Method for producing xylosic acid through xylose whole-cell catalysis by direct oxygen introduction and pressurization
A xylonic acid, whole cell technology, applied in the fields of bioengineering and chemical engineering, can solve problems such as overflow, production operation and product quality impact, foam, etc., achieve simple production operation and control, eliminate foam phenomenon, and improve reaction rate. and the effect of reaction concentration
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[0023] Example 1
[0024] In a 3.0L mechanically ventilated reaction tank, add 1.0L xylose liquid to the initial monosaccharide concentration of 150g / L, add 40g / L calcium carbonate to adjust the pH of the reaction system from 5.0 to 7.5, start the stirring speed at 500r / min, then Enter the activated Gluconobacter oxydans to the cell concentration OD 600nm =1.0. Open the exhaust gas discharge valve and directly introduce oxygen (volume concentration not less than 90%) for 10 minutes to replace the air in the reaction system, and then seal the reaction system for catalytic reaction. Adjust the oxygen supply pressure of the oxygen source to maintain the internal pressure of the reaction system at 0.01~0.10 MPa.
[0025] Real-time monitoring of reaction system CO 2 Concentration to CO 2 When the concentration content in the exhaust gas of the reaction system reaches 5% (volume ratio), reduce the stirring speed to 80r / min, open the exhaust valve of the reaction tank, and pass oxygen fo...
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[0027] Example 2
[0028] In a 100 L mechanically ventilated reactor, add 60 L of xylose liquid to the initial monosaccharide concentration of 100g / L, add 35g / L of calcium carbonate to adjust the pH of the reaction system to 5.0~7.5, start the stirring speed at 500r / min, and connect The activated Gluconobacter oxydans to the cell concentration of OD 600nm = 1.0. Open the exhaust gas discharge valve and directly introduce oxygen (volume concentration not less than 90%) for 15 minutes to replace the air in the reaction system, and then seal the reaction system for catalytic reaction. Adjust the oxygen supply pressure of the oxygen source to maintain the internal pressure of the reaction system at 0.01~0.03MPa.
[0029] Real-time monitoring of reaction system CO 2 Concentration to CO 2 When the concentration content in the exhaust gas of the reaction system reaches 5% (volume ratio), reduce the stirring speed to 50r / min, open the exhaust valve of the reaction tank, and discharge the...
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