Method for preparing egg white lysozyme and active protein by adopting coseparation
A technology of egg white lysozyme and active protein, which is applied in the fields of biology, medicine and food industry, can solve the problems that are not conducive to co-separation and preparation of egg white active protein, the degree of deep processing is backward, and the large-scale application is limited, so as to achieve easy large-scale industrialization Production, short production cycle, and the effect of protecting biological activity
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Embodiment 1
[0026] Add 100ml of fresh egg white, add 200ml of fresh deionized water, adjust the pH to about 5.5 with 1M hydrochloric acid, stir for 1 hour, and centrifuge at 8000rpm for 30min to remove the precipitate. The supernatant obtained by centrifugation was added with polyethylene glycol 200 until the mass concentration of polyethylene glycol was 10%, stirred evenly, left at room temperature for 1 hour, and centrifuged at 8000rpm for 30min to remove the precipitate. Add saturated ammonium sulfate solution to the supernatant obtained by centrifugation until the mass concentration of ammonium sulfate is 15%, stir evenly, place at room temperature for 1 hour, centrifuge at 6000rpm for 30min, and absorb the supernatant and the subnatant respectively. The supernatant was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 3000-5000Da at room temperature, and the ultrafiltrate was subjected to vacuum freeze-drying, and the obtained white powder was lysozyme ...
Embodiment 2
[0028] Add 100ml of fresh egg white, add 200ml of fresh deionized water, adjust the pH to about 5.5 with 1M hydrochloric acid, stir for 1 hour, and centrifuge at 8000rpm for 30min to remove the precipitate. The supernatant obtained by centrifugation was added with polyethylene glycol 200 until the mass concentration of polyethylene glycol was 30%, stirred evenly, left at room temperature for 1 hour, and centrifuged at 8000rpm for 30min to remove the precipitate. Add saturated ammonium sulfate solution to the supernatant obtained by centrifugation until the mass concentration of ammonium sulfate is 15%, stir evenly, place at room temperature for 1 hour, centrifuge at 6000rpm for 30min, and absorb the supernatant and the subnatant respectively. The supernatant was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 3000-5000Da at room temperature, and the ultrafiltrate was subjected to vacuum freeze-drying, and the obtained white powder was lysozyme ...
Embodiment 3
[0030] Add 100ml of fresh egg white, add 200ml of fresh deionized water, adjust the pH to about 5.5 with 1M hydrochloric acid, stir for 1 hour, and centrifuge at 8000rpm for 30min to remove the precipitate. The supernatant obtained by centrifugation was added with polyethylene glycol 20000 until the mass concentration of polyethylene glycol was 10%, stirred evenly, left at room temperature for 1 hour, and centrifuged at 8000rpm for 30min to remove the precipitate. Add saturated ammonium sulfate solution to the supernatant obtained by centrifugation until the mass concentration of ammonium sulfate is 15%, stir evenly, place at room temperature for 1 hour, centrifuge at 6000rpm for 30min, and absorb the supernatant and the subnatant respectively. The supernatant was ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 3000-5000Da at room temperature, and the ultrafiltrate was subjected to vacuum freeze-drying, and the obtained white powder was lysozym...
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