Composition for composite drug administration
A composition and drug delivery technology, applied in the directions of drug combination, non-active components of polymer compounds, and pharmaceutical formulations, can solve the problems of not being able to exert the maximum efficacy, reducing the amount of drugs, failing to achieve therapeutic effects, etc. release effect, high drug concentration, and the effect of promoting osseointegration of diabetic implants
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Embodiment 1
[0030] Preparation of insulin / PLA microspheres
[0031] Insulin / PLA microspheres were prepared by the double emulsion-solvent evaporation method: 100mg PLA was dissolved in 1ml of dichloromethane to make the organic phase (O), and then 0.1ml of insulin solution (30mg / ml) was added to the organic phase, Ultrasonic (190kW) forms colostrum (W / O); Colostrum is poured into 8ml concentration and is in the PVA aqueous solution of 1%, stirs under 1500rpm rotating speed and forms double emulsion (W / O / W), then pours 7mlPVA aqueous solution, puts Stir on a mechanical stirrer at a speed of 600rpm for 6-8 hours. After the methylene chloride is completely volatilized, centrifuge to collect the obtained microspheres. After washing with distilled water for several times, then collect by centrifugation, put in a Brookfield leak filter, and freeze-dry The insulin / PLA drug-loaded microspheres were obtained.
Embodiment 2
[0033] Preparation of insulin / PLGA microspheres
[0034] Insulin / PLGA microspheres were prepared by the double emulsion-solvent evaporation method: 100mg PLGA was dissolved in 1ml of dichloromethane to make the organic phase (O), and then 0.1ml of insulin solution (30mg / ml) was added to the organic phase, Ultrasonic (190kW) forms colostrum (W / O); Colostrum is poured into 8ml concentration and is in the PVA aqueous solution of 1%, stirs under 1500rpm rotating speed and forms double emulsion (W / O / W), then pours 7mlPVA aqueous solution, puts Stir on a mechanical stirrer at a speed of 600rpm for 6-8 hours. After the methylene chloride is completely volatilized, centrifuge to collect the obtained microspheres. After washing with distilled water for several times, then collect by centrifugation, put in a Brookfield leak filter, and freeze-dry The insulin / PLGA drug-loaded microspheres were obtained.
Embodiment 3
[0036] Preparation of Insulin / PLA Microspheres-Fibrin Glue Composite Administration Composition
[0037] The insulin / PLA microsphere-fibrin glue compound administration composition is compounded by insulin / PLA microsphere and fibrin glue, and the compounding ratio is 5mg:1ml.
[0038] The insulin / PLA microspheres were prepared in advance by the double emulsion-solvent evaporation method (Example 1). Fibrin glue is composed of mammalian fibrinogen freeze-dried powder, thrombin freeze-dried powder, potassium dihydrogen phosphate solution and calcium chloride solution after virus inactivation treatment. The drug composition can be configured and used on-site in diabetic implant surgery: first, the implant socket is prepared on the alveolar bone with a preparatory drill, and at the same time, the assistant dissolves the lyophilized fibrinogen powder in the potassium dihydrogen phosphate solution respectively 1. After dissolving the lyophilized thrombin powder in calcium chloride ...
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