Trichoderma viride stain and application thereof in cucumbers

A technology of Trichoderma viride and strains, applied in the direction of application, microbe-based methods, chemicals for biological control, etc., can solve problems that affect the economic benefits of farmers, the health of the masses, excessive pesticide residues, and environmental pollution, and achieve good results. Application prospects, high-efficiency control, and wide-spectrum effects

Inactive Publication Date: 2014-02-12
宁国市百立德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical pesticides can prevent and control the main diseases of cucumbers, long-term use will easily cause excessive pesticide residues and environmental pollution, which will affect the economic benefits of farmers and the health of the masses
At the same time, the above-mentioned chemical pesticides are generally highly resistant

Method used

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  • Trichoderma viride stain and application thereof in cucumbers
  • Trichoderma viride stain and application thereof in cucumbers
  • Trichoderma viride stain and application thereof in cucumbers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Isolation and purification of Trichoderma viride (T.viride) WLTR16 (CGMCC No.6671)

[0030] Trichoderma viride (T.viride) WLTR16 (CGMCC No.6671) of the present invention is obtained from the mushrooms in Wulian County by using the method of streaking on a plate to separate and obtain, and the separation method is as follows:

[0031] ⑴Isolation of Trichoderma strains: Take seriously damaged mushroom bags, first use 75% alcohol to treat them locally for 1 minute, and then treat them with ultraviolet light on the ultra-clean workbench for 20 minutes, cut the treated parts with a sterilized blade, and inoculate A small amount of Trichoderma spores were picked up with a needle, streaked on a PDA plate, and the growth of the colony was observed regularly. Then, the Trichoderma strain was purified by the streaking method on a plate, and transferred to a PDA test tube slant for storage for later use.

[0032] (2) Screening of Trichoderma strains with high efficiency agains...

Embodiment 2

[0042] 1. Trichoderma viride fermentation process

[0043] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0044] A large amount of solid fermentation medium formula (mass percentage):

[0045] Solid material: corn cob 60%, bran 40%

[0046] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.5% calcium sulfate, 4% ammonium sulfate, 0.05% chloramphenicol, and the rest is water.

[0047] The solid-to-liquid ratio is 1:1.8 (mass ratio)

[0048] Mass solid fermentation process of Trichoderma viride (T.viride) WLTR16 (CGMCC No.6671):

[0049] ① Strain seed liquid culture: Pick a small amount of spores of Trichoderma viride (T.viride) WLTR16 (CGMCC No.6671) from the slant of the test tube, transfer them to PDB liquid medium, and culture on a shaker at 28°C for 3-5 days. This is the seed solution.

[0050] ②Cultivation of solid production strains: inoculate 10% of the seed solution into solid medium (500mL Er...

Embodiment 3

[0053] This example provides related experiments on the control and yield increase of Trichoderma viride (T.viride) WLTR16 (CGMCC No.6671) raw powder on cucumber gray mold.

[0054] 1. Drugs to be tested

[0055] Trichoderma viride (T.viride) WLTR16 (CGMCC No.6671) raw powder (prepared in Example 2); 40% imidaemycin wettable powder (produced by Shandong Shouguang Shuangxing Pesticide Co., Ltd., commercially available).

[0056] 2. Test crops and control objects

[0057] The test crop is cucumber, and the variety is Changchun mici;

[0058] The control object is Botrytis cinerea.

[0059] 3. The situation of the test site

[0060] The test site is located in the Shouguang cucumber greenhouse planting base in Shandong Province. The gray mold of cucumber in the greenhouse has been serious in recent years, and it is difficult to control. The experiment was conducted in a cucumber greenhouse of 1.2 mu. The soil quality is light loam, the organic matter content is 1.08%, and the ...

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Abstract

The invention discloses a Trichoderma viride strain and application thereof in cucumbers. The Trichoderma viride stain is WLTR16, and the collection number is CGMCC No.6671. The strain and biological control preparation comprising the strain can efficiently control cucumber diseases. The experiment indicates that the Trichoderma viride stain WLTR16 has the characteristics of fast growth, large spore production quantity, wide action spectrum and the like, can quickly and massively implement colonization in cucumber leaves and roots, and therefore, has wide application prospects. The biological control preparation prepared from the Trichoderma viride WLTR16 can efficiently control the cucumber diseases and effectively enhance the cucumber yield.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a Trichoderma viride capable of efficiently preventing and treating gray mold of cucumber, blight and Sclerotinia and its use, and a biological control agent prepared by using the Trichoderma viride. Background technique [0002] Cucumber is one of the most important vegetable varieties in my country, and the current cultivation area has exceeded 1 million hectares, ranking first in the world. However, with the expansion of cucumber cultivation area and the diversification of cultivation methods, the ecological environment of its vegetable fields has undergone great changes, making various diseases more and more serious, and the drug resistance of pathogenic bacteria has also been continuously upgraded, which has become a constraint to increase cucumber production. major factor. According to reports, there are more than 50 kinds ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 牛赡光王清海刘幸红段春华戴宝
Owner 宁国市百立德生物科技有限公司
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