High-strength high-tenacity protein molecule imprinting hybrid gel film and preparation method thereof

Abstract

The invention relates to a high-strength high-tenacity protein molecule imprinting hybrid gel film and a preparation method thereof. The preparation method comprises the following steps of: with sodium alginate as a molecular frame, a water-soluble compound as a pore-forming agent as well as acrylamide and derivants thereof as functional monomers, dissolving the functional monomers and a protein template to form a complex, adding a chemical crosslinking agent and an ionic crosslinking agent, and initiating the polymerization to form high-strength and high-tenacity double-crosslinked network hydrogel containing protein; and eluting the pore-forming agent and the protein template to obtain a porous hybrid gel film, wherein the porous hybrid gel film has larger adsorbing capacity and better adsorptive selectivity on the protein template. The performances of the gel can be changed by using various environment stimulating factors, such as temperature, pH, solvent property, ion strength and special chemical simulation, and therefore, the interaction between the molecule imprinting hybrid film and protein can be controlled. The preparation method is simple in process and low in cost; and the obtained porous hybrid gel film has a favorable application prospect in the fields including protein separation and analysis, protein control and release, tissue engineering and the like.

Description

technical field [0001] The invention relates to a high-strength and high-toughness protein molecularly imprinted hybrid gel film and a preparation method thereof, belonging to the fields of biological materials, composite materials and polymer materials. Background technique [0002] Molecular imprinting is a technique for preparing polymers (molecularly imprinted polymers (MIPs)) with specific selectivity for specific target molecules (template molecules). MIPs have the characteristics of predictability, identification and practicability, and have broad application prospects in solid phase extraction, immunoassay, biomaterial simulation and sensors. In recent years, the research on small molecule imprinting technology has achieved great success, but the imprinting of large molecules such as proteins has encountered unprecedented challenges [nature materials.2010, 9(8): 612-614; Biomaterials. ): 3008-3020]. The difficulty of protein molecular imprinting is related to the p...

AI Technical Summary

Problems solved by technology

[0009] Aiming at the deficiencies of the prior art, the technical problems to be solved by the present invention are problems such as difficult diffusion of protein during western blotting, low strength of western blotting hydrogel, difficulty in maintaining imprinted holes in the hydrogel, etc.

[0010] The technical solution of the present invention to solve the problems of difficult diffusion of protein, low strength of protein imprinted hydrogel, and difficult maintenance of imprinted holes in hydrogel in the process of protein imprinting is to design a high-strength and high-toughness protein molecularly imprinted hybrid gel film