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Animal magnetic ferritin material and its preparation method

An animal ferritin and animal magnetism technology, which is applied in the field of biomimetic synthesis of magnetic nanomaterials, can solve the problem that the product has a single composition, cannot remove in vitro mineralization by-products and ferritin aggregates, and is difficult to control dissolved oxygen and ferrous ions. Proportion and other issues, to achieve the effect of simple and feasible purification method, avoiding immunogenicity and maintaining integrity

Inactive Publication Date: 2012-05-23
INST OF GEOLOGY & GEOPHYSICS CHINESE ACAD OF SCI
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  • Claims
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Problems solved by technology

Moreover, air was used as an oxidant in previous experiments, and it was difficult to control the ratio of dissolved oxygen and ferrous ions in the actual solution, so it was difficult to keep the product composition single, and there was no post-separation step, and a small amount of in vitro mineralization by-products and iron could not be removed. protein aggregates

Method used

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  • Animal magnetic ferritin material and its preparation method
  • Animal magnetic ferritin material and its preparation method
  • Animal magnetic ferritin material and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0045] In an anaerobic glove box, 50ml of 25mM ammonium ferrous sulfate and 8.34mM of H 2 o 2 50ml, the NaOH 100ml of 100mM NaCl solution and 50mM, the empty shell porcine spleen ferritin solution ( figure 2 ) was diluted with 100mM NaCl solution to a volume of 50ml, the final protein concentration was 0.25mg / ml, and put into the cuvette. First, use a heating mantle to heat the protein solution in the reaction cup to 65°C. During heating, add a stir bar and stir quickly with magnetic force to ensure that the temperature of the solution is uniform. After the temperature is constant for 15 minutes, use 50mM NaOH to adjust the pH value of the protein solution through a constant pH titrator. to 8.5, then add ferrous ammonium sulfate and H 2 o 2 Each 2.245ml, the iron content of final ferritin is 2300 iron atoms. Then, the synthesized magnetic porcine spleen ferritin is subjected to molecular sieve chromatography and electrophoresis to check the protein types corresponding to...

Embodiment 2

[0047] Dilute the purified empty-shell porcine spleen ferritin solution to 50 ml with deoxygenated 100 mM NaCl solution, and put it into a reaction cup. The final protein concentration is 1mg / ml. First, heat the protein solution in the reaction cup to 37°C with an electric heating mantle. During heating, add a stirrer and stir quickly with magnetic force to ensure that the solution temperature is uniform. After the temperature is constant for 15 minutes, use 50mM NaOH to titrate with constant pH The instrument adjusts the pH value of the protein solution to 8.5, and then adds ferrous ammonium sulfate and H 2 o 2 Each 2.245ml, the iron content of final ferritin is 2300 iron atoms. All the other operations are the same as in Example 1. Figure 4 It is a transmission electron microscope (TEM) negative staining photo of magnetic porcine spleen ferritin in an aggregated state before molecular sieve separation.

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Abstract

The invention relates to an animal magnetic ferritin material comprising a complete animal ferritin shell and a magnetic nanoparticle core, the diameter of which ranges from 1 nanometer to 8 nanometers. With dispersibility and dissolvability in water and polar solvents, the core comprises magnetite or maghemite. The preparation method of the animal magnetic ferritin material consists of: 1) subjecting an animal offal to pulp refining, filtering and centrifugation to remove other proteins, then conducting nickel column and molecular sieve chromatography to separate monosomic natural ferritin; 2) in an acidic condition, carrying out reduction and denucleation to the natural ferritin so as to obtain denulceated ferritin; 3) taking hydrogen peroxide as an oxidizing agent, and adding it to a denulceated ferritin solution for reaction to synthesize animal magnetic ferritin, with the mole ratio of Fe(II) / H2O2 greater than or equal to 2:1, the pH controlled at 7.1-11, and the temperature maintained at 25-70DEG C; 4) separating the obtained animal magnetic ferritin so as to obtain monodispersed and nonmagnetic interacting animal magnetic ferritin particles. The invention also provides a preparation method.

Description

technical field [0001] The invention relates to the technical field of biomimetic synthesis of magnetic nanometer materials. In particular, it relates to a biological protein mineralization template to synthesize an animal magnetic ferritin material with a diameter less than 10nm. [0002] The invention also relates to a method of preparing the above-mentioned materials. Background technique [0003] Superparamagnetic magnetite (Fe 3 o 4 ) or maghemite (γ-Fe 2 o 3 ) particles have high saturation magnetization, nano-size effect, and good mechanical properties and chemical stability. Therefore, it is widely used in magnetic recording storage, magnetic fluid, catalysis and biomedicine, such as: magnetically targeted drugs, magnetic resonance imaging enhancement agents, cell marker separation, DNA separation, disease diagnosis, and other technical fields. However, particle size distribution, mineral phase and the presence or absence of interaction between particles are im...

Claims

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Application Information

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IPC IPC(8): C07K14/79
Inventor 田兰香曹长乾潘永信
Owner INST OF GEOLOGY & GEOPHYSICS CHINESE ACAD OF SCI
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