Method for culturing human airway epithelial cells

A technology of airway epithelial cells and culture methods, applied to artificial cell constructs, animal cells, vertebrate cells, etc., can solve problems such as easy pollution, limited number of specimens, and small number of cells, and achieve adherence rate and survival Increased efficiency, guaranteed quantity and quality, and high cell purity
CN102433296AInactive Publication Date: 2012-05-02THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
Publication Date
2012-05-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for culturing human airway epithelial cells. The method comprises the following steps of: (1) acquiring human airway epithelial cells which are subjected to primary culture; and (2) acquiring human airway epithelial cells which are subjected to subculture, namely cleaning by using a phosphoric acid buffer solution when the fusion density of primary cells reaches 70 to 90 percent, adding a 0.25 percent trypsin solution, digesting at room temperature for 5 to 10 minutes, collecting cell suspension when cells are retracted and suspended, adding an equal amount of solution containing a protease inhibitor, performing centrifugal collection on the cells, inoculating in a novel culture dish containing a complete medium at the concentration of between 1 and 6*10<6> cells / ml, culturing, and thus obtaining the human airway epithelial cells which are subjected to subculture when the cells grow to a logarithmic growth phase after 3 to 5 days. The method for culturing the human airway epithelial cells has a stable technology and is high in repeatability; and the cultured cells have high purity and uniform morphology, grow well and can be subjected to continuous passage.
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Description

technical field

[0001] The invention relates to a method for culturing human airway epithelial cells, which belongs to the field of biotechnology. Background technique

[0002] The trachea or bronchi are an important part of the lower respiratory tract, and airway epithelial cells are an important defense barrier of the respiratory tract. Studies have found that repeated respiratory infections are closely related to mucosal epithelial dysfunction. It is an important research material for the pathogenesis of common diseases of the respiratory system. The biological characteristics of airway epithelial cells have not changed in vitro, which can reflect the state in vivo to a certain extent, and show the morphological characteristics and cell biological characteristics of parental tissues. Therefore, the primary culture and cell biology of airway epithelial cells in vitro The establishment of strains is an effective means to study the biological characteristics of parental tis...

Claims

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