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Method for testing content of main components in Rhodiola rosea extracts

A determination method and technology of Rhodiola, applied in the directions of measuring device, material separation, analysis of materials, etc., can solve the problems of complex plant sources of Rhodiola, large differences in the composition of Rhodiola, and different cultivars, and achieve quality control. Rigorous, reducing the number of experiments, the effect of clear ingredients

Active Publication Date: 2013-03-27
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the quality evaluation of Rhodiola rosea extract. The quality control of Rhodiola rosea is only based on the content of salidroside. Rhodiola is different, and the components of Rhodiola from different sources are quite different, and these evaluation methods have certain defects.

Method used

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  • Method for testing content of main components in Rhodiola rosea extracts
  • Method for testing content of main components in Rhodiola rosea extracts
  • Method for testing content of main components in Rhodiola rosea extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] [Example 1] Preparation of Rhodiola Rosea Extract

[0056] Take 10kg of Rhodiola rosea (Rhodiola rosea content ≥ 1.0%) medicinal material produced in Tibet, decoct it with 8 times the weight of water for 2 hours and take the filtrate for the first time; Filtrate, the two filtrates were combined and concentrated under reduced pressure to 10L to obtain concentrate I. Purify the concentrated solution I with a macroporous resin, the loading ratio is 1g rhodiola medicinal material: 2g resin; then first elute with water, discard the water, then elute with 20% ethanol, collect 20% ethanol for elution liquid. Concentrate the eluate to a density of 1.05-1.10 to obtain Concentrate II. Then the concentrate II was precipitated with 80% ethanol for 24-32 hours to obtain a supernatant, and the precipitate was discarded. Concentrate, dry and pulverize the alcohol precipitation supernatant to obtain the rhodiola rosea extract.

Embodiment 2

[0057] [Example 2] The detection conditions for screening the content of gallic acid, salidroside, tyrosol and p-coumaric acid in the Rhodiola rosea extract.

[0058] Preparation of Rhodiola rosea extract: prepared according to the method described in Example 1, the batch number is 100702.

[0059] Screening of chromatographic conditions

[0060] Investigated mobile phase systems such as acetonitrile-water, acetonitrile-0.1% phosphoric acid, acetonitrile-0.3% glacial acetic acid, methanol-water, methanol-0.1% phosphoric acid, and the results are as follows: figure 1 As shown, through test comparison, it is finally determined that the content determination of the above four components is completed by acetonitrile-0.3% glacial acetic acid gradient elution.

[0061] Investigated different column temperatures of 25°C, 30°C, and 40°C, the results are as follows figure 2 shown; and the influence of different flow rates of 0.5, 0.7, 1.0, 1.5mL / min on the separation of each compone...

Embodiment 3

[0069] [Example 3] Content detection of gallic acid, salidroside, tyrosol and p-coumaric acid in Rhodiola rosea extract Chromatographic conditions and system adaptability:

[0070] Column is Phenomenex Luna C 18 Column (4.6mm×250mm, 5μm), the mobile phase is acetonitrile (A)-0.3% glacial acetic acid solution (B), the elution program is: 0~5min, 9%A; 5~30min, 9%~25%A , 30~35min, 25%~40%A; column temperature: 40℃; volume flow: 0.7mL min -1 ; Detection wavelength: 275nm, 308nm; Injection volume 10μl. Take the solution of the test product and inject it under the above-mentioned chromatographic conditions for analysis. The resolution of each component to be tested and the adjacent peak in the sample is greater than 1.5, the tailing factor is between 0.95 and 1.05, and the number of plates is calculated according to Rhodiola rosea. Glycoside calculations are greater than 20,000. Chromatograms such as Figure 8 shown.

[0071] Preparation of reference solution

[0072] Take app...

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Abstract

The invention relates to a method for testing the contents of gallic acid, salidroside, tyrosol and p-coumaric acid in Rhodiola rosea extracts. The method comprises the steps of respectively preparing a gallic acid reference sample solution, a salidroside reference sample solution, a tyrosol reference sample solution and a p-coumaric acid reference sample solution and preparing a Rhodiola rosea extract test solution; testing the content of the reference sample solutions with the chromatographic column of Phenomenex Luna C18 (4.6mm*250mm, 5mum), the moving phase of acetonitrile-0.3% glacial acetic acid, the column temperature of 40 DEG C, and the volume flow rate of 0.7mL.min-1 respectively at the wavelengths of 275nm and 308nm, recording the chromatogram map and calculating the contents of gallic acid, salidroside, tyrosol and p-coumaric acid in the Rhodiola rosea extract test solution.

Description

technical field [0001] The invention relates to the detection field of traditional Chinese medicinal materials, in particular to a detection method of the traditional Chinese medicinal material Rhodiola rosea. Background technique [0002] Rhodiola grandiflora is the dry root and rhizome of Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba plant in the sedum family Rhodiola. medicinal resources. my country is the distribution center of Rhodiola grandiflora, with a large storage capacity. The plant contains salidroside, tyrosol, rhodiola rhodiola, polyphenols and polysaccharides. Modern pharmacological studies have shown that Rhodiola has anti-aging, anti-oxidative hemolysis, anti-radiation and other activities. The "Rhodiola rosea fever" in recent years has made Rhodiola rosea extracts widely used, and its quality is the premise to ensure the efficacy. There are many literature reports on the determination of active ingredients in Rhodiola rosea medicinal materials by HPLC: ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/08
Inventor 萧伟王振中毕宇安李家春林夏胡军华秦建平
Owner JIANGSU KANION PHARMA CO LTD
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