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Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof

A fiber composite and chitosan technology, applied in medical science, absorbent pads, bandages, etc., can solve the problems of insufficient mechanical properties of collagen-based hemostatic agents, failure to meet market requirements, insufficient purity of type I collagen, etc., and achieve good biological Effects of degradability, promotion of wound healing, good biocompatibility

Active Publication Date: 2015-02-04
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The existing collagen-based hemostatic agent has insufficient mechanical properties and poor adhesion, which causes unnecessary troubles to the operation;
[0007] 2. The existing collagen-based hemostatic agents have general hemostatic performance and single function, which can no longer meet the requirements of the market;
[0008] 3. The type I collagen in the existing collagen-based hemostatic agent monomer material is not pure enough, and there is still a certain degree of immunogenicity, which may cause immeasurable harm to patients when used in clinical practice

Method used

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  • Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof
  • Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof
  • Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029](1) Type I collagen extraction: Take 10 parts by weight of medical biological skin, cut it into small pieces of 0.5cm×0.5cm, wash it with ultrapure water for 5 times, and then soak it in 0.05mol / L Tris, 1mol / L L NaCl, in a Tris-NaCl buffer solution with a pH of 7.5, place it in an ultrasonic cleaner with a frequency of 20kHz, and act for 2 hours; pour off the buffer solution, rinse the skin twice with distilled water; add 30 times of 0.5M acetic acid solution, soaked for 2 hours, crushed and homogenized with a homogenizer at a constant temperature of 4°C, and then transferred to a reaction kettle, adding 0.2 parts by weight of pepsin; under ultrasonic conditions with a frequency of 30kHz, slowly stirred at 4°C Enzyme hydrolysis for 36 hours, and act for 1 hour every 3 hours; after the reaction is completed, stop stirring, filter the reaction solution, adjust the pH of the filtrate to 7.0, add ammonium sulfate powder with a final concentration of 1.5mol / L, and let it stand...

Embodiment 2

[0036] (1) Type I collagen extraction: Take 10 parts by weight of medical biological skin, cut it into small pieces of 0.5cm×0.5cm, wash it with ultrapure water for 5 times, and then soak it in 0.05mol / L Tris, 1mol / L L NaCl, in a Tris-NaCl buffer solution with a pH of 7.5, place it in an ultrasonic cleaner with a frequency of 30 kHz, and act for 2 hours; pour off the buffer solution, rinse the skin twice with distilled water; add 40 times of 0.5M acetic acid Solution, soaked for 2 hours, crushed and homogenized with a homogenizer at a constant temperature of 4°C, then transferred to a reaction kettle, added 0.2 parts by weight of pepsin, and slowly stirred the enzyme at 4°C under ultrasonic conditions with a frequency of 30kHz Solution for 24 hours, every 3 hours for 1 hour. After the reaction is completed, stop stirring, filter the reaction solution with suction, adjust the pH of the filtrate to 7.5, add ammonium sulfate powder with a final concentration of 1.5mol / L, and let ...

Embodiment 3

[0043] (1) Type I collagen extraction: Take 10 parts by weight of medical biological skin, cut it into small pieces of 0.5cm×0.5cm, wash it with ultrapure water for 5 times, and then soak it in 0.05mol / L Tris, 1mol / L L NaCl, in a Tris-NaCl buffer solution with a pH of 7.5, place it in an ultrasonic cleaner with a frequency of 30 kHz, and act for 2 hours; pour off the buffer solution, rinse the skin twice with distilled water; add 50 times of 0.5M acetic acid solution, soaked for 2 hours, crushed and homogenized with a homogenizer at a constant temperature of 4°C, and then transferred to a reaction kettle, adding 0.2 parts by weight of pepsin; under the condition of ultrasonic waves with a frequency of 20kHz, the enzyme was slowly stirred at 4°C Decompose for 36 hours, and act for 1 hour every 3 hours; after the reaction is completed, stop stirring, filter the reaction solution with suction, adjust the pH of the filtrate to 7.3, add ammonium sulfate powder with a final concentra...

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Abstract

The invention discloses a collagen / chitosan micro-nano fiber composite hemostatic membrane material and a preparation method thereof. The preparation method is characterized by comprising the following steps of: extracting a medical biological skin sheet serving as a raw material by an ultrasonic technology to prepare I type collagen; mixing hexafluoroisopropanol and acetic acid according to different volume ratios to prepare a spinning solvent; mixing the prepared I type collagen and chitosan in a certain mass ratio and adding the mixture into the spinning solvent; stirring in an ultrasonic cleaner at room temperature until the material is transparent to prepare electrostatic spinning mother liquid at the concentration of 4 to 10 percent; injecting the electrostatic spinning mother liquid into an electrostatic spinning machine and performing electrostatic spinning to obtain a collagen / chitosan micro-nano fiber composite membrane; soaking the collagen / chitosan micro-nano fiber composite membrane into natural biomacromolecules and Chinese herbal medicines sequentially; and freeze-drying to obtain the collagen / chitosan micro-nano fiber composite hemostatic membrane material. The collagen / chitosan micro-nano fiber composite hemostatic membrane material has excellent biocompatibility, biodegradability, adhesion property and the like, can quickly stop bleeding, resist inflammation, ease pain and promote wound healing, and can be applied to trauma hemostasis and repair and general civil hemostasis emergency treatment.

Description

technical field [0001] The invention relates to a hemostatic agent which has excellent properties such as good biocompatibility, biodegradability and adhesion, and can quickly stop bleeding, anti-inflammatory and analgesic, and promote wound compounding. Background technique [0002] Bleeding poses a great danger to human life, and the search for fast and effective hemostatic agents has always been a research hotspot at home and abroad. In various surgical operations or trauma treatments, wound bleeding and isolation from the outside world to avoid infection have a great impact on the wound healing of patients. In war, according to statistics, bleeding is the main cause of death within 48 hours after injury, accounting for 80% of all traumatic accidents. Excessive traumatic blood loss will cause pain, shock, coma and even death of the wounded. Therefore, hemostatic materials need to stop bleeding quickly and effectively, and must also have anti-inflammatory and analgesic ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/44
Inventor 但年华刘新华但卫华胡杨刘婷
Owner SICHUAN UNIV
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