Method for preparing fluorescent and magnetic resonance dual-functional nanometer super-paramagnetic particles for detecting life system

A dual-function, superparamagnetic technology, which is applied in the field of preparation of nano-superparamagnetic particles, can solve the problems of poor water solubility, low quantum yield, and high viscosity of inorganic light-emitting quantum dots, so as to facilitate separation, enhance sensitivity, eliminate apply limited effects
CN102898461AInactive Publication Date: 2013-01-30NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2013-01-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for preparing fluorescent and magnetic resonance dual-functional nanometer super-paramagnetic particles for detecting a life system. The method comprises the following steps of: preparing Fe3O4 nanoparticles, preparing 8-[(chloracetyl)amino]quinoline, and preparing QTEPA (N-(8-quinolone)-2-[3-(triethoxysilicyl)-alanyl]acetamide); and modifying Fe3O4 nanoparticles by using QTEPA to obtain the nanometer super-paramagnetic particles. In the method, fluorescent molecules are modified onto the surfaces of the nanometer super-paramagnetic particles through silicon dioxide, so that the fluorescent molecules are concentrated on the surfaces of the particles, the fluorescent identifying actions of the fluorescent molecules on metal zinc ions are enhanced, the detection lower limit of zinc ion concentration is lowered, and the feedback functions of the fluorescent molecules on zinc ions to be detected are enhanced; on the other hand, the nanoparticles have high dispersibility in an aqueous solution, so that the application limit of the fluorescent molecules due to poor water solubility is reduced and even eliminated; and meanwhile, the nanoparticles have high superparamagnetism, so that the nanoparticles can be taken as a proper T2 magnetic resonance contrast agent.
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Description

technical field

[0001] The invention relates to a method for preparing nanometer superparamagnetic particles, in particular to a method for preparing nanometer superparamagnetic particles with dual functions of fluorescence and magnetic resonance for life system detection. Background technique

[0002] Zinc is an important trace element essential to the human body, widely distributed in the cells and body fluids of the human body. Currently, it is known to contain Zn at the active center 2+ There are more than 1000 kinds of enzymes, and about 5000 proteins contain Zn in the 40000 protein structures listed in the Brookhaven protein database (PDB) in 2007. 2+ . Zn in vivo 2+ The detection and analysis of protein is of great significance to the study of protein structure and function, DNA and RNA synthesis, gene expression, metabolism and the occurrence of neurodegenerative diseases (such as Alzheimer's disease, etc.). However, Zn 2+ There are no empty d orbitals and unpa...

Claims

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