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Method for producing xylosic acid through xylose whole-cell catalysis by direct oxygen introduction and pressurization

A xylonic acid, whole cell technology, applied in the fields of bioengineering and chemical engineering, can solve problems such as overflow, production operation and product quality impact, foam, etc., achieve simple production operation and control, eliminate foam phenomenon, and improve reaction rate. and the effect of reaction concentration

Active Publication Date: 2013-07-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the concentration of xylose or xylonic acid in the reaction system is as high as 30%, the viscosity of cells, sugar and sugar acid and the influence of large ventilation will cause serious foam problems, even if a large amount of disinfectant is used. The foaming agent also has the risk of overflowing the tank
This phenomenon will be further aggravated in the xylose catalysis process of lignocellulose hydrolyzate containing more complex components, such as pectin, lignin, and lipid extracts, which will have a serious impact on production operations and product quality

Method used

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  • Method for producing xylosic acid through xylose whole-cell catalysis by direct oxygen introduction and pressurization
  • Method for producing xylosic acid through xylose whole-cell catalysis by direct oxygen introduction and pressurization

Examples

Experimental program
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Effect test

Embodiment 1

[0024] In a 3.0L mechanically ventilated reaction tank, add 1.0L xylose solution to an initial monosaccharide concentration of 150g / L, add 40g / L calcium carbonate to adjust the pH of the reaction system to 5.0~7.5, start the stirring speed at 500r / min, then Inject activated Gluconobacter oxydans to a cell concentration of OD 600nm =1.0. Open the exhaust gas discharge valve, directly feed oxygen (volume concentration not less than 90%) for 10 minutes to replace the air in the reaction system, and then seal the reaction system for catalytic reaction. Adjust the oxygen supply pressure of the oxygen source to maintain the internal pressure of the reaction system at 0.01~0.10MPa.

[0025] Real-time monitoring of reaction system CO 2 concentration, to CO 2 When the concentration in the exhaust gas of the reaction system reaches 5% (volume ratio), reduce the stirring speed to 80r / min, open the exhaust valve of the reaction tank, and pass oxygen for 5~15min to replace the CO in the...

Embodiment 2

[0028] In a 100 L mechanically ventilated reaction tank, add 60 L of xylose solution to an initial monosaccharide concentration of 100 g / L, add 35 g / L of calcium carbonate to adjust the pH of the reaction system to 5.0~7.5, start the stirring speed at 500 r / min, and insert Activated Gluconobacter oxydans to a cell concentration of OD 600nm = 1.0. Open the exhaust gas discharge valve, directly introduce oxygen (volume concentration not less than 90%) for 15 minutes to replace the air in the reaction system, and then seal the reaction system for catalytic reaction. Adjust the oxygen supply pressure of the oxygen source to maintain the internal pressure of the reaction system at 0.01~0.03MPa.

[0029] Real-time monitoring of reaction system CO 2 concentration, to CO 2 When the concentration in the exhaust gas of the reaction system reaches 5% (volume ratio), reduce the stirring speed to 50r / min, open the exhaust valve of the reaction tank, and discharge the CO in the reaction...

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Abstract

The invention discloses a method for producing xylosic acid through xylose whole-cell catalysis by direct oxygen introduction and pressurization. The method comprises the following steps that: oxygen is directly introduced into a xylose liquid deep reaction system for air replacement in the mixing condition, the gluconobacter oxydans is then inoculated into the system, the sealed reaction system is pressurized to 0.01-0.10MPa with oxygen to carry out xylose whole-cell catalysis for the production of the xylosic acid; the carbon dioxide content generated by the neutralization reaction of the reaction system is monitored, when the carbon dioxide concentration in the waste gas exceeds 5%, mixing intensity is reduced, the reaction system is started, the waste gas in the reaction system is replaced through the oxygen, then the reaction system is again sealed, and the stirring strength is recovered to continue the reaction. By adopting the method disclosed by the invention, the severe foam problem caused by direct air introduction in the production process can be completely solved, no defoamer needs to be added, product refining procedure is greatly simplified, product quality is improved, the product concentration of the xylosic acid and the yield of the unit system are respectively improved by 50% and 66% due to the high concentration oxygen introduction, the xylosic acid product concentration reaches 600g / L, and xylose utilization rate is higher than 98%.

Description

technical field [0001] The invention relates to the fields of bioengineering and chemical engineering, in particular to a method for directly producing xylonic acid by catalyzing xylose whole cells through oxygen and pressurization. Background technique [0002] As an emerging bio-based platform chemical, xylonic acid can be widely used in the production of cement superplasticizers, dispersants and slow-release agents, concrete adhesives, plasticizers, glass cleaners, metallurgical rust removers, Metal ion chelating agents, insecticides, textile bleaching aids, icy viscose fiber blends, etc. are a new way out for efficient biotransformation and utilization of xylose. [0003] At present, the mass concentration of xylose produced from xylose produced by whole-cell catalytic conversion of xylose by microorganisms such as Gluconobacter oxidans can reach 30%, the sugar utilization rate and conversion rate are both over 98%, and the unit volume productivity reaches 3.6 g / (L.h ),...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12R1/01
Inventor 徐勇周鑫李振荣麻艺聪勇强余世袁陈牧朱均均李鑫蒋伶俐莫逸仙
Owner NANJING FORESTRY UNIV
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