-inorganic element analysis. Journal of Zhejiang University, 2004, 30(2): 185-188; Xin Hailiang, Wu Yingchun, Xu Yanfeng, etc. Comparative study on the root and stem of Actinidia calyx. Journal of the Second Military Medical University, 2008, 29(3): 298-302], there is no medicinal record and research report for the leaves of Actinidia calyx

Method used

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  • Flavonoid glycoside compounds, method for preparing same and application
  • Flavonoid glycoside compounds, method for preparing same and application
  • Flavonoid glycoside compounds, method for preparing same and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: the extraction separation of compound 1, 2

[0065] 15.3Kg of kiwifruit leaves (collected from the mountainous area of ​​Quzhou, Zhejiang Province in September 2006) were extracted with 80% ethanol, 12 times, 10 times, 10 times, 1.5 hours, 1 hour, 1 hour, and the extract was filtered while it was hot , combine the extracts, recover the solvent under reduced pressure until there is no alcohol smell, add distilled water to 15L, 5000rpm, centrifuge for 5 minutes, 5L of the supernatant is absorbed by the MCI-Gel resin column (diameter 10cm×height 120cm), and 50L of water , 10% ethanol, 40% ethanol as eluent elution, 1000ml / part receives, traces the fraction (silica gel GF254 thin-layer plate of detection with TLC, developer: n-butanol-acetic acid-water=4: 1: 5. Upper layer, color development method: visual inspection under 254nm ultraviolet lamp), the fractions containing the target components (concentrated in the 40% ethanol elution part) were combined, reco...

Embodiment 2

[0066] Embodiment 2: the extraction separation of compound 1, 2

[0067] 1Kg of kiwifruit leaves (collected in June 2007 from the mountainous area of ​​Quzhou, Zhejiang Province) was extracted by percolation with 50L of 80% ethanol, the extracts were combined, the solvent was recovered under reduced pressure to 1L, and the solvent was saturated with petroleum ether, ethyl acetate, and water in turn. Butanol extraction, the n-butanol extracts were combined and concentrated to obtain the decolorized product. After the decolorization, the product was dissolved in methanol, filtered, and part of the filtrate was taken to pass through the Sephadex LH-20 column, and eluted with a gradient of 10% to 50% aqueous methanol solution, 50ml / part was received, and the fractions received were tracked and detected by TLC (silica gel GF254 thin-layer plate , Developing agent: n-butanol-acetic acid-water=4:1:5 upper layer, color development method: visual inspection under ultraviolet lamp 254nm...

Embodiment 3

[0068] Embodiment 3: the pharmacological experiment of compound 1, 2

[0069] 1. Hypoglycemic experiment

[0070] Spontaneous type 2 diabetic db / db mice (Nanjing University Model Animal Center) with blood glucose values ​​greater than 20 mmol / L were randomly divided into groups. The mice in the treatment group were intragastrically administered with the doses in Table 2; the mice in the positive drug group were intragastrically administered glibenclamide; the mice in the model group were intragastrically administered an equal volume of blank solvent. Animals in each group were administered once a day for 1 week continuously. Three hours after the administration on the 1st, 3rd, and 7th day, the blood was taken from the tip of the tail of the mice, and the blood glucose concentration was measured with a Maojing blood glucose meter to investigate the effect of administration on the blood glucose concentration of spontaneous type 2 diabetic db / db mice , the experimental results...

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Abstract

The invention discloses two types of flavonoid glycoside compounds which are kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(2'' ', 4'' '-O-diacetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-glucopyrose galactopyranoside and kaempferol-3-O-[alpha-L-rhamnopyranosyl-(1-3)-(4'' '-O-acetyl-alpha-L-rhamnopyranosyl)-(1-6)]-beta-D-glucopyrose galactopyranoside respectively. The invention also discloses a method for preparing the two types of flavonoid glycoside compounds. Moreover, pharmacological tests prove that the two types of flavonoid glycoside compounds have quite good effects of reducing blood sugar and blood fat and resisting oxidation and cerebral ischemia, and thus, the flavonoid glycoside compounds of the invention can be used for preparing medicaments, food and cosmetics for preventing and treating diabetes, reducing blood fat and resisting oxidation and cerebral ischemia.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to two flavonoid glycoside compounds; the invention also relates to a preparation method of the two flavonoid glycoside compounds and the application of the two flavonoid glycoside compounds in the field of medicine. Background technique [0002] Modern research shows that reactive oxygen species (ROS) refers to the general term for a class of chemically very active substances that are produced by redox reactions and contain oxygen on their molecules, including superoxide anion radicals, hydrogen peroxide, hydroxyl radicals, etc. . Active oxygen, especially free radicals, has high activity and strong oxidation reaction ability, and can attack biomacromolecules in the body through oxidation, such as nucleic acid, protein, sugar and lipid, etc., causing these substances to undergo peroxidative denaturation, Cross-linking and breakage will cause damage to cell structure and function,...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P35/00A61P9/10A61P3/10A61P39/06A61P3/06A61P25/28A61P27/12A61P27/02A61P1/16A23L1/30A61K8/60A61Q19/08
Inventor 凌昌全辛海量苏永华吴迎春盛佳钰周俊
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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