Preparation method and application of unmarked immunosensor for rapidly detecting clenbuterol
An immune sensor and clenbuterol technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of difficult operation, expensive instruments, and low false positive rate, and achieve enhanced adsorption and immobilization, high selectivity detection, The effect of improving sensitivity
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Embodiment 1
[0045] Example 1 A preparation method of a label-free immunosensor for rapid detection of clenbuterol:
[0046] (1) Treatment of the glassy carbon electrode: the glassy carbon electrode with a diameter of 4 mm was polished with 1.0, 0.3 and 0.05 μm aluminum oxide polishing powder in sequence, ultrasonically cleaned with ethanol, and then rinsed with ultrapure water to make the glassy carbon The electrode surface is mirror-like.
[0047] (2) Au-Ag 2 Preparation of S core-shell nanocomposites:
[0048] 1) 1 mmol·L in 250 mL -1 Add 60 mL of 10 mmol L to the chloroauric acid solution -1 Freezing point-sodium borohydride solution, after stirring for 1 hour, dilute 10 times to prepare nano-Au seeds;
[0049] 2) 1 mmol·L in 150 mL -1 Add 0.1 mol·L dropwise to the chloroauric acid solution -1 Ascorbic acid until colorless, then add 5 drops of Au seeds in step 1), and let it stand for 2 h to prepare Au nanoparticles;
[0050] 3) After centrifuging the Au nanoparticles prepare...
Embodiment 2
[0060] Example 2 A preparation method of a label-free immunosensor for rapid detection of clenbuterol:
[0061] (1) Treatment of the glassy carbon electrode: same as in Example 1.
[0062] (2) Au-Ag 2 Preparation of S core-shell nanocomposites:
[0063] 1) In 250 mL 1 mmol·L -1 Add 60 mL of 10 mmol L to the chloroauric acid solution -1 Freezing point-sodium borohydride solution, after stirring for 1 hour, dilute 10 times to prepare nano-Au seeds;
[0064]2) 1 mmol·L in 150 mL -1 Add 0.1 mol·L dropwise to the chloroauric acid solution -1 Ascorbic acid until colorless, then add 5 drops of Au seeds in step 1), and let it stand for 2 h to prepare Au nanoparticles;
[0065] 3) After centrifuging the Au nanoparticles prepared in step 2), redisperse them in 300 mL of 0.1 mol L -1 To the CTAB, add 30 mL 0.1 mol·L successively -1 Ascorbic acid, 5 mL 0.01 mol L -1 AgNO 3 , 30 mL 0.1 mol·L -1 NaOH aqueous solution, shaking for 1 min, and standing for 1 h, the Au-Ag nanop...
Embodiment 3
[0072] Example 3 A preparation method for rapid detection of clenbuterol label-free immunosensor, such as figure 1 shown
[0073] (1) Treatment of the glassy carbon electrode: same as in Example 1.
[0074] (2) Au-Ag 2 Preparation of S core-shell nanocomposites:
[0075] 1) 1 mmol·L in 250 mL -1 Add 60 mL of 10 mmol L to the chloroauric acid solution -1 Freezing point-sodium borohydride solution, after stirring for 1 hour, dilute 10 times to prepare nano-Au seeds;
[0076] 2) 1 mmol·L in 150 mL -1 Add 0.1 mol·L dropwise to the chloroauric acid solution -1 Ascorbic acid until colorless, then add 5 drops of Au seeds in step 1), and let it stand for 2 h to prepare Au nanoparticles;
[0077] 3) After centrifuging the Au nanoparticles prepared in step 2), redisperse them in 300 mL of 0.1 mol L -1 To the CTAB, add 30 mL 0.1 mol·L successively -1 Ascorbic acid, 7 mL 0.01 mol L -1 AgNO 3 , 30 mL 0.1 mol·L -1 NaOH aqueous solution, shaking for 1 min, and standing for...
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