High-strength high-tenacity protein molecule imprinting hybrid gel film and preparation method thereof

A hybrid gel and molecular imprinting technology, applied in the fields of composite materials, polymer materials, and biological materials, can solve the problems of difficult diffusion of proteins, low strength of protein imprinted hydrogels, and difficulty in maintaining imprinted holes, achieving low cost, Good application prospects, the effect of improving strength and toughness

Active Publication Date: 2013-08-21
广州市赛凌医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the deficiencies of the prior art, the technical problems to be solved by the present invention are problems such as difficult diffusion of protein during western blotting, low strength of western blotting hydrogel, difficulty in maintaining imprinted holes in the hydrogel, etc.
[0010] The technical so

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. A bovine hemoglobin molecularly imprinted polyisopropylacrylamide / calcium alginate hybrid gel membrane and its preparation method

[0023] a) Weigh 0.005g bovine hemoglobin, 5g isopropylacrylamide, 0.5g sodium alginate, ethylene glycol dimethacrylate with a mass percentage of isopropylacrylamide of 0.15%, and isopropylacrylamide with a mass percentage of 1%. Dissolve polyvinylpyrrolidone in 50ml of deionized water together, stir and dissolve evenly, then add nano-silica with isopropylacrylamide mass percentage of 100%, stir and ultrasonically disperse evenly to obtain a casting solution, and place it at 20°C Reserved in a sealed sterile container;

[0024] b) preparing a calcium nitrate aqueous solution with a mass percentage of calcium ions of 0.01%, as an ion cross-linking agent;

[0025] c) Add 5% ammonium persulfate by mass percentage of isopropylacrylamide to the casting liquid prepared in step a), sodium bisulfite and 2% by mass percentage of isopropyl...

Embodiment 2

[0027] Example 2. A bovine serum albumin molecularly imprinted polyisopropylacrylamide / calcium alginate hybrid gel membrane and its preparation method

[0028] a) Weigh 5g of bovine serum albumin, 15g of acrylamide, 2g of sodium alginate, divinylbenzene of 0.03%-0.15% by mass of acrylamide, polyethylene glycol of 100% by mass of acrylamide, and dissolve them together in 100ml Stir and dissolve in deionized water evenly, then add nano-hydroxyapatite with 10% mass percentage of acrylamide, stir and ultrasonically disperse evenly to obtain a casting solution, and place it in a closed sterile container at 20°C for later use;

[0029] b) preparing an aqueous solution of calcium chloride with a mass percentage of calcium ions of 10% as an ion cross-linking agent;

[0030] c) adding 0.1% ammonium persulfate of acrylamide mass percentage, 0.1% sodium bisulfite of acrylamide mass percentage and 0.01% tetramethylethylenediamine of acrylamide mass percentage to the casting solution prepa...

Embodiment 3

[0032] Example 3. A lysozyme molecularly imprinted polydiacetone acrylamide / barium alginate hybrid gel film and its preparation method

[0033] a) Weigh 0.5g of lysozyme, 10g of diacetone acrylamide, 1g of sodium alginate, 0.05% by mass of diacetone acrylamide, N, N'-methylene bisacrylamide, 100% by mass of diacetone acrylamide Dissolve polyvinyl alcohol in 80ml deionized water together, stir to dissolve evenly, then add nanoclay with 5% mass percentage of diacetone acrylamide, stir and ultrasonically disperse evenly to obtain a casting solution, and place it in a closed sterile container at 2°C in standby;

[0034] b) preparing an aqueous barium chloride solution with a mass percentage of barium ions of 2%, as an ion crosslinking agent;

[0035] c) add diacetone acrylamide mass percentage 0.5% ammonium persulfate to the casting solution prepared in step a), diacetone acrylamide mass percentage 0.5% sodium bisulfite and diacetone acrylamide mass percentage 0.05% diacetone per...

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Abstract

The invention relates to a high-strength high-tenacity protein molecule imprinting hybrid gel film and a preparation method thereof. The preparation method comprises the following steps of: with sodium alginate as a molecular frame, a water-soluble compound as a pore-forming agent as well as acrylamide and derivants thereof as functional monomers, dissolving the functional monomers and a protein template to form a complex, adding a chemical crosslinking agent and an ionic crosslinking agent, and initiating the polymerization to form high-strength and high-tenacity double-crosslinked network hydrogel containing protein; and eluting the pore-forming agent and the protein template to obtain a porous hybrid gel film, wherein the porous hybrid gel film has larger adsorbing capacity and better adsorptive selectivity on the protein template. The performances of the gel can be changed by using various environment stimulating factors, such as temperature, pH, solvent property, ion strength and special chemical simulation, and therefore, the interaction between the molecule imprinting hybrid film and protein can be controlled. The preparation method is simple in process and low in cost; and the obtained porous hybrid gel film has a favorable application prospect in the fields including protein separation and analysis, protein control and release, tissue engineering and the like.

Description

technical field [0001] The invention relates to a high-strength and high-toughness protein molecularly imprinted hybrid gel film and a preparation method thereof, belonging to the fields of biological materials, composite materials and polymer materials. Background technique [0002] Molecular imprinting is a technique for preparing polymers (molecularly imprinted polymers (MIPs)) with specific selectivity for specific target molecules (template molecules). MIPs have the characteristics of predictability, identification and practicability, and have broad application prospects in solid phase extraction, immunoassay, biomaterial simulation and sensors. In recent years, the research on small molecule imprinting technology has achieved great success, but the imprinting of large molecules such as proteins has encountered unprecedented challenges [nature materials.2010, 9(8): 612-614; Biomaterials. ): 3008-3020]. The difficulty of protein molecular imprinting is related to the p...

Claims

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Application Information

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IPC IPC(8): C08L33/24C08L33/26C08L5/04C08F220/58C08F220/54C08F220/56C08J9/26C08J3/24C08J5/18
Inventor 赵孔银崔文葵魏俊富季舒骏张方彦
Owner 广州市赛凌医药科技有限公司
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