Preparation method and application of unmarked immunosensor for rapidly detecting clenbuterol

An immune sensor and clenbuterol technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of difficult operation, expensive instruments, and low false positive rate, and achieve enhanced adsorption and immobilization, high selectivity detection, The effect of improving sensitivity
CN103063833AInactive Publication Date: 2013-04-24UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2013-04-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method and application of an unmarked immunosensor for rapidly detecting clenbuterol. Clenbuterol means a medicine of a beta-stimulant type instead of a specific medicine, and has the functions of prompting lean meat growth and suppressing fat meat growth as well as extremely serious side effects, and when a large amount of clenbuterol is taken, the cardiovascular system of people can be damaged and severe neurological symptoms can be caused. According to the invention, a gold-silver-sulfide nano composite material modified electrode with a core-shell structure which combines the excellent characteristics of nano gold and nano silver sulfide is adopted for preparing the unmarked immunosensor, and a clenbuterol antibody is well immobilized, so that the unmarked immunosensor is low in cost, high in sensitivity, good in specificity, rapid in detection and simple to prepare.
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Description

technical field

[0001] The invention relates to a preparation method and application of a label-free immunosensor for rapid detection of clenbuterol. Specifically based on gold-silver sulfide (Au-Ag 2 S) A label-free electrochemical immunosensor for rapid detection of clenbuterol constructed from core-shell nanocomposites, which belongs to the technical field of new nano functional materials and biosensors. Background technique

[0002] Electrochemical immunosensors are widely used in clinical diagnosis, environmental analysis, drug analysis, food safety detection and other fields due to their high sensitivity, good specificity, and easy operation. The most critical technology for the preparation of electrochemical immunosensors with superior performance is the effective immobilization of immune molecules and the improvement of sensitivity, reproducibility, and persistence.

[0003] Gold (Au) nanoparticles have been widely used in the research of immunosensors due to their...

Claims

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