Grain-saving and environmentally-friendly microbial fermented feed and manufacturing method thereof

A microbial fermentation and feed technology, which is applied in the field of livestock and poultry feed, can solve the problems of difficult to control fermentation degree, low nutritional value, and easy to contaminate bacteria and mildew, so as to reduce breeding costs, stabilize product quality, and increase feed intake. Effect

Inactive Publication Date: 2013-04-24
QINGDAO HAIZHONGDA BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the production methods of biologically fermented feed are mainly traditional family workshops, solid accumulation fermentation, etc., and mechanized scale continuous production has not yet been formed.
Moreover, tradition

Method used

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  • Grain-saving and environmentally-friendly microbial fermented feed and manufacturing method thereof
  • Grain-saving and environmentally-friendly microbial fermented feed and manufacturing method thereof
  • Grain-saving and environmentally-friendly microbial fermented feed and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A grain-saving and environmentally friendly microbial fermented feed is prepared by mixing and fermenting the following components according to the weight ratio: 351 parts of peanut shell powder, 32 parts of secondary powder, 20 parts of bran, 54 parts of worm sand, 500 parts of brewer's grains, 20 parts of premix, 23 parts of bacterial liquid;

[0036] The premix is ​​composed of the following components by weight fraction: 452 parts of stone powder, 330 parts of zeolite powder, 24 parts of compound multidimensional, 50 parts of calcium hydrogen phosphate, 50 parts of sprayed corn husks, 90 parts of table salt, and 2 parts of flavoring agent parts, 2 parts of sweetener;

[0037] The compound multidimensional ratio is as follows:

[0038] Vitamin A 20000IU / kg Vitamin D 3 10000IU / kg

[0039] Vitamin E 110mg / kg Vitamin K 3 10mg / kg

[0040] Vitamin B 1 8mg / kg Vitamin B 2 12mg / kg

[0041] Vitamin B 6 7mg / kg Vitamin B 12 0.2mg / kg

[0042] Biotin 25mg...

Embodiment 2

[0049] The preparation method of a kind of grain-saving and environment-friendly microbial fermented feed described in embodiment 1, concrete process is as follows:

[0050] 1) Preparation of bacterial solution

[0051] A. Preparation of Pichia strain: inoculate the preserved strains into 250mL eggplant bottles filled with 70-80mL solid slant potato medium, and cultivate them in a constant temperature incubator at 30°C for 3 days; Rinse down the bacteria with sterile water, and repeatedly blow and suck to mix the bacteria and sterile water evenly to obtain a bacterial suspension. Put 3% of the inoculum into a 500mL triangular flask containing 300mL of YPD medium, and shake at a constant temperature of 30°C. Bed culture for 24 hours, rotating speed 220rpm; transfer 3% inoculum amount into a 500mL Erlenmeyer flask containing 300mL fermentation medium, culture in a constant temperature shaker at 30°C for 24 hours, rotating speed 220rpm;

[0052] The formula of the potato medium ...

Embodiment 3

[0066] The grain-saving environmental protection microbial fermented feed nutrient composition determination in embodiment 1:

[0067] 1. The crude protein content in the fermented feed was 16%; the content of three active substances: ① The free amino acid content was 47.09 g / kg, and the fermentation increase rate was 111.26%. ②The active peptide content was 0.81 g / kg, and the fermentation increase rate was 153.13%. ③The water-soluble protein content was 11.32 g / kg, and the fermentation increase rate was 220.68%.

[0068] 2. Three kinds of hydrolase activities in the fermented feed: ① The protease activity was 546.35 U, and the fermentation increase rate was 264.09%. ②The cellulase activity was 5942.67U, and the fermentation increase rate was 94.06%. ③The activity of phytase was 106.45U, and the fermentation rate was 300.04%.

[0069] test methods

[0070] Determination of protein content

[0071] Crush and sieve the dried sample, weigh 0.5000g into a small Erlenmeyer flas...

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Abstract

The invention relates to a grain-saving and environmentally-friendly microbial fermented feed which is formed by uniformly mixing and fermenting the following components in part by weight: 351 parts of peanut shell powder, 32 parts of wheat middling, 20 parts of wheat bran, 54 parts of insect sand, 500 parts of brewer's grain, 20 parts of premix compound and 23 parts of fungi, wherein the premix compound comprises the following components in part by weight: 452 parts of mountain flour, 330 parts of zeolite powder, 24 parts of compound multi-vitamin, 50 parts of calcium hydrophosphate, 50 parts of guniting corn husk, 90 parts of salt, 2 parts of flavoring agent and 2 parts of sweetening agent. The grain-saving and environmentally-friendly microbial fermented feed has the benefits that cheap agricultural leftovers are utilized and are fermented through microbial solids, wastes are changed into valuables, and a new generation of total-nutrient grain-saving and environmentally-friendly microbial fermented feed integrating nutrition, disease prevention, material saving and ecology is formed.

Description

technical field [0001] The invention relates to feed for livestock and poultry, in particular to a grain-saving and environment-friendly microbial fermented feed and a preparation method thereof. Background technique [0002] There are about 200 billion tons of cellulose resources in the world every year, and about 10 billion tons in my country, of which about 800 million tons are corn stalks. Most of these straw resources are used for fuel and fertilizer, which is a waste of resources. If we ferment 40% of the straw and turn it into feed, we can obtain feed grain equivalent to 100 million tons, which is about half of the current national annual feed grain consumption. [0003] At present, the feed and breeding industry is facing such outstanding problems as large market turmoil, prominent conflicts between humans and animals competing for food, a large amount of straw resources that have not been rationally developed and utilized, high chemical input of livestock and poultr...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/06A23K10/38
CPCY02P60/87
Inventor 栾锦涛陈继伟黄玲玲张盼盼
Owner QINGDAO HAIZHONGDA BIOTECH DEV
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