Processing method of compound hypha powder
A technology of composite bacteria and silk powder, applied in botany equipment and methods, fertilizer mixture, gardening, etc., can solve the problems of easy loss of nutrients, achieve good effect, high promotion value, and high survival rate of bacteria
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Embodiment 1
[0039] 1. Preparation of culture medium
[0040] (1) Preparation of grain medium
[0041] Squeeze, crush and mix 100kg of wheat, corn, millet, and soybeans at a mass ratio of 1:1.5:0.6:1, add water to a water content of 37%, and pack them into fermentation bottles, then perform high-temperature sterilization and sterilization Conditions: 121°C, 40min, when the temperature of the medium drops to 27°C after sterilization, put it into the sterile inoculation room for inoculation;
[0042] (2) Preparation of slant medium
[0043] Preparation of sweet potato extract: Wash and peel the sweet potatoes, add them to the cooking pot according to the ratio of sweet potatoes: water = 1:0.8, boil and keep boiling for 15 minutes on low heat, cook the sweet potatoes until they are rotten, filter through 4 layers of gauze , take its filtrate, which is the sweet potato extract. The same below.
[0044] According to the mass percentage of glucose 15%, soybean flour 15%, corn flour 10%, whea...
Embodiment 2
[0064] 1. Preparation of culture medium
[0065] (1) Preparation of grain medium
[0066] Squeeze, crush and mix 100kg of wheat, corn, millet, and soybeans at a mass ratio of 1:1.8:0.8:1.1, add water to a water content of 38%, and pack them into fermenting bottles, then sterilize at high temperature, and sterilize Conditions: 121°C, 40min, when the temperature of the medium drops to 30°C after sterilization, put it into the sterile inoculation room for inoculation;
[0067] (2) Preparation of slant medium
[0068] 20% by mass percentage of glucose, 40% of soybean flour, 11% of corn flour, 12% of wheat flour, 0.3% of potassium dihydrogen phosphate, 0.2% of magnesium sulfate, 3% of agar, 6.5% of peptone, and the rest is sweet potato extract. pH 7.0, dispense into test tubes, mix well and then sterilize at high temperature. Sterilization conditions: 122°C, 50 minutes; take out the test tubes 13 minutes after the end of sterilization, place them on an inclined plane while hot, a...
Embodiment 3
[0088] 1. Preparation of culture medium
[0089] (1) Preparation of grain medium
[0090] Squeeze, crush and mix 100kg of wheat, corn, millet, and soybeans according to the mass ratio of 1:2:1:1.2, add water to 40% water content, pack them into fermentation bottles, and then perform high-temperature sterilization to sterilize Conditions: 123°C, 60min, when the temperature of the culture medium drops to 35°C after sterilization, put it into the sterile inoculation room for inoculation;
[0091] (2) Preparation of slant medium
[0092] According to the mass percentage of glucose 18%, corn flour 12%, wheat flour 13%, soybean flour 38%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.2%, agar 4%, peptone 7%, and the rest is sweet potato extract. pH 7.0, dispense into test tubes, mix well and then sterilize at high temperature. Sterilization conditions: 123°C, 60 minutes; take out the test tubes 15 minutes after the end of sterilization, place them on a slope while they ...
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