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pH fluorescent sensor based on allyl calcein and preparation method thereof

A fluorescent sensor, allyl calcium technology, applied in the field of fluorescent sensors to achieve excellent selectivity, good stability, and prevent leakage

Inactive Publication Date: 2012-12-19
JIANGSU RADIO & TV UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is well known that many sensors face great challenges in terms of sensitivity, stability, selectivity and repeatability

Method used

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  • pH fluorescent sensor based on allyl calcein and preparation method thereof
  • pH fluorescent sensor based on allyl calcein and preparation method thereof
  • pH fluorescent sensor based on allyl calcein and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the synthesis of allyl calcein

[0021] Allyl Calcein Synthetic Step Reference: The synthetic route synthesis in the Journal of Wuhan Institute of Chemical Technology, 1998:V20 15-17, the specific steps are as follows:

[0022] (1) Weigh 500mg of calcein, dissolve it in NaOH solution with pH=13, add calcium chloride at a ratio of 1:1.1 (molar ratio), after it is completely dissolved, adjust the pH with NaOH to make the pH value greater than 12. Add 0.4ml bromopropene and add 0.1ml 18-crown-6 ether at the same time, and react on DF-101B collector type constant temperature magnetic stirrer at 60°C for 3 hours.

[0023] (2) Adjust the reacted solution to acidity with HCl. After the solution turns yellow, put it in the refrigerator to cool. The desired product (MS: 657) can be obtained by vacuum drying at 70°C for 6 hours after suction filtration the next day.

[0024] Under alkaline conditions, the hydrogen ions on the 5 carboxyl groups of allyl calcein ar...

Embodiment 2

[0026] The preparation and determination of the fluorescent sensor include the following steps:

[0027] 1. Silanization of slides: slides (diameter 12.5 mm) were soaked in chromic acid lotion for 40 minutes, then soaked in 3% hydrofluoric acid for 30 minutes, and then rinsed with distilled water so that they were no longer stained with 3 % hydrofluoric acid, then add 10% hydrogen peroxide and soak for 30 minutes. Then rinse with distilled water. Measure 0.2ml propyl methacrylate with a dry pipette, 2ml0.2mol l -1 Mix acetic acid-sodium acetate buffer solution with a pH of 3.6 and 8 ml of distilled water, and stir for 10 minutes to completely dissolve the propyl methacrylate to prepare a silylation solution. (Except for the beaker used for immersion in the chromic acid lotion, the other containers used are all plastic containers) The slides are immersed in this solution for 2-3 hours, then washed with distilled water, and dried at room temperature for later use.

[0028] 2....

Embodiment 3

[0031] Embodiment 3: Determination of correction equation

[0032] Solutions with different pH values ​​are B-R buffer solutions, prepared with phosphoric acid, acetic acid, boric acid, and 0.2mol l -1 NaOH solution to adjust the pH to the desired value.

[0033] The photoelectrode film made of allyl calcein as the fluorescent carrier was put into the polytetrafluoroethylene flow cell, and the B-R buffer solutions with different pH values ​​(pH=6.5, 6.8, 7.1, 7.4, 7.7, 8.0, 8.3, 8.6, 8.9, 9.2, 9.5, 9.8, 10.1, 10.4, 10.8, 11.1, 11.4, 11.7, 12.0), the peristaltic pump is input into the flow cell at a speed of 1.5ml / min, at the maximum excitation wavelength of 505nm and the maximum emission wavelength of 526nm Measure the fluorescence intensity (I) of the solution, and use Sigmaplot software to make a straight line fitting, such as image 3 As shown, the correction equation obtained is: I=-42.16pH+615.93 (R=0.9920). Therefore, we can measure the value of fluorescence intensity a...

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Abstract

The invention discloses a pH fluorescent sensor based on allyl calcein and a preparation method of the pH fluorescent sensor. The preparation method comprises the following steps: taking a fluorescent carrier in a film substrate monomer as the allyl calcein; dissolving 15mg of the allyl calcein in 0.2ml of N, N-dimethylformamide for sufficient dissolving; sequentially adding 200mg of acrylamide, 0.4ml of hydroxyethyl methylacrylate, 0.15ml of cross-linking agent triethoxy trimethyl acrylate and 0.2ml of triethanolamine; uniformly mixing to drop on a clean Teflon plate; covering a quartz slide after silanization on the Teflon plate; irradiating for 30 minutes by an ultraviolet lamp; washing by water and ethanol; and drying at room temperature. Fluorescent molecules of the pH fluorescent sensor are easy to synthesize, loss of the fluorescent carrier is effectively blocked through a covalence fixing method; and the sensor is simple in structure and expresses good stability, reproducibility and reversibility.

Description

technical field [0001] The invention relates to a fluorescent sensor, in particular to a pH fluorescent sensor based on allyl calcein and a preparation method thereof. Background technique [0002] pH measurement is relevant in many fields. There are often some difficulties when using traditional glass electrodes for pH measurement. This is because glass electrodes have high impedance, are easily damaged, cannot be used for the determination of the pH of solutions containing hydrofluoric acid, and have " Sodium error" and other defects. At the same time, due to its large size, it is not suitable for online detection of micro-areas, micro-environments and living organisms, and it is more difficult to adapt to applications in harsh environments. [0003] The optical fiber pH fluorescent sensor mainly detects the pH value of the solution by modifying the end of the optical fiber with a fluorescent sensitive film that recognizes pH. This type of sensor has been continuously d...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 秦品珠干方群胡大华牛承岗唐荣
Owner JIANGSU RADIO & TV UNIV
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