Agar protococcus biologic fertilizer and preparation method thereof

A technology of bio-fertilizer and agar, applied in fertilization equipment, fertilizer mixture, application, etc., can solve the problems of severe process conditions, single active ingredient of the product, and inability to effectively break algal bodies, so as to achieve simple preparation process and improve stress resistance , The effect of fast absorption and conduction speed

Active Publication Date: 2012-07-18

NINGBO UNIV

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Problems solved by technology

[0005] Another search of the Chinese patent literature database shows that CN1269339A, CN1473798A, CN1594227A, etc. utilize chemical methods to produce seaweed fertilizers, but the active ingredients of the products are single, and the process conditions are severe; wh...

Abstract

The invention relates to an agar protococcus biologic fertilizer and a preparation method thereof. The preparation method comprises the following steps: taking agar protococcus as a raw material; performing the processes of super-micro crushing, water extraction, secondary bacteria glycolysis, sterilizing, and the like; dissolving out the effective components including polysaccharide, growth hormone, protein, mineral substance, vitamin, and the like; and preparing safe and harmless natural fertilizer with maximum obtained and kept active substances of protococcus. The preparation method has the advantages of simple and easily performed process and mild conditions. Meanwhile, the microbial enzyme for degrading agar is combined and is used for performing enzymolysis on cell wall polysaccharide and generating oligosaccharide exciton with plant arousing resistance, so that the double effect of boosting growth and stress tolerance is achieved. No any chemical matter is added into the agar protococcus biologic fertilizer provided by the invention; the product is natural, green, safe and easily absorbed by plants; the sprouting percentage of the plants is increased; the growth of the plants is quickened; and the stress tolerance of the plants is improved.

Application Domain

Fertilizer mixtures

Technology Topic

FertilizerOn cells +15

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Examples

Experimental program(3)
Effect test(2)

Example Embodiment

[0023] Example 1

[0024] Take an appropriate amount of finely pulverized laver, add water according to algae: water = 1:2 (v/v), and stir and extract at 60°C for 12 h. Activate the agar-degrading bacteria, expand it into a 1 l Erlenmeyer flask, and culture for 24h on a shaker, so that the bacterial density in the obtained fermentation broth reaches 20g/L (wet weight), and the agar-degrading enzyme activity in the fermentation broth To reach 300U/ml, add the laver extract together with the algae to the fermentation broth at a ratio of 1:10, continue the shaking table reaction for 36 hours, and then suction filter to remove the residue, and then add the resulting degradation liquid at a ratio of 1:1.5 The activated fermentation bacteria liquid is subjected to secondary bacterial digestion for 48 hours, and the obtained degradation liquid is the biological fertilizer of the present invention.

Example Embodiment

[0025] Example 2

[0026] Take an appropriate amount of finely pulverized Gelidium aquilinum, add water according to algae:water=1:3 (v/v), and stir and extract at 55°C for 24h. Activate the agar-degrading bacteria, expand it into a 3 l Erlenmeyer flask, and culture for 28h on a shaker to make the bacteria density in the obtained fermentation broth reach 32g/L (wet weight), and the agar-degrading enzyme activity in the fermentation broth When it reaches 350U/ml, add Gelidium amansii extract together with the algae to the fermentation broth at a ratio of 1:15, continue shaking for 36 hours, and then suction and filter to remove the residue, and the resulting degradation liquid will be at a ratio of 1:1.5 The activated fermentation bacteria liquid is added to carry out the second bacterial digestion for 36 hours, and the obtained degradation liquid is the biological fertilizer of the present invention.

Example Embodiment

[0027] Example 3

[0028] Take an appropriate amount of finely pulverized Gracilaria, add water according to algae: water = 1:3 (v/v), and stir and extract in the reactor at 60°C for 24 hours. Activate the agar-degrading bacteria, expand it to a 100 l fermenter, and ferment for 36 hours, so that the bacteria density in the obtained fermentation broth reaches 18g/L (wet weight), and the agar-degrading enzyme activity in the fermentation broth reaches 250U/ml, add the Gracilaria extract together with the algae to the fermentation broth at a ratio of 1:10, continue to stir and react for 36h, and then remove the residue by suction filtration, and then add the resulting degradation solution at a ratio of 1:1.5. The new fermentation tank containing the fermentation bacteria liquid carries out the second bacterial hydrolysis, and the fermentation time is 48 hours, and the obtained degradation liquid is the biological fertilizer of the present invention.

[0029] The following experiments illustrate the specific application and efficacy of the collagen algae bio-fertilizer of the present invention.

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Description & Claims & Application Information

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Simple preparation process
low cost

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Agar protococcus biologic fertilizer and preparation method thereof

AI-Extracted Technical Summary

Problems solved by technology

Abstract

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Examples

Example Embodiment

Example Embodiment

Example Embodiment

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Description & Claims & Application Information

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