Preparation method and application of drug-carrying keratin film

A keratin and drug-loading technology is applied in the field of preparation of drug-loaded keratin membranes, which can solve the problems of high brittleness, toxic and side effects of chemical cross-linking agents, large pollution, etc., and achieves improved tensile strength, broad application prospects, and environmental protection. The effect of pollution

Inactive Publication Date: 2012-07-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, membrane materials prepared from a single keratin have disadvantages such as high brittleness and insufficient mechanical strength, which hinder their application in various aspects. It is usually necessary to use crosslinking agents (such as formaldehyde, epoxy resin) to improve their mechanical properties, but due to The toxic and side effects of chemical cross-linking agents, as well as the problems of high energy consumption and pollution in chemical reactions, make this traditional modification method more and more restricted.
At the same time, the film made of keratin is used as the drug carrier, and the prepared film has a large drug loading capacity, but the drug release rate is too fast

Method used

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  • Preparation method and application of drug-carrying keratin film
  • Preparation method and application of drug-carrying keratin film

Examples

Experimental program
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Effect test

Embodiment 1

[0015] The preparation method of embodiment 1 drug-loaded keratin film

[0016] After the wool fabric is washed and dried, it is degreased with a 4:1 mixture of chloroform and methanol, and the degreased wool is dissolved in 0.4mol / L sodium bisulfite, 7mol / L urea and 0.15mol / L dodecylsulfuric acid In the sodium mixed solution, the dissolution temperature is 70°C, the dissolution time is 4 hours, and the pH value is kept between 6-7. The resulting solution is dialyzed in distilled water for 2 days to obtain an aqueous solution of keratin. Concentration concentration (w / v) to 10%; the weight ratio of keratin solution and plasticizer glycerin is 1.5%, adding DTT content is 10mmol / L, adjusting the pH value to 7.5 with NaOH, adding 1% medicine, and adding 30U glutamine per gram of keratin Amide transaminase, stirred evenly, and kept at 37°C for 8 hours, degassed, poured on a glass plate lined with polyethylene film, dried naturally at room temperature for 24 hours, and peeled off t...

Embodiment 2

[0017] The preparation method of embodiment 2 drug-loaded keratin film

[0018] After the wool fabric is washed and dried, it is degreased with a 4:1 mixture of chloroform and methanol, and the degreased wool is dissolved in 0.6mol / L sodium bisulfite, 9mol / L urea and 0.05mol / L dodecylsulfuric acid In the sodium mixed solution, the dissolution temperature is 50°C, the dissolution time is 7 hours, and the pH value is kept between 6-7. The resulting solution is dialyzed in distilled water for 2 days to obtain an aqueous solution of keratin. Concentration concentration (w / v) to 4%; the keratin solution and the plasticizer glycerin are 0.5% by weight, the content of DTT is added to 20mmol / L, the pH value is adjusted to 7 with NaOH, 2% of the drug is added, and 30U glutamine is added per gram of keratin Amide transaminase, stirred evenly, and kept at 37°C for 14 hours, degassed, poured on a glass plate lined with polyethylene film, dried naturally at room temperature for 24 hours, a...

Embodiment 3

[0019] The preparation method of embodiment 3 drug-loaded keratin film

[0020] After the wool fabric is washed and dried, it is degreased with a 4:1 mixture of chloroform and methanol, and the degreased wool is dissolved in 0.6mol / L sodium bisulfite, 8mol / L urea and 0.06mol / L dodecylsulfuric acid In the sodium mixed solution, the dissolution temperature is 65°C, the dissolution time is 5 hours, and the pH value is kept between 6-7. The resulting solution is dialyzed in distilled water for 3 days to obtain an aqueous solution of keratin. Concentration concentration (w / v) to 6%; the keratin solution and the plasticizer glycerin are 1.5% by weight, the pH value is adjusted to 8.5 with NaOH, 3% of the medicine is added, 30U transglutaminase is added per gram of keratin, stirred evenly, and Keep it warm at 55°C for 3 hours, pour it on a glass plate lined with polyethylene film after degassing, dry naturally at room temperature for 24 hours, and peel off the film to obtain a drug-l...

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Abstract

The invention discloses a preparation method and application of a drug-carrying keratin film. As cross link is performed on wool keratin through catalysis of glutamine transaminase to form a film, the tensile strength and water-soluble stability of the keratin film are improved; and when the cross-linked keratin film serves as a drug carrier, the release rate of a drug can be delayed and reduced by 20%, thus the drug-carrying keratin film has good application prospect.

Description

technical field [0001] The invention relates to a method for preparing a drug-loaded keratin film and its application, in particular to a method for preparing a keratin film with a slow-release function and its application. Background technique [0002] In recent years, in the field of biomedical polymer materials research, the development and research of drug controlled release polymer carriers has become one of the research hotspots in this field, and it is also a new field of biomedical engineering development. The controlled release of drugs is characterized by effective control of the medical dose of drugs, reducing their toxic side effects, reducing drug resistance in the human body, and improving drug stability and effective utilization. Drug controlled release polymer carriers can play an important role in the development of new drug dosage forms including targeted delivery dosage forms, especially in the development and development of anticancer drug dosage forms wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K31/196A61K9/70A61P29/00C08J3/24C08L89/00
Inventor 崔莉宫俊王平范雪荣王强
Owner JIANGNAN UNIV
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