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Agar protococcus biologic fertilizer and preparation method thereof

A technology of bio-fertilizer and agar, applied in fertilization equipment, fertilizer mixture, application, etc., can solve the problems of severe process conditions, single active ingredient of the product, and inability to effectively break algal bodies, so as to achieve simple preparation process and improve stress resistance , The effect of fast absorption and conduction speed

Active Publication Date: 2012-07-18
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] Another search of the Chinese patent literature database shows that CN1269339A, CN1473798A, CN1594227A, etc. utilize chemical methods to produce seaweed fertilizers, but the active ingredients of the products are single, and the process conditions are severe; while CN101486612A, CN101580852A, etc. are prepared by bacterial enzyme methods, but the enzymes involved are not aimed at raw materials. Degradative enzymes of algae cell walls, so the algae cannot be effectively broken and various functional ingredients can be obtained

Method used

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  • Agar protococcus biologic fertilizer and preparation method thereof
  • Agar protococcus biologic fertilizer and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Take an appropriate amount of finely pulverized laver, add water according to algae:water=1:2 (v / v), stir and extract at 60°C for 12h. The agar-degrading bacteria are activated, expanded to 1 liter of Erlenmeyer flask, shaker culture 24h, the bacterial density in the fermented bacterium liquid that is obtained reaches 20g / L (wet weight), the activity of the agar-degrading enzyme in the fermented bacterium liquid When it reaches 300U / ml, add the laver extract and algae into the fermentation broth at a ratio of 1:10, continue the shaking table reaction for 36 hours, and then remove the residue by suction filtration, and then add the obtained degradation solution at a ratio of 1:1.5 The activated fermentation liquid is subjected to secondary bacterial hydrolysis for 48 hours, and the obtained degradation liquid is the biological fertilizer of the present invention.

Embodiment 2

[0026] Take an appropriate amount of finely crushed agaricus, add water according to algae:water=1:3 (v / v), stir and extract at 55°C for 24h. The agar degrading bacteria is activated, expanded to 3 liters of Erlenmeyer flasks, shaker culture 28h, makes the bacterium density in the fermented bacterium liquid that obtains reach 32g / L (wet weight), the activity of the agar degrading enzyme in the fermented bacterium liquid When it reaches 350U / ml, add the extract of Agaricus japonica along with the algae into the fermentation broth at a ratio of 1:15, continue the reaction in a shaking table for 36 hours, and then remove the slag by suction filtration, and then use the ratio of 1:1.5 to degrade the solution Add the activated fermented bacteria liquid to carry out secondary bacterial decomposition, the time is 36 hours, and the obtained degradation liquid is the biological fertilizer of the present invention.

Embodiment 3

[0028] Take an appropriate amount of finely pulverized Gracilaria, add water according to the ratio of algae: water = 1:3 (v / v), stir and extract in the reactor at 60°C for 24 hours. The agar degrading bacteria are activated, expanded to 100 l fermenters, fermented and cultivated for 36 hours, the bacterial density in the obtained fermented bacterium liquid reaches 18g / L (wet weight), and the activity of the agar degraded enzyme in the fermented bacterium liquid reaches 250U / ml, according to the ratio of 1:10, add the extract of Gracilaria and algae into the fermentation broth, continue to stir and react for 36h, and then remove the slag by suction filtration, and then add the obtained degradation solution into the fermentation broth according to the ratio of 1:1.5 Secondary bacteriolysis is carried out in a new fermenter containing fermentation liquid, and the fermentation time is 48 hours, and the obtained degradation liquid is the biological fertilizer of the present inventi...

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Abstract

The invention relates to an agar protococcus biologic fertilizer and a preparation method thereof. The preparation method comprises the following steps: taking agar protococcus as a raw material; performing the processes of super-micro crushing, water extraction, secondary bacteria glycolysis, sterilizing, and the like; dissolving out the effective components including polysaccharide, growth hormone, protein, mineral substance, vitamin, and the like; and preparing safe and harmless natural fertilizer with maximum obtained and kept active substances of protococcus. The preparation method has the advantages of simple and easily performed process and mild conditions. Meanwhile, the microbial enzyme for degrading agar is combined and is used for performing enzymolysis on cell wall polysaccharide and generating oligosaccharide exciton with plant arousing resistance, so that the double effect of boosting growth and stress tolerance is achieved. No any chemical matter is added into the agar protococcus biologic fertilizer provided by the invention; the product is natural, green, safe and easily absorbed by plants; the sprouting percentage of the plants is increased; the growth of the plants is quickened; and the stress tolerance of the plants is improved.

Description

technical field [0001] The invention relates to a seaweed bio-fertilizer, in particular to an agar-based algae bio-fertilizer mainly producing agar-like polysaccharides and a preparation method thereof. Background technique [0002] In recent years, with the long-term and unreasonable large-scale use of chemical fertilizers, soil nutrients have become unbalanced and the environment has been polluted. Therefore, many countries strongly advocate the application of organic fertilizers and biological fertilizers to develop green agriculture. Seaweed is a typical healthy natural green plant with high protein, low fat, high fiber and high minerals. Among them, high protein can become the natural nitrogen source needed for plant growth; carbohydrates can be developed through technological research to develop oligosaccharide elicitors, which can trigger natural immune defense signals in plants and enhance various stress resistance properties of plants (for Systemic resistance to p...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 杨敏陈海敏严小军
Owner NINGBO UNIV
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