Method for enzymatically synthesizing lauroyl collagen amino acid triethanolamine

A technology of lauroyl collagen and triethanolamine, applied in the direction of fermentation, etc., can solve the problems of dark product color, single type of enzyme, and low purity, and achieve the effect of high product quality, mild nature, and high purity

Inactive Publication Date: 2015-07-01
广州市澳伦化妆品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, do not see the bibliographic disclosure of synthesizing this compound yet
[0003] Existing methods for synthesizing esters: such as the article "Synthesis and Performance Research of Triethanolamine Dilaurate" in the 7th issue of "Food and Oils" in 2008, the disclosed method is: using lauric acid and triethanolamine as raw materials, under the action of a catalyst Carry out esterification reaction, the product is separated and purified by silica gel column to obtain triethanolamine dilaurate, wherein the optimal conditions are: the catalyst is 2.5% toluenesulfonic acid, the lauric acid: triethanolamine molar ratio is 1.2:1, and the reaction temperature is 140°C , the reaction time is 14h, and the esterification rate is 86.95%. The main disadvantages of this method are: the chemical synthesis method is adopted, the reaction temperature is 140°C, and the toxic benzene series is used as the water-carrying agent, and the product has a darker color; For example, in the article "Kinetics and Thermodynamics of Non-aqueous Phase Enzymatic Synthesis of L-Profen Escorbate" in Volume 8, No. 6, "Bioprocessing Process" in 2010, the disclosed method is: using enzyme-catalyzed synthesis method to synthesize ibuprofen The optimal process of vitamin C ester is: rotating speed 200r / min, temperature 65°C, enzyme addition 5% (based on substrate mass fraction), substrate concentration 1mol / L, reaction 66h to reach equilibrium, product mass fraction 19.07 %
The main disadvantages of this method are: the type of enzyme used is single, the synthesis rate is low, and the purity is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for enzymatically synthesizing lauroyl collagen amino acid triethanolamine, the specific steps are as follows.

[0023] (1) Enzymatic hydrolysis and filtration

[0024] Using commercially available collagen protein powder as raw material, pump a certain amount of pure water into the reaction kettle, heat to the temperature of the pure water in the reaction kettle is 55 ° C, according to alkaline protease (kg): flavor protease (kg): collagen protein powder ( The ratio of kg): pure water volume (L) is 1: 2.5: 110: 550. First add collagen powder to the reaction kettle, stir for 25 minutes at a stirring speed of 100 r / min, then add alkaline protease, and stir to react After 120 minutes, flavor protease was finally added, stirred and hydrolyzed for 2.5 hours, and the hydrolyzed collagen amino acid solution was prepared. The hydrolyzed collagen amino acid solution is pumped into a microporous filter with a pore size of 1.4 μm for filtration, and the filter residue a...

Embodiment 2

[0032] A method for enzymatically synthesizing lauroyl collagen amino acid triethanolamine, same as Example 1, wherein:

[0033] In the (1) step, the temperature of the pure water in the reactor is heated to 50°C, and the ratio of alkaline protease (kg): flavor protease (kg): collagen powder (kg): pure water volume (L) is 1:1 : 100: 500, the stirring speed is 60r / min, after stirring for 20min, add alkaline protease, after stirring for 50min, add flavor protease, stir for 2h for hydrolysis. The microfiltration aperture is 1.0 μm, the ultrafiltration molecular weight cut-off is 800Da, the ultrafiltration pressure is 0.05Mpa, and the retentate:filtrate volume ratio is 1:7.

[0034] In step (2), the reverse osmosis pressure is 0.2Mpa, and it is concentrated until the amino acid concentration of the hydrolyzed collagen reaches 15%. The air inlet temperature of the spray dryer is 135°C, the outlet air temperature is 85°C, and the rotation speed is 12000r / min.

[0035] In step (3),...

Embodiment 3

[0038] A method for enzymatically synthesizing lauroyl collagen amino acid triethanolamine, same as Example 1, wherein:

[0039] In step (1), the temperature of the purified water in the reactor is heated to 60°C, and the ratio of alkaline protease (kg): flavor protease (kg): collagen powder (kg): pure water volume (L) is 1:3 : 120: 650, the stirring speed is 120r / min, after stirring for 30min, add alkaline protease, after stirring for 150min, add flavor protease, stir for 3h for hydrolysis. The pore size of the microfiltration is 1.5 μm, the ultrafiltration molecular weight cut-off is 2000 Da, the ultrafiltration pressure is 0.2 Mpa, and the retentate: filtrate volume ratio is 1:9.

[0040] In step (2), the reverse osmosis pressure is 0.6Mpa, and it is concentrated until the amino acid concentration of the hydrolyzed collagen reaches 20%. The air inlet temperature of the spray dryer is 150°C, the outlet air temperature is 95°C, and the rotation speed is 18000r / min.

[0041]...

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PUM

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Abstract

The invention discloses a method for enzymatically synthesizing lauroyl collagen amino acid triethanolamine, and relates to a preparation method of lauroyl collagen amino acid triethanolamine. The method comprises steps of: carrying out enzyme hydrolysis on collagen powder sold in market and serving as a raw material, filtering, concentrating, drying, esterifying, filtering and diluting so as to obtain a finished product. The method has the characteristics of moderate synthesizing conditions, high synthesizing rate, simplicity in operation, low cost, safety in cleaning and the like. The lauroyl collagen amino acid triethanolamine prepared by adopting the method has high purity, moderate properties and biological degradability and can be widely applied to the fields of cosmetic, medicines, health-care products, textile and the like.

Description

1. Technical field [0001] The invention belongs to the technical field of preparing surfactant lauroyl collagen amino acid triethanolamine, in particular to a preparation method of lauroyl collagen amino acid triethanolamine. 2. Background technology [0002] Lauroyl collagen amino acid triethanolamine is a mild surfactant synthesized from hydrolyzed collagen amino acid, lauric acid and triethanolamine as the main raw materials. Because the foam of this product is fine and rich, the collagen amino acid in it is highly compatible with the skin mainly composed of collagen. And, good biocompatibility, low irritation, and the product is biodegradable and environmentally friendly, so it is widely used in high-end shampoo, bath, cleansing and other products, as the main foaming agent and foaming aid, the demand gradually increase. However, there has been no literature disclosure on the synthesis of this compound. [0003] Existing methods for synthesizing esters: such as the art...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00
Inventor 彭元鸿周小华冯琳万玉萍
Owner 广州市澳伦化妆品有限公司
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