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Oligopeptide B and its antibacterial application

An oligopeptide and anti-bacterial technology, applied in the field of biomedicine, can solve the problems of drug resistance of pathogenic bacteria threatening human health and clinical treatment of diseases, and achieve the effect of convenient synthesis and broad antibacterial spectrum

Inactive Publication Date: 2012-03-07
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, due to the widespread use of antibacterial drugs, some new problems have arisen, such as the emergence of corresponding drug-resistant pathogenic strains, that is, the emergence of bacterial drug resistance, and the drug resistance of pathogenic bacteria has become an increasingly serious threat Clinical treatment of human health and disease

Method used

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  • Oligopeptide B and its antibacterial application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Design and synthesis of oligopeptide B of the present invention

[0015] 1. According to the current major international antimicrobial peptide databases (http: / / www.imtech.res.in / raghava / antibp / ; http: / / aps.unmc.edu / AP / main.php; http: / / www.bbcm .univ.trieste.it / ; http: / / penbase.immunaqua.com / ) the sequence of antimicrobial peptides, analyze the corresponding relationship between its primary structure and activity, and design artificial oligopeptide sequences.

[0016] 2. Use the protein analysis database http: / / www.expasy.org / to analyze the similarity of the secondary structure between the peptide sequence of known activity and the designed oligopeptide sequence, and to modify the design sequence in combination with the prediction of other physical and chemical parameters.

[0017] 3. Send the designed artificial oligopeptide sequence to a biological company for synthesis or use an automatic peptide synthesizer to synthesize the sequence. HPLC detection purity is greater tha...

Embodiment 2

[0021] Using liquid growth inhibition method to detect the antibacterial activity of the oligopeptide B of the present invention

[0022] 2.1 Materials and reagents

[0023] 1) The oligopeptide B of the present invention is synthesized by Shanghai Shenggong Biological Engineering Technology Service Co., Ltd., ampicillin (Amp) is a product of Solarbio Company, a disposable filter is a product of PALL Company of the United States, and other pharmaceutical reagents are domestically pure.

[0024] 2) Select Gram-positive bacteria: Bacillus subtilis (Ehrenberg) Cohn, Staphyloccocus aureus Rosenbach, Micrococcus luteus, Sarcina ureae, Bacillus thuringiensis (Bacillus thuringiensis), Bacillus megaterium (Bacillus megaterium); Gram-negative bacteria: Escherichia coli, Vibrio, Klebsiella pneumoniae, Serratia marcescens (Serratia marcescens), Proteus, Bacillus licheniformis; Fungi: Fusarium solani, Fusarium oxysporum, Colietetrichum lagenarium, Apple anthracnose The germ (Gloeosporium album)...

Embodiment 3

[0038] Escherichia coli was used as indicator bacteria.

[0039] The oligopeptide B of the present invention with a mass concentration of 40 mg / mL was applied to Escherichia coli for 10 min, 20 min, 30 min, 45 min, 60 min, 90 min, 120 min, and 180 min, respectively.

[0040] The inhibition curve of Escherichia coli shows that the inhibitory effect of the oligopeptide B of the present invention on the bacteria starts to play within 20 minutes, the inhibition rate can reach more than 75% after 45 minutes, and more than 90% after 90 minutes.

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Abstract

The invention discloses an oligopeptide B with antibacterial activity and with an amino acid sequence shown in SEQ ID NO.1. The invention also discloses application of the oligopeptide B in preparing antibacterial medicaments. Experiments prove that: the oligopeptide B of the invention has a broad antibacterial spectrum, and has an obvious inhibition effect on both Gram positive bacteria and Gram negative bacteria, and also plays a great role in fungus inhibition. Therefore, the oligopeptide B provided in the invention boasts significant value in antibacterial medicament development and application.

Description

Technical field [0001] The invention relates to an antibacterial peptide and its application, in particular to an oligopeptide B and its antibacterial application, and belongs to the field of biomedical technology. Background technique [0002] At present, due to the proliferation of antimicrobial drugs, some new problems have arisen, such as the emergence of corresponding drug-resistant pathogenic strains, that is, bacterial drug resistance, and the drug resistance of pathogenic bacteria has increasingly threatened Human health and clinical treatment of diseases. Therefore, finding new types of antibacterial drugs has become another strategy to solve the problem of drug resistance. [0003] Antimicrobial peptides refer to a type of small molecule peptides distributed in organisms that can resist the invasion of external microorganisms and remove mutant cells in the body. According to the number of amino acids in the peptide, the compound formed by the dehydration and condensation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P31/04A61P31/10
Inventor 张尚立张恒常宏文王萍王丽
Owner SHANDONG UNIV
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