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Method for extracting caragana sinica rehd, and application of caragana sinica rehd extract

A root extract and a technology for an extraction method are applied to the extraction method of gorse root and the application field of the extract in cosmetics, and can solve the problem of no trypsin and the like.

Inactive Publication Date: 2012-03-14
SHANGHAI BOTANOVA COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are no reports in the existing literature that Gorcia root extract and its derivatives and analogs can inhibit the activity of trypsin

Method used

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  • Method for extracting caragana sinica rehd, and application of caragana sinica rehd extract
  • Method for extracting caragana sinica rehd, and application of caragana sinica rehd extract
  • Method for extracting caragana sinica rehd, and application of caragana sinica rehd extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Extraction process of Gorcia root dried medicinal material

[0018] The genus root extract mentioned in the present invention is extracted from the root or root bark of the medicinal plant Caragana japonica through multiple reflux extractions with organic solvents. The solvent-extracted components are further purified by chromatography or fractional distillation, and the product is isolated. The crushed particle size of the gorse root is 100um-5000um; 30-90% ethanol aqueous solution is used for heating and reflux extraction; the ratio of gorse root powder to solvent is 1:10; the extraction temperature is kept between 20-90°C; the extraction The time is between 8-24 hours; the ethanol extract is decolorized with 1-40% activated carbon, concentrated, dried, and set aside. process such as figure 1 shown.

[0019] The sample liquid is further concentrated and dried to obtain the ethanol extract of Gorcia root. The appearance of the product is brown powder (i...

Embodiment 2

[0021] Example 2 Experimental study on the inhibition of trypsin activity by Gorcia root extract

[0022] experiment material:

[0023] 1. Trypsin (Trypsin from bovine pancreas, Sigma, USA, product number: T-8802)

[0024] 2. L-BAPA (Sigma, USA, item number: B3133)

[0025] 3. Hexamidine bis(isethionate) salt

[0026] 4. 96-well plate (Corning, USA, catalog number: 3599)

[0027] 5. 384-well plate (Greiner, Germany, catalog number: 781101)

[0028] 6. Other reagents: Tris (Tris), calcium chloride (analytical grade, CaCl 2 ), 36% hydrochloric acid (analytical grade), dimethyl sulfoxide (DMSO)

[0029] Reagent preparation

[0030] 1. Buffer: mix 6.05g Tris and 2.22g CaCl 2 Dissolve in 1L of distilled water, adjust the pH value to 8.2 with 6N hydrochloric acid, and store at 4°C;

[0031] 2. Enzyme: Trypsin

[0032] 1) Mother solution (1mM): Dissolve 50mg trypsin in 2.17mL distilled water;

[0033] 2) Aliquot 30uL per tube and store at -20°C;

[0034] 3) Working s...

Embodiment 3

[0065] Embodiment 3: prepare skin cream (by weight ratio)

[0066] Gorse root ethanol extract 0.1-3%

[0067] Glycerin 3-8 %

[0068] Caprylic / Capric / Triglycerides 3-8 %

[0069] Isooctyl Palmitate 3-8 %

[0070] Cetyl Alcohol / Stearyl Alcohol 3-5 %

[0071] C12-20 Alkyl Glucoside 1-3 %

[0072] Ceteareth-25 1-3 %

[0073] Polydimethylsiloxane 1-3 %

[0074] Mineral oil 3-8 %

[0075] Methylparaben 0.1-0.5%

[0076] Phenoxyethanol 0.3-0.5%

[0077] Flavor Appropriate amount

[0078] Deionized water plus 100%

[0079] Preparation method: heat de-particle water and glycerin and methylparaben in the water phase to 80°C, and simultaneously heat isooctyl palmitate, caprylic acid / capric acid / triglyceride, cetyl alcohol / stearyl alcohol, C12-20 alkyl glucoside, ceteareth-25, polydimethylsiloxane, and phenoxyethanol are heated to 80°C, and the oil phase solution is slowly added to the water phase solution while stirring, and homogeneous Stir for 2-5 minutes, turn on the vacu...

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Abstract

The invention relates to cosmetics industry, in particular to a method for extracting caragana sinica rehd and application of caragana sinica rehd extract to cosmetics. The method comprises the following steps of: mechanically grinding and crushing root or velamen of medicinal plant caragana sinica into powder; refluxing and extracting the powder by using an organic solvent for multiple times; and purifying components which are obtained through solvent extraction by chromatography or a fractionation method further, and separating to obtain the caragana sinica rehd extract. The extract has the activity of inhibiting trypsin, so the extract has the effects of preventing skin wrinkling and can be used as a natural plant additive in daily cosmetics.

Description

technical field [0001] The invention relates to the cosmetics industry, in particular to an extraction method of gorse root and the application of the extract in cosmetics. Background technique [0002] Skin is an important part of maintaining human life and health. It is in direct contact with the outside world and is the first barrier of the body. It is extremely vulnerable to damage caused by environmental factors. In addition to aging factors caused by physiological factors such as age and hormone levels, stress response, pollution, sunlight and ultraviolet radiation are all important causes of skin wrinkles. [0003] Skin aging is mainly manifested in two aspects. One is that collagen is degraded and lost under the action of various proteases, and the skin loses elasticity to form wrinkles; stains. During the aging process of the skin, trypsin and matrix metalloproteinase not only participate in the degradation of matrix protein and collagen in the skin structure, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/00A61Q19/08A61Q19/10A61Q17/04A61P17/00
Inventor 束放
Owner SHANGHAI BOTANOVA COSMETIC
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